Vavraia culicis (isolate floridensis) (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Pansporoblastina; Pleistophoridae; Vavraia; Vavraia culicis

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2768 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L2GQR3|L2GQR3_VAVCU Uncharacterized protein OS=Vavraia culicis (isolate floridensis) OX=948595 GN=VCUG_02519 PE=3 SV=1
MM1 pKa = 7.07QLDD4 pKa = 3.68VMTTDD9 pKa = 2.82KK10 pKa = 10.85RR11 pKa = 11.84LYY13 pKa = 9.05TIPEE17 pKa = 4.1KK18 pKa = 10.35MVNDD22 pKa = 5.12CILFRR27 pKa = 11.84DD28 pKa = 4.02IVNMTDD34 pKa = 2.61QCSPINILMHH44 pKa = 6.12SSVLEE49 pKa = 4.32FIMGFSEE56 pKa = 4.24INTINLPEE64 pKa = 4.96NYY66 pKa = 9.21DD67 pKa = 3.11ATQIVFPQAVFDD79 pKa = 5.37FINSLDD85 pKa = 3.85FPTLNDD91 pKa = 2.65ICTAANYY98 pKa = 10.15LYY100 pKa = 10.86YY101 pKa = 9.98PALLEE106 pKa = 4.23LCCKK110 pKa = 10.44VISKK114 pKa = 10.31KK115 pKa = 10.65LRR117 pKa = 11.84DD118 pKa = 3.49IEE120 pKa = 4.49VVSGEE125 pKa = 4.33TANEE129 pKa = 4.03LTWISSDD136 pKa = 3.37EE137 pKa = 4.15DD138 pKa = 3.6SS139 pKa = 4.31

Molecular weight: 15.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L2GYJ4|L2GYJ4_VAVCU Uncharacterized protein OS=Vavraia culicis (isolate floridensis) OX=948595 GN=VCUG_00408 PE=4 SV=1
MM1 pKa = 7.5PMKK4 pKa = 10.21IFSLHH9 pKa = 6.11RR10 pKa = 11.84FIATVHH16 pKa = 5.98HH17 pKa = 6.84KK18 pKa = 10.37GLKK21 pKa = 9.04NEE23 pKa = 4.01AMLLTQLYY31 pKa = 9.94RR32 pKa = 11.84SFLTPHH38 pKa = 6.52ARR40 pKa = 11.84ACTVVLPMVILTGNTSCLKK59 pKa = 10.51CSTVPHH65 pKa = 5.56TVSRR69 pKa = 11.84SKK71 pKa = 10.92YY72 pKa = 8.88FKK74 pKa = 10.64GLTRR78 pKa = 11.84PRR80 pKa = 11.84SGLPRR85 pKa = 11.84HH86 pKa = 5.45CQGRR90 pKa = 11.84EE91 pKa = 3.23RR92 pKa = 11.84TYY94 pKa = 10.96FSTNGTSIHH103 pKa = 6.77LLVQQHH109 pKa = 6.3DD110 pKa = 4.22LCSTAINALTQTMRR124 pKa = 11.84SALFFRR130 pKa = 11.84FSGTFVNLFCC140 pKa = 6.37

Molecular weight: 15.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2768

0

2768

952872

34

3012

344.2

39.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.745 ± 0.04

2.238 ± 0.023

5.819 ± 0.039

7.187 ± 0.051

4.84 ± 0.04

4.999 ± 0.05

2.291 ± 0.022

6.91 ± 0.04

8.115 ± 0.056

9.171 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.022

6.151 ± 0.039

2.746 ± 0.032

2.83 ± 0.023

5.473 ± 0.036

7.072 ± 0.046

5.174 ± 0.037

6.771 ± 0.043

0.586 ± 0.009

4.039 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski