Spiroplasma endosymbiont of Danaus chrysippus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; unclassified Spiroplasma

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4WTX5|A0A6J4WTX5_9MOLU Ribonuclease 3 OS=Spiroplasma endosymbiont of Danaus chrysippus OX=2691041 GN=rnc PE=3 SV=1
MM1 pKa = 7.08QSSHH5 pKa = 6.53VSTLKK10 pKa = 10.75QNFDD14 pKa = 3.91LGSFNCDD21 pKa = 3.36DD22 pKa = 3.9MCSEE26 pKa = 4.21QIKK29 pKa = 10.51NIAWDD34 pKa = 3.62VLQSQLYY41 pKa = 10.33SEE43 pKa = 4.4TQEE46 pKa = 4.15TEE48 pKa = 3.42LDD50 pKa = 3.5KK51 pKa = 11.31LLEE54 pKa = 4.7EE55 pKa = 5.0IEE57 pKa = 4.24FEE59 pKa = 4.28YY60 pKa = 10.07MANEE64 pKa = 4.16EE65 pKa = 4.06QSSLLSSEE73 pKa = 4.23NLSSNLIFDD82 pKa = 4.47DD83 pKa = 3.7MCGEE87 pKa = 4.03QVIKK91 pKa = 10.83DD92 pKa = 3.62VLNNLEE98 pKa = 4.48LDD100 pKa = 3.77QLNLLDD106 pKa = 5.48KK107 pKa = 11.01SIEE110 pKa = 3.9QLLLSEE116 pKa = 6.0KK117 pKa = 10.11IAQQNVEE124 pKa = 4.34WNNSILFVSNLDD136 pKa = 3.46NDD138 pKa = 3.64IYY140 pKa = 11.09DD141 pKa = 4.06YY142 pKa = 11.9NSVTTLIPP150 pKa = 3.74

Molecular weight: 17.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4WU39|A0A6J4WU39_9MOLU Hypothetical protein OS=Spiroplasma endosymbiont of Danaus chrysippus OX=2691041 GN=SPIRDC1_LOCUS1587 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 8.61PTYY5 pKa = 9.87QPSKK9 pKa = 9.86RR10 pKa = 11.84KK11 pKa = 8.47HH12 pKa = 5.32QKK14 pKa = 6.68THH16 pKa = 5.1GFFARR21 pKa = 11.84MKK23 pKa = 9.52SATGINVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.11KK41 pKa = 9.68LTAA44 pKa = 4.27

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

553

0

553

142806

37

1505

258.2

29.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.401 ± 0.105

0.877 ± 0.032

4.988 ± 0.086

6.035 ± 0.116

4.822 ± 0.085

4.241 ± 0.119

1.501 ± 0.043

10.581 ± 0.113

10.136 ± 0.137

9.541 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.045

9.128 ± 0.132

2.718 ± 0.057

4.153 ± 0.131

2.697 ± 0.061

6.171 ± 0.085

5.697 ± 0.094

4.823 ± 0.087

1.339 ± 0.048

4.158 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski