Tenacibaculum phage PTm5
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 306 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S9HXX8|A0A5S9HXX8_9CAUD Uncharacterized protein OS=Tenacibaculum phage PTm5 OX=2547426 PE=4 SV=1
MM1 pKa = 8.46 DD2 pKa = 6.63 DD3 pKa = 5.05 DD4 pKa = 4.3 LVNNIFIACEE14 pKa = 3.54 QSFNDD19 pKa = 4.24 CEE21 pKa = 5.23 DD22 pKa = 3.71 EE23 pKa = 4.48 VKK25 pKa = 10.73 HH26 pKa = 6.29 DD27 pKa = 3.8 TLLTNEE33 pKa = 4.08 IKK35 pKa = 9.2 TWLITNGYY43 pKa = 9.96 DD44 pKa = 4.15 NIVLNVGMLHH54 pKa = 7.18 WLTDD58 pKa = 4.83 LINSNIDD65 pKa = 3.28 CC66 pKa = 4.93
Molecular weight: 7.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.745
IPC_protein 3.668
Toseland 3.465
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.808
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A5S9C1I0|A0A5S9C1I0_9CAUD Uncharacterized protein OS=Tenacibaculum phage PTm5 OX=2547426 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.12 NLKK5 pKa = 10.55 NIDD8 pKa = 3.4 SRR10 pKa = 11.84 KK11 pKa = 9.66 LVSSTLFDD19 pKa = 3.88 VKK21 pKa = 11.01 DD22 pKa = 3.38 GDD24 pKa = 4.1 TFHH27 pKa = 6.65 TKK29 pKa = 8.92 SMRR32 pKa = 11.84 ITGFGTTWKK41 pKa = 10.04 VIKK44 pKa = 8.88 VTPKK48 pKa = 10.62 LMEE51 pKa = 4.24 LPRR54 pKa = 11.84 INYY57 pKa = 7.78 YY58 pKa = 10.84 KK59 pKa = 10.41 NPKK62 pKa = 9.01 NKK64 pKa = 9.63 TYY66 pKa = 10.38 TDD68 pKa = 3.62 RR69 pKa = 11.84 NPKK72 pKa = 8.96 VRR74 pKa = 11.84 CNNCNRR80 pKa = 11.84 PIKK83 pKa = 10.49 QNVIDD88 pKa = 4.62 RR89 pKa = 11.84 KK90 pKa = 10.56 QITPWFCYY98 pKa = 9.21 HH99 pKa = 7.07 CYY101 pKa = 10.24 LRR103 pKa = 11.84 LVKK106 pKa = 10.4 GYY108 pKa = 11.0 LNITITRR115 pKa = 11.84 KK116 pKa = 10.34 KK117 pKa = 10.55 NGLTKK122 pKa = 10.25 TIHH125 pKa = 6.55 LPTKK129 pKa = 10.3 IEE131 pKa = 4.06 LNKK134 pKa = 10.68 KK135 pKa = 9.18 KK136 pKa = 10.27 FRR138 pKa = 11.84 RR139 pKa = 3.77
Molecular weight: 16.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.259
IPC2_protein 9.78
IPC_protein 9.926
Toseland 10.687
ProMoST 10.277
Dawson 10.774
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.389
Grimsley 10.818
Solomon 10.818
Lehninger 10.804
Nozaki 10.657
DTASelect 10.379
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.687
Patrickios 11.096
IPC_peptide 10.818
IPC2_peptide 9.121
IPC2.peptide.svr19 8.611
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
306
0
306
67355
37
1336
220.1
25.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.262 ± 0.151
0.98 ± 0.056
6.372 ± 0.111
6.458 ± 0.187
4.629 ± 0.082
4.974 ± 0.174
1.929 ± 0.069
7.783 ± 0.122
9.009 ± 0.202
8.338 ± 0.146
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.3 ± 0.068
7.253 ± 0.126
2.76 ± 0.073
3.297 ± 0.092
3.764 ± 0.075
6.969 ± 0.128
6.818 ± 0.211
6.295 ± 0.12
0.983 ± 0.044
4.828 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here