Capybara microvirus Cap1_SP_168
Average proteome isoelectric point is 5.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W821|A0A4P8W821_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_168 OX=2585403 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.24 FSSRR6 pKa = 11.84 YY7 pKa = 5.65 EE8 pKa = 4.07 HH9 pKa = 7.18 PVTPITNPGTEE20 pKa = 3.79 FRR22 pKa = 11.84 NDD24 pKa = 3.28 YY25 pKa = 8.8 QAHH28 pKa = 5.7 YY29 pKa = 10.6 DD30 pKa = 3.67 EE31 pKa = 6.54 DD32 pKa = 4.43 GVLVTVKK39 pKa = 10.62 VGEE42 pKa = 4.19 TNIYY46 pKa = 11.04 QMIQSYY52 pKa = 10.87 ADD54 pKa = 3.32 SVDD57 pKa = 2.93 IHH59 pKa = 7.54 ILLEE63 pKa = 4.2 QFGSGYY69 pKa = 11.14 AEE71 pKa = 3.71 ALNRR75 pKa = 11.84 FEE77 pKa = 5.95 GFYY80 pKa = 11.08 ADD82 pKa = 4.79 ISEE85 pKa = 5.3 LPDD88 pKa = 3.46 NYY90 pKa = 10.74 VGMLNTIEE98 pKa = 4.25 TTRR101 pKa = 11.84 SYY103 pKa = 11.21 FDD105 pKa = 4.57 KK106 pKa = 10.94 LPADD110 pKa = 3.38 IRR112 pKa = 11.84 SKK114 pKa = 10.95 FNNDD118 pKa = 2.46 FSQFLVASQQPDD130 pKa = 3.62 FLSNFAPAVPISNMYY145 pKa = 9.98 VPGEE149 pKa = 4.05 NVPGEE154 pKa = 4.21 NVPGEE159 pKa = 4.16 NVPGEE164 pKa = 4.42 KK165 pKa = 10.18 EE166 pKa = 4.0 NQQ168 pKa = 3.16
Molecular weight: 18.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.121
IPC2_protein 4.291
IPC_protein 4.215
Toseland 4.037
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.05
Rodwell 4.05
Grimsley 3.948
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.431
Thurlkill 4.062
EMBOSS 4.062
Sillero 4.317
Patrickios 2.816
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.238
Protein with the highest isoelectric point:
>tr|A0A4P8W5K6|A0A4P8W5K6_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_168 OX=2585403 PE=4 SV=1
MM1 pKa = 7.18 TCYY4 pKa = 10.43 HH5 pKa = 6.5 PLKK8 pKa = 10.64 RR9 pKa = 11.84 FTTGFNPEE17 pKa = 3.91 TGKK20 pKa = 11.07 SIGKK24 pKa = 8.06 VVPYY28 pKa = 10.39 RR29 pKa = 11.84 VDD31 pKa = 3.24 HH32 pKa = 6.14 LEE34 pKa = 3.64 KK35 pKa = 10.43 RR36 pKa = 11.84 YY37 pKa = 10.7 GDD39 pKa = 3.37 DD40 pKa = 4.05 RR41 pKa = 11.84 FYY43 pKa = 11.3 QVYY46 pKa = 10.76 DD47 pKa = 3.44 PVDD50 pKa = 3.46 YY51 pKa = 7.82 TTYY54 pKa = 10.17 TINGTKK60 pKa = 9.97 FEE62 pKa = 4.31 NMPLKK67 pKa = 10.61 PSDD70 pKa = 3.2 VRR72 pKa = 11.84 IYY74 pKa = 10.96 SDD76 pKa = 4.3 YY77 pKa = 11.44 EE78 pKa = 4.05 VIPCGKK84 pKa = 10.37 CIGCRR89 pKa = 11.84 LDD91 pKa = 3.48 YY92 pKa = 11.29 SRR94 pKa = 11.84 MWSSRR99 pKa = 11.84 LMAEE103 pKa = 4.63 LPYY106 pKa = 10.6 HH107 pKa = 6.1 HH108 pKa = 6.73 EE109 pKa = 3.91 AWFLTLTYY117 pKa = 10.95 NDD119 pKa = 4.11 LCLPSHH125 pKa = 5.86 IVEE128 pKa = 4.24 RR129 pKa = 11.84 DD130 pKa = 3.21 GKK132 pKa = 10.67 LVEE135 pKa = 4.14 THH137 pKa = 6.52 SLVKK141 pKa = 10.55 KK142 pKa = 10.05 HH143 pKa = 6.02 FQDD146 pKa = 3.16 FMKK149 pKa = 10.42 RR150 pKa = 11.84 LRR152 pKa = 11.84 RR153 pKa = 11.84 RR154 pKa = 11.84 FPDD157 pKa = 3.66 GSIKK161 pKa = 10.52 YY162 pKa = 7.41 YY163 pKa = 10.8 CCGEE167 pKa = 4.16 YY168 pKa = 10.47 GSKK171 pKa = 10.46 SMRR174 pKa = 11.84 PHH176 pKa = 4.77 YY177 pKa = 10.13 HH178 pKa = 6.99 AILFFDD184 pKa = 5.04 SSLQDD189 pKa = 3.29 YY190 pKa = 8.01 VFPNGVINTNKK201 pKa = 9.44 LHH203 pKa = 6.41 KK204 pKa = 10.38 VQDD207 pKa = 3.81 GYY209 pKa = 11.32 RR210 pKa = 11.84 YY211 pKa = 9.64 YY212 pKa = 11.36 VNRR215 pKa = 11.84 LIDD218 pKa = 3.71 QLWYY222 pKa = 11.07 VSDD225 pKa = 4.71 CPDD228 pKa = 2.94 VSAGFHH234 pKa = 6.45 YY235 pKa = 9.28 LTQVTYY241 pKa = 8.59 EE242 pKa = 4.16 TICYY246 pKa = 6.19 VTQYY250 pKa = 8.28 VTKK253 pKa = 10.41 KK254 pKa = 10.55 LNGDD258 pKa = 3.47 AASIYY263 pKa = 9.84 QEE265 pKa = 3.94 YY266 pKa = 10.72 DD267 pKa = 3.25 LEE269 pKa = 4.8 PEE271 pKa = 4.26 FSLISTKK278 pKa = 10.19 PALGRR283 pKa = 11.84 QFYY286 pKa = 9.58 EE287 pKa = 3.68 EE288 pKa = 4.44 HH289 pKa = 6.54 KK290 pKa = 11.16 AEE292 pKa = 4.66 IYY294 pKa = 11.04 ADD296 pKa = 3.47 DD297 pKa = 4.18 KK298 pKa = 11.52 FVFEE302 pKa = 5.11 SNGKK306 pKa = 8.5 PVIFRR311 pKa = 11.84 PPAYY315 pKa = 9.65 YY316 pKa = 10.63 DD317 pKa = 3.21 HH318 pKa = 7.17 LFDD321 pKa = 5.32 IEE323 pKa = 4.3 APEE326 pKa = 4.38 IMSQKK331 pKa = 9.57 KK332 pKa = 10.06 DD333 pKa = 3.3 KK334 pKa = 10.89 RR335 pKa = 11.84 KK336 pKa = 9.6 EE337 pKa = 3.49 LAINHH342 pKa = 5.44 VDD344 pKa = 3.16 ALLRR348 pKa = 11.84 QTDD351 pKa = 3.28 KK352 pKa = 11.23 DD353 pKa = 4.06 YY354 pKa = 10.93 PAILADD360 pKa = 3.5 QEE362 pKa = 4.77 SAFKK366 pKa = 10.78 SRR368 pKa = 11.84 ASSRR372 pKa = 11.84 MKK374 pKa = 10.68 AKK376 pKa = 10.32 YY377 pKa = 9.92 NKK379 pKa = 10.08 AKK381 pKa = 9.9 GRR383 pKa = 11.84 RR384 pKa = 11.84 VV385 pKa = 3.1
Molecular weight: 45.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.375
IPC2_protein 7.658
IPC_protein 7.512
Toseland 7.249
ProMoST 8.039
Dawson 8.2
Bjellqvist 8.492
Wikipedia 8.156
Rodwell 8.229
Grimsley 7.322
Solomon 8.273
Lehninger 8.273
Nozaki 8.565
DTASelect 8.273
Thurlkill 8.317
EMBOSS 8.404
Sillero 8.609
Patrickios 3.783
IPC_peptide 8.273
IPC2_peptide 7.468
IPC2.peptide.svr19 7.659
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1479
107
598
295.8
33.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.384 ± 2.346
1.014 ± 0.42
6.829 ± 0.624
5.477 ± 0.694
5.341 ± 0.369
6.558 ± 0.775
2.366 ± 0.392
5.341 ± 0.477
5.139 ± 0.907
6.694 ± 0.471
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.164 ± 0.105
5.274 ± 1.038
5.003 ± 0.732
3.854 ± 0.38
4.124 ± 0.736
7.911 ± 0.99
5.274 ± 0.198
5.68 ± 0.77
0.947 ± 0.184
6.626 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here