Enterobacteriaceae endosymbiont of Donacia sparganii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; unclassified Enterobacteriaceae

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2FNI5|A0A6H2FNI5_9ENTR 50S ribosomal protein L16 OS=Enterobacteriaceae endosymbiont of Donacia sparganii OX=2675785 GN=rplP PE=3 SV=1
MM1 pKa = 7.46SNSITKK7 pKa = 9.73RR8 pKa = 11.84VKK10 pKa = 10.49KK11 pKa = 10.47IIIDD15 pKa = 3.32QLGIKK20 pKa = 10.08KK21 pKa = 10.41NIINSASFKK30 pKa = 10.48EE31 pKa = 4.04DD32 pKa = 3.2LGADD36 pKa = 3.4SLDD39 pKa = 3.71TIEE42 pKa = 6.02IIMSLEE48 pKa = 3.76EE49 pKa = 3.96EE50 pKa = 4.37FNIEE54 pKa = 4.71ILDD57 pKa = 3.78EE58 pKa = 4.46DD59 pKa = 4.15AEE61 pKa = 4.67KK62 pKa = 9.63ITTIQEE68 pKa = 4.18AINYY72 pKa = 8.14INNYY76 pKa = 8.8KK77 pKa = 10.2KK78 pKa = 10.75

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2FNN5|A0A6H2FNN5_9ENTR Homoserine kinase OS=Enterobacteriaceae endosymbiont of Donacia sparganii OX=2675785 GN=thrB PE=3 SV=1
MM1 pKa = 7.25ATINQLVRR9 pKa = 11.84NHH11 pKa = 6.78RR12 pKa = 11.84KK13 pKa = 9.6NKK15 pKa = 9.5IIKK18 pKa = 8.93TNVPALNSSPQKK30 pKa = 10.47RR31 pKa = 11.84GVCTKK36 pKa = 10.51VYY38 pKa = 7.04TTTPKK43 pKa = 10.53KK44 pKa = 10.26PNSALRR50 pKa = 11.84KK51 pKa = 7.38VCRR54 pKa = 11.84VRR56 pKa = 11.84LTNGFEE62 pKa = 3.98VTSYY66 pKa = 11.09ISGEE70 pKa = 3.94GHH72 pKa = 6.28NLQEE76 pKa = 4.47HH77 pKa = 5.28SVILIRR83 pKa = 11.84GGRR86 pKa = 11.84VKK88 pKa = 10.7DD89 pKa = 3.73LPGVRR94 pKa = 11.84YY95 pKa = 8.06HH96 pKa = 6.93TIRR99 pKa = 11.84GSLDD103 pKa = 3.14CTGVKK108 pKa = 9.69DD109 pKa = 4.03RR110 pKa = 11.84KK111 pKa = 9.95KK112 pKa = 10.87GRR114 pKa = 11.84SKK116 pKa = 11.2YY117 pKa = 9.62GAKK120 pKa = 9.96KK121 pKa = 10.07PKK123 pKa = 10.2

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

412

0

412

139602

29

1401

338.8

39.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.492 ± 0.102

1.078 ± 0.03

3.693 ± 0.067

4.202 ± 0.106

5.383 ± 0.115

4.967 ± 0.108

1.698 ± 0.038

14.858 ± 0.168

12.142 ± 0.151

9.996 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.819 ± 0.043

9.697 ± 0.149

2.746 ± 0.049

2.666 ± 0.05

2.812 ± 0.092

5.895 ± 0.069

4.104 ± 0.058

3.301 ± 0.085

0.838 ± 0.038

4.613 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski