Enterobacteria phage M (Escherichia levivirus M)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Empivirus; Empivirus allolyticum

Average proteome isoelectric point is 8.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7QKZ0|K7QKZ0_BPM RNA replicase beta chain OS=Enterobacteria phage M OX=1235640 GN=rep PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.56YY3 pKa = 10.13IINLAFCVLLLVAGDD18 pKa = 4.26SIAYY22 pKa = 9.29RR23 pKa = 11.84VSQYY27 pKa = 10.73LAPLVDD33 pKa = 3.99TFTKK37 pKa = 10.7

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7QLN7|K7QLN7_BPM Maturation protein OS=Enterobacteria phage M OX=1235640 GN=mat PE=4 SV=1
MM1 pKa = 6.87SRR3 pKa = 11.84KK4 pKa = 9.38RR5 pKa = 11.84RR6 pKa = 11.84VTISTSQFHH15 pKa = 5.88VQLDD19 pKa = 4.18EE20 pKa = 3.91QWVLRR25 pKa = 11.84RR26 pKa = 11.84ISEE29 pKa = 4.24SLDD32 pKa = 3.15LSDD35 pKa = 5.29FRR37 pKa = 11.84NSYY40 pKa = 9.6LVKK43 pKa = 10.67SLLSKK48 pKa = 10.48YY49 pKa = 9.6AAPSKK54 pKa = 10.04EE55 pKa = 4.09SARR58 pKa = 11.84ARR60 pKa = 11.84QRR62 pKa = 11.84AAIAKK67 pKa = 8.09LLWTSRR73 pKa = 11.84RR74 pKa = 11.84NRR76 pKa = 11.84DD77 pKa = 2.75TEE79 pKa = 3.88YY80 pKa = 11.05RR81 pKa = 11.84LLRR84 pKa = 11.84RR85 pKa = 11.84DD86 pKa = 3.41SKK88 pKa = 11.23QDD90 pKa = 3.18FMFLLTRR97 pKa = 11.84EE98 pKa = 4.26VANLLGDD105 pKa = 3.62VPEE108 pKa = 4.5FEE110 pKa = 4.84GLSSFSGGASTRR122 pKa = 11.84HH123 pKa = 5.48RR124 pKa = 11.84RR125 pKa = 11.84GKK127 pKa = 9.58SQPQEE132 pKa = 4.04KK133 pKa = 10.02FDD135 pKa = 4.31GYY137 pKa = 11.73GDD139 pKa = 3.71TTASNLPLARR149 pKa = 11.84KK150 pKa = 9.85SILASPAWSCNSAVSTGGTLEE171 pKa = 4.07LRR173 pKa = 11.84LVPGNVVFTVPKK185 pKa = 10.54SNVIDD190 pKa = 3.47RR191 pKa = 11.84AAAKK195 pKa = 10.45EE196 pKa = 4.13PDD198 pKa = 3.31LNMYY202 pKa = 7.27VQKK205 pKa = 10.79FYY207 pKa = 11.33GDD209 pKa = 4.32HH210 pKa = 5.19IRR212 pKa = 11.84GRR214 pKa = 11.84LRR216 pKa = 11.84RR217 pKa = 11.84FGIDD221 pKa = 3.91LNDD224 pKa = 3.33QGRR227 pKa = 11.84NRR229 pKa = 11.84DD230 pKa = 3.47LARR233 pKa = 11.84QGSITGDD240 pKa = 3.6LATLDD245 pKa = 4.36LSSASDD251 pKa = 3.8SITRR255 pKa = 11.84ILMLEE260 pKa = 4.13VLPPAWFDD268 pKa = 3.35VLDD271 pKa = 3.97RR272 pKa = 11.84ARR274 pKa = 11.84SRR276 pKa = 11.84WTNVNGVYY284 pKa = 9.96RR285 pKa = 11.84QLHH288 pKa = 5.45MFSTMGNGFTFEE300 pKa = 4.6LEE302 pKa = 4.07SLLFFAVTRR311 pKa = 11.84VTLKK315 pKa = 9.86MLNIQGTIGIYY326 pKa = 10.53GDD328 pKa = 5.78DD329 pKa = 4.27IICPTSAARR338 pKa = 11.84EE339 pKa = 4.14VVSNLEE345 pKa = 4.08YY346 pKa = 10.49IGFKK350 pKa = 10.52TNIDD354 pKa = 3.1KK355 pKa = 11.34SFIEE359 pKa = 3.96GHH361 pKa = 5.42FRR363 pKa = 11.84EE364 pKa = 4.79SCGGHH369 pKa = 5.43YY370 pKa = 10.62YY371 pKa = 10.52KK372 pKa = 11.01GRR374 pKa = 11.84DD375 pKa = 3.23VTPFYY380 pKa = 10.68IRR382 pKa = 11.84SPLNNIQRR390 pKa = 11.84IIWLLNKK397 pKa = 9.28IRR399 pKa = 11.84EE400 pKa = 4.23WSSEE404 pKa = 3.64GTTIAVEE411 pKa = 3.98LEE413 pKa = 3.73EE414 pKa = 5.71LYY416 pKa = 11.12FEE418 pKa = 5.11IYY420 pKa = 10.77NKK422 pKa = 10.2FSILRR427 pKa = 11.84SLTGCGGLDD436 pKa = 3.84SISSLAIPGAFGGYY450 pKa = 8.35LHH452 pKa = 6.46QVTRR456 pKa = 11.84RR457 pKa = 11.84VAVNEE462 pKa = 4.04DD463 pKa = 3.08GAYY466 pKa = 9.92VQALLKK472 pKa = 10.6GSPSDD477 pKa = 3.24TLRR480 pKa = 11.84FVSVEE485 pKa = 3.59TSRR488 pKa = 11.84YY489 pKa = 9.44EE490 pKa = 3.5IRR492 pKa = 11.84QWSDD496 pKa = 2.32KK497 pKa = 10.28VRR499 pKa = 11.84PDD501 pKa = 3.22MCRR504 pKa = 11.84SLYY507 pKa = 10.78SNGRR511 pKa = 11.84MVYY514 pKa = 9.6FLRR517 pKa = 11.84EE518 pKa = 3.73IGG520 pKa = 3.46

Molecular weight: 58.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1085

37

520

271.3

30.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.912 ± 0.722

1.014 ± 0.216

4.885 ± 0.525

4.24 ± 0.767

4.7 ± 0.167

7.373 ± 0.639

1.382 ± 0.443

5.53 ± 0.32

4.055 ± 0.522

9.862 ± 0.742

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.164

4.24 ± 0.259

4.424 ± 0.868

2.673 ± 0.599

7.926 ± 1.307

10.138 ± 0.903

6.82 ± 0.753

6.636 ± 1.467

1.475 ± 0.448

3.871 ± 0.458
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski