Clostridium sp. CAG:628

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1091 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7MFX6|R7MFX6_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:628 OX=1262829 GN=BN740_01045 PE=4 SV=1
MM1 pKa = 7.56NDD3 pKa = 3.54DD4 pKa = 3.5EE5 pKa = 5.08KK6 pKa = 11.38KK7 pKa = 10.41IIEE10 pKa = 4.14EE11 pKa = 3.75EE12 pKa = 3.79GLYY15 pKa = 10.31FVEE18 pKa = 5.8PEE20 pKa = 4.02DD21 pKa = 3.71KK22 pKa = 10.4KK23 pKa = 10.82KK24 pKa = 10.67KK25 pKa = 9.7RR26 pKa = 11.84VIIIILLLILLLIAIGSASFSVYY49 pKa = 10.87NYY51 pKa = 9.72FKK53 pKa = 10.4IYY55 pKa = 10.22KK56 pKa = 9.3HH57 pKa = 6.33RR58 pKa = 11.84LNIDD62 pKa = 3.46TDD64 pKa = 3.26WDD66 pKa = 4.52GIADD70 pKa = 4.56LNVDD74 pKa = 4.48LDD76 pKa = 4.0NDD78 pKa = 4.21GKK80 pKa = 11.32CDD82 pKa = 3.88VNCDD86 pKa = 3.15TSGNGKK92 pKa = 9.01PDD94 pKa = 3.72LNVGYY99 pKa = 10.48KK100 pKa = 9.96KK101 pKa = 10.56VLKK104 pKa = 10.08PYY106 pKa = 10.61FNIDD110 pKa = 2.9TDD112 pKa = 4.48GDD114 pKa = 4.0GKK116 pKa = 10.02PDD118 pKa = 3.38KK119 pKa = 11.07NLINVKK125 pKa = 10.66DD126 pKa = 3.96EE127 pKa = 4.52DD128 pKa = 4.04GKK130 pKa = 11.24CIKK133 pKa = 10.86NCDD136 pKa = 4.03TNNDD140 pKa = 3.98GYY142 pKa = 10.06PDD144 pKa = 3.84TNIDD148 pKa = 3.68FDD150 pKa = 5.9DD151 pKa = 6.34DD152 pKa = 5.06GICDD156 pKa = 4.74LNCDD160 pKa = 3.31TDD162 pKa = 4.79KK163 pKa = 11.57DD164 pKa = 4.29GKK166 pKa = 10.95CDD168 pKa = 3.65VNCDD172 pKa = 3.34VNGDD176 pKa = 3.99GKK178 pKa = 11.23CDD180 pKa = 4.1LNCDD184 pKa = 3.4TDD186 pKa = 4.8KK187 pKa = 11.48DD188 pKa = 4.22GKK190 pKa = 10.0PDD192 pKa = 3.59TNLDD196 pKa = 3.43EE197 pKa = 5.82DD198 pKa = 4.84KK199 pKa = 11.52DD200 pKa = 4.46GICDD204 pKa = 3.67KK205 pKa = 11.32NCTGKK210 pKa = 10.78EE211 pKa = 3.7NDD213 pKa = 3.43VCKK216 pKa = 10.64INCDD220 pKa = 3.4TNGDD224 pKa = 3.94GKK226 pKa = 10.8CDD228 pKa = 4.35RR229 pKa = 11.84NCDD232 pKa = 3.83DD233 pKa = 5.23NKK235 pKa = 11.36DD236 pKa = 3.88GVCDD240 pKa = 4.07RR241 pKa = 11.84NCDD244 pKa = 3.3VDD246 pKa = 3.81GDD248 pKa = 4.84GYY250 pKa = 11.58CEE252 pKa = 4.53LNCDD256 pKa = 3.33TKK258 pKa = 11.74GNGKK262 pKa = 9.48CDD264 pKa = 3.87KK265 pKa = 11.32NCDD268 pKa = 3.4TDD270 pKa = 4.43NDD272 pKa = 5.1GICNVKK278 pKa = 10.32CGNTGKK284 pKa = 10.21DD285 pKa = 3.29LCLVNCDD292 pKa = 3.13TDD294 pKa = 5.02GDD296 pKa = 4.41GKK298 pKa = 10.83CDD300 pKa = 4.28KK301 pKa = 11.1NCDD304 pKa = 3.57YY305 pKa = 11.49NGDD308 pKa = 3.9GKK310 pKa = 11.24CDD312 pKa = 4.17LNCDD316 pKa = 3.55TNNNGKK322 pKa = 9.53CDD324 pKa = 4.0KK325 pKa = 11.35NCDD328 pKa = 3.67TNNDD332 pKa = 4.33GICDD336 pKa = 3.92KK337 pKa = 11.23KK338 pKa = 11.18CDD340 pKa = 3.78GNEE343 pKa = 3.87NDD345 pKa = 4.14SCKK348 pKa = 10.65LNCDD352 pKa = 3.57FNGDD356 pKa = 3.81GKK358 pKa = 10.9CDD360 pKa = 3.88KK361 pKa = 11.33NCDD364 pKa = 3.2TDD366 pKa = 5.36GDD368 pKa = 4.39GKK370 pKa = 10.78CDD372 pKa = 3.88KK373 pKa = 11.04NCDD376 pKa = 3.45VNGDD380 pKa = 3.83GKK382 pKa = 10.95CDD384 pKa = 3.77YY385 pKa = 11.4NCTEE389 pKa = 4.36NNDD392 pKa = 3.56VCLKK396 pKa = 10.77NCDD399 pKa = 3.76LNGDD403 pKa = 4.37GKK405 pKa = 11.25CDD407 pKa = 4.46LNCDD411 pKa = 3.3TDD413 pKa = 4.67NDD415 pKa = 4.31GKK417 pKa = 10.88CDD419 pKa = 4.04KK420 pKa = 11.41NCDD423 pKa = 3.4TDD425 pKa = 4.37NDD427 pKa = 5.23GICDD431 pKa = 3.83KK432 pKa = 11.48NCDD435 pKa = 3.39GKK437 pKa = 11.46EE438 pKa = 3.86NDD440 pKa = 3.48VCKK443 pKa = 10.64INCDD447 pKa = 3.4TNGDD451 pKa = 3.94GKK453 pKa = 10.79CDD455 pKa = 3.91RR456 pKa = 11.84NCDD459 pKa = 3.17TDD461 pKa = 4.92NDD463 pKa = 4.37GKK465 pKa = 10.94CDD467 pKa = 3.91KK468 pKa = 11.22NCDD471 pKa = 3.45ANGDD475 pKa = 3.88GKK477 pKa = 11.02CDD479 pKa = 3.81YY480 pKa = 11.06NCDD483 pKa = 3.83AGSDD487 pKa = 3.48KK488 pKa = 10.98CKK490 pKa = 10.51RR491 pKa = 11.84NCDD494 pKa = 3.21ADD496 pKa = 4.13NDD498 pKa = 4.53GKK500 pKa = 11.33CEE502 pKa = 4.47LNCDD506 pKa = 3.78TNNDD510 pKa = 3.66GVCDD514 pKa = 4.39KK515 pKa = 11.53NCDD518 pKa = 3.36TDD520 pKa = 4.71GDD522 pKa = 4.67GICDD526 pKa = 3.73KK527 pKa = 11.45NCDD530 pKa = 3.36GKK532 pKa = 11.49EE533 pKa = 3.83NDD535 pKa = 3.64TCKK538 pKa = 10.48INCDD542 pKa = 3.11TDD544 pKa = 4.13NDD546 pKa = 4.32GKK548 pKa = 10.88CDD550 pKa = 4.31KK551 pKa = 11.41NCDD554 pKa = 3.65TNNDD558 pKa = 3.62GVCDD562 pKa = 4.39KK563 pKa = 11.32NCDD566 pKa = 3.64SNGDD570 pKa = 3.75GKK572 pKa = 11.26CDD574 pKa = 4.12LNCDD578 pKa = 2.88IDD580 pKa = 5.59GDD582 pKa = 4.39GKK584 pKa = 10.88CDD586 pKa = 4.33KK587 pKa = 11.31NCDD590 pKa = 3.65TNGDD594 pKa = 4.35GICDD598 pKa = 4.05KK599 pKa = 11.29NCGGPVDD606 pKa = 3.3IGKK609 pKa = 9.54NDD611 pKa = 3.49EE612 pKa = 4.58YY613 pKa = 10.98ILTFKK618 pKa = 11.01DD619 pKa = 2.85ITDD622 pKa = 4.13LKK624 pKa = 10.78NVNIYY629 pKa = 9.88PGWSASKK636 pKa = 10.67VFEE639 pKa = 4.2ITNTSAVTLTYY650 pKa = 10.55NINFTSVKK658 pKa = 10.8NEE660 pKa = 3.44FSTTNNLYY668 pKa = 10.81YY669 pKa = 11.02GLIKK673 pKa = 10.57DD674 pKa = 4.1NKK676 pKa = 8.56TLIDD680 pKa = 3.8INTARR685 pKa = 11.84APYY688 pKa = 9.89KK689 pKa = 10.57DD690 pKa = 3.24GVMLKK695 pKa = 10.51SIIIKK700 pKa = 10.04PGEE703 pKa = 3.78THH705 pKa = 6.84TYY707 pKa = 8.32MFKK710 pKa = 11.11LMFKK714 pKa = 10.66DD715 pKa = 3.13SDD717 pKa = 3.83VNQDD721 pKa = 2.47IDD723 pKa = 3.53KK724 pKa = 11.24GKK726 pKa = 10.18NFYY729 pKa = 10.42TKK731 pKa = 9.65IQIEE735 pKa = 4.35NVHH738 pKa = 6.02

Molecular weight: 80.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7MIU9|R7MIU9_9CLOT Peptide chain release factor 1 OS=Clostridium sp. CAG:628 OX=1262829 GN=prfA PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 8.92LTPVAKK8 pKa = 10.37KK9 pKa = 10.23NNKK12 pKa = 9.04AKK14 pKa = 10.57KK15 pKa = 9.62QGFLSRR21 pKa = 11.84SANILKK27 pKa = 10.2SRR29 pKa = 11.84RR30 pKa = 11.84QKK32 pKa = 10.44GRR34 pKa = 11.84KK35 pKa = 8.53NLINKK40 pKa = 8.51

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1091

0

1091

319958

29

2658

293.3

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.575 ± 0.049

1.055 ± 0.036

5.767 ± 0.069

7.315 ± 0.09

4.281 ± 0.055

5.294 ± 0.087

1.141 ± 0.026

9.73 ± 0.082

10.114 ± 0.095

9.477 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.036

7.884 ± 0.093

2.323 ± 0.036

1.534 ± 0.029

2.967 ± 0.051

6.949 ± 0.077

5.762 ± 0.085

6.506 ± 0.072

0.512 ± 0.02

5.426 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski