Clostridium butyricum E4 str. BoNT E BL5262

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium butyricum

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4IMN1|C4IMN1_CLOBU Stage II sporulation protein E OS=Clostridium butyricum E4 str. BoNT E BL5262 OX=632245 GN=spoIIE PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 9.74ATVDD6 pKa = 3.46KK7 pKa = 9.21DD8 pKa = 3.51TCIACGLCPSICPEE22 pKa = 3.97CFEE25 pKa = 5.78IEE27 pKa = 4.27DD28 pKa = 4.06DD29 pKa = 4.07GKK31 pKa = 11.07AGFIVDD37 pKa = 4.15EE38 pKa = 4.8APSGSEE44 pKa = 4.55DD45 pKa = 3.69EE46 pKa = 4.5VKK48 pKa = 10.45EE49 pKa = 3.97AAEE52 pKa = 4.22SCPVSAISVDD62 pKa = 3.32

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4IHI9|C4IHI9_CLOBU Uncharacterized protein OS=Clostridium butyricum E4 str. BoNT E BL5262 OX=632245 GN=CLP_2720 PE=4 SV=1
MM1 pKa = 7.43NPLLNMILGSLFPGMGGMMNNPMGGMGNMGGNPLMSMLMGGLGGGMNPMMGGGMNPMMGNPMNQLTQMMGGQSNNMPNMNNMPNMNNMNMGNMNPLISMLMGGQSNNGQMAPNNMNTQTPGNNQAPMMNNNMNMGNLNPLMSMLMGNRR149 pKa = 11.84NMGMNNTPMRR159 pKa = 11.84NNRR162 pKa = 11.84MMNNNQNIPQRR173 pKa = 11.84KK174 pKa = 7.52RR175 pKa = 3.06

Molecular weight: 18.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4245

0

4245

1321973

33

3156

311.4

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.415 ± 0.044

1.347 ± 0.017

5.841 ± 0.029

7.473 ± 0.043

4.334 ± 0.026

6.345 ± 0.037

1.325 ± 0.015

9.99 ± 0.049

8.924 ± 0.035

8.642 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.685 ± 0.018

6.738 ± 0.041

2.602 ± 0.02

2.284 ± 0.019

3.147 ± 0.025

6.596 ± 0.034

4.956 ± 0.032

6.321 ± 0.029

0.726 ± 0.012

4.311 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski