Amino acid dipepetide frequency for Clostridium phage phiCT19406A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.87AlaAla: 1.87 ± 0.404
0.36AlaCys: 0.36 ± 0.175
2.518AlaAsp: 2.518 ± 0.407
4.244AlaGlu: 4.244 ± 0.63
1.655AlaPhe: 1.655 ± 0.323
2.59AlaGly: 2.59 ± 0.552
0.575AlaHis: 0.575 ± 0.219
4.1AlaIle: 4.1 ± 0.605
4.748AlaLys: 4.748 ± 0.719
4.388AlaLeu: 4.388 ± 0.616
1.367AlaMet: 1.367 ± 0.317
3.165AlaAsn: 3.165 ± 0.501
0.791AlaPro: 0.791 ± 0.217
1.367AlaGln: 1.367 ± 0.304
0.935AlaArg: 0.935 ± 0.29
2.518AlaSer: 2.518 ± 0.442
3.021AlaThr: 3.021 ± 0.484
1.798AlaVal: 1.798 ± 0.355
0.504AlaTrp: 0.504 ± 0.167
1.655AlaTyr: 1.655 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
0.144CysAla: 0.144 ± 0.099
0.216CysCys: 0.216 ± 0.129
0.575CysAsp: 0.575 ± 0.204
1.295CysGlu: 1.295 ± 0.473
0.432CysPhe: 0.432 ± 0.165
0.432CysGly: 0.432 ± 0.175
0.144CysHis: 0.144 ± 0.09
1.367CysIle: 1.367 ± 0.377
1.511CysLys: 1.511 ± 0.353
0.432CysLeu: 0.432 ± 0.145
0.504CysMet: 0.504 ± 0.17
0.719CysAsn: 0.719 ± 0.292
0.144CysPro: 0.144 ± 0.105
0.072CysGln: 0.072 ± 0.076
0.288CysArg: 0.288 ± 0.139
1.007CysSer: 1.007 ± 0.304
0.575CysThr: 0.575 ± 0.21
0.432CysVal: 0.432 ± 0.162
0.144CysTrp: 0.144 ± 0.088
0.647CysTyr: 0.647 ± 0.243
0.0CysXaa: 0.0 ± 0.0
Asp
1.439AspAla: 1.439 ± 0.364
0.575AspCys: 0.575 ± 0.168
2.518AspAsp: 2.518 ± 0.508
4.316AspGlu: 4.316 ± 0.536
3.309AspPhe: 3.309 ± 0.572
5.179AspGly: 5.179 ± 0.669
0.504AspHis: 0.504 ± 0.197
7.481AspIle: 7.481 ± 0.788
7.481AspLys: 7.481 ± 1.103
6.043AspLeu: 6.043 ± 0.578
1.439AspMet: 1.439 ± 0.373
4.028AspAsn: 4.028 ± 0.569
0.935AspPro: 0.935 ± 0.208
1.439AspGln: 1.439 ± 0.323
2.014AspArg: 2.014 ± 0.444
2.59AspSer: 2.59 ± 0.403
3.453AspThr: 3.453 ± 0.521
3.669AspVal: 3.669 ± 0.535
1.007AspTrp: 1.007 ± 0.249
3.813AspTyr: 3.813 ± 0.645
0.0AspXaa: 0.0 ± 0.0
Glu
3.093GluAla: 3.093 ± 0.504
1.223GluCys: 1.223 ± 0.35
4.82GluAsp: 4.82 ± 0.558
7.481GluGlu: 7.481 ± 0.739
3.165GluPhe: 3.165 ± 0.45
3.813GluGly: 3.813 ± 0.437
1.87GluHis: 1.87 ± 0.354
7.697GluIle: 7.697 ± 0.861
10.431GluLys: 10.431 ± 1.103
9.136GluLeu: 9.136 ± 0.836
2.302GluMet: 2.302 ± 0.399
5.395GluAsn: 5.395 ± 0.619
1.655GluPro: 1.655 ± 0.341
2.302GluGln: 2.302 ± 0.38
3.237GluArg: 3.237 ± 0.437
3.453GluSer: 3.453 ± 0.462
3.885GluThr: 3.885 ± 0.495
4.892GluVal: 4.892 ± 0.652
1.151GluTrp: 1.151 ± 0.235
4.1GluTyr: 4.1 ± 0.566
0.0GluXaa: 0.0 ± 0.0
Phe
1.007PheAla: 1.007 ± 0.231
0.36PheCys: 0.36 ± 0.167
2.877PheAsp: 2.877 ± 0.497
3.381PheGlu: 3.381 ± 0.494
1.295PhePhe: 1.295 ± 0.303
1.798PheGly: 1.798 ± 0.298
0.432PheHis: 0.432 ± 0.176
3.597PheIle: 3.597 ± 0.473
5.827PheLys: 5.827 ± 0.492
3.021PheLeu: 3.021 ± 0.435
0.791PheMet: 0.791 ± 0.206
3.957PheAsn: 3.957 ± 0.514
0.504PhePro: 0.504 ± 0.169
0.791PheGln: 0.791 ± 0.217
1.439PheArg: 1.439 ± 0.25
1.583PheSer: 1.583 ± 0.302
1.798PheThr: 1.798 ± 0.361
1.223PheVal: 1.223 ± 0.32
0.288PheTrp: 0.288 ± 0.134
2.014PheTyr: 2.014 ± 0.402
0.0PheXaa: 0.0 ± 0.0
Gly
2.59GlyAla: 2.59 ± 0.619
0.432GlyCys: 0.432 ± 0.156
2.302GlyAsp: 2.302 ± 0.399
4.172GlyGlu: 4.172 ± 0.506
1.87GlyPhe: 1.87 ± 0.657
2.949GlyGly: 2.949 ± 0.521
0.504GlyHis: 0.504 ± 0.157
5.467GlyIle: 5.467 ± 0.649
6.906GlyLys: 6.906 ± 0.887
4.1GlyLeu: 4.1 ± 0.649
1.511GlyMet: 1.511 ± 0.348
4.532GlyAsn: 4.532 ± 0.682
0.575GlyPro: 0.575 ± 0.194
1.942GlyGln: 1.942 ± 0.369
1.511GlyArg: 1.511 ± 0.43
2.518GlySer: 2.518 ± 0.449
3.237GlyThr: 3.237 ± 0.507
3.093GlyVal: 3.093 ± 0.462
1.079GlyTrp: 1.079 ± 0.308
2.949GlyTyr: 2.949 ± 0.579
0.0GlyXaa: 0.0 ± 0.0
His
0.647HisAla: 0.647 ± 0.212
0.144HisCys: 0.144 ± 0.096
0.935HisAsp: 0.935 ± 0.291
1.079HisGlu: 1.079 ± 0.297
0.719HisPhe: 0.719 ± 0.228
0.504HisGly: 0.504 ± 0.179
0.072HisHis: 0.072 ± 0.071
0.719HisIle: 0.719 ± 0.201
1.798HisLys: 1.798 ± 0.385
1.079HisLeu: 1.079 ± 0.264
0.36HisMet: 0.36 ± 0.147
0.647HisAsn: 0.647 ± 0.224
0.36HisPro: 0.36 ± 0.19
0.216HisGln: 0.216 ± 0.128
1.079HisArg: 1.079 ± 0.238
0.575HisSer: 0.575 ± 0.279
0.719HisThr: 0.719 ± 0.225
0.575HisVal: 0.575 ± 0.177
0.647HisTrp: 0.647 ± 0.187
0.432HisTyr: 0.432 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
3.453IleAla: 3.453 ± 0.399
1.079IleCys: 1.079 ± 0.306
6.546IleAsp: 6.546 ± 0.864
8.92IleGlu: 8.92 ± 0.905
2.23IlePhe: 2.23 ± 0.339
4.964IleGly: 4.964 ± 0.641
1.151IleHis: 1.151 ± 0.244
6.474IleIle: 6.474 ± 0.867
11.87IleLys: 11.87 ± 1.095
7.338IleLeu: 7.338 ± 0.758
1.367IleMet: 1.367 ± 0.382
8.489IleAsn: 8.489 ± 0.921
2.59IlePro: 2.59 ± 0.426
2.59IleGln: 2.59 ± 0.403
3.669IleArg: 3.669 ± 0.44
4.604IleSer: 4.604 ± 0.59
4.46IleThr: 4.46 ± 0.484
4.1IleVal: 4.1 ± 0.503
0.575IleTrp: 0.575 ± 0.182
4.244IleTyr: 4.244 ± 0.587
0.0IleXaa: 0.0 ± 0.0
Lys
4.964LysAla: 4.964 ± 0.578
1.439LysCys: 1.439 ± 0.458
8.776LysAsp: 8.776 ± 1.111
12.229LysGlu: 12.229 ± 1.17
3.885LysPhe: 3.885 ± 0.541
5.755LysGly: 5.755 ± 0.725
1.942LysHis: 1.942 ± 0.328
9.064LysIle: 9.064 ± 0.884
11.366LysLys: 11.366 ± 1.025
9.64LysLeu: 9.64 ± 0.82
3.813LysMet: 3.813 ± 0.511
7.05LysAsn: 7.05 ± 0.553
2.877LysPro: 2.877 ± 0.482
4.604LysGln: 4.604 ± 0.551
3.813LysArg: 3.813 ± 0.553
6.69LysSer: 6.69 ± 0.701
5.467LysThr: 5.467 ± 0.62
5.971LysVal: 5.971 ± 0.541
1.295LysTrp: 1.295 ± 0.265
6.115LysTyr: 6.115 ± 1.073
0.0LysXaa: 0.0 ± 0.0
Leu
4.172LeuAla: 4.172 ± 0.68
1.079LeuCys: 1.079 ± 0.276
5.683LeuAsp: 5.683 ± 0.695
8.201LeuGlu: 8.201 ± 0.817
3.381LeuPhe: 3.381 ± 0.39
5.036LeuGly: 5.036 ± 0.934
1.079LeuHis: 1.079 ± 0.367
6.043LeuIle: 6.043 ± 0.555
11.654LeuLys: 11.654 ± 0.942
6.043LeuLeu: 6.043 ± 0.908
1.87LeuMet: 1.87 ± 0.338
6.259LeuAsn: 6.259 ± 0.711
1.726LeuPro: 1.726 ± 0.392
2.734LeuGln: 2.734 ± 0.472
2.518LeuArg: 2.518 ± 0.476
4.388LeuSer: 4.388 ± 0.671
3.813LeuThr: 3.813 ± 0.661
4.316LeuVal: 4.316 ± 0.603
0.647LeuTrp: 0.647 ± 0.227
3.165LeuTyr: 3.165 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
1.942MetAla: 1.942 ± 0.583
0.144MetCys: 0.144 ± 0.107
1.583MetAsp: 1.583 ± 0.286
2.59MetGlu: 2.59 ± 0.392
0.791MetPhe: 0.791 ± 0.245
0.935MetGly: 0.935 ± 0.346
0.216MetHis: 0.216 ± 0.122
1.439MetIle: 1.439 ± 0.287
2.662MetLys: 2.662 ± 0.443
2.014MetLeu: 2.014 ± 0.375
0.719MetMet: 0.719 ± 0.226
1.87MetAsn: 1.87 ± 0.398
0.935MetPro: 0.935 ± 0.308
1.367MetGln: 1.367 ± 0.359
0.791MetArg: 0.791 ± 0.191
2.158MetSer: 2.158 ± 0.359
0.863MetThr: 0.863 ± 0.238
1.079MetVal: 1.079 ± 0.279
0.432MetTrp: 0.432 ± 0.189
1.367MetTyr: 1.367 ± 0.35
0.0MetXaa: 0.0 ± 0.0
Asn
2.949AsnAla: 2.949 ± 0.356
0.647AsnCys: 0.647 ± 0.264
3.957AsnAsp: 3.957 ± 0.485
4.46AsnGlu: 4.46 ± 0.615
3.813AsnPhe: 3.813 ± 0.495
3.309AsnGly: 3.309 ± 0.456
0.575AsnHis: 0.575 ± 0.235
8.201AsnIle: 8.201 ± 0.895
8.561AsnLys: 8.561 ± 0.942
6.402AsnLeu: 6.402 ± 0.674
1.726AsnMet: 1.726 ± 0.392
5.899AsnAsn: 5.899 ± 0.821
2.806AsnPro: 2.806 ± 0.549
1.655AsnGln: 1.655 ± 0.411
2.949AsnArg: 2.949 ± 0.373
4.316AsnSer: 4.316 ± 0.662
3.381AsnThr: 3.381 ± 0.45
3.021AsnVal: 3.021 ± 0.457
0.863AsnTrp: 0.863 ± 0.241
3.669AsnTyr: 3.669 ± 0.521
0.0AsnXaa: 0.0 ± 0.0
Pro
1.151ProAla: 1.151 ± 0.265
0.216ProCys: 0.216 ± 0.11
1.439ProAsp: 1.439 ± 0.307
2.086ProGlu: 2.086 ± 0.362
1.367ProPhe: 1.367 ± 0.318
0.863ProGly: 0.863 ± 0.248
0.288ProHis: 0.288 ± 0.146
2.302ProIle: 2.302 ± 0.42
1.798ProLys: 1.798 ± 0.395
1.798ProLeu: 1.798 ± 0.424
0.647ProMet: 0.647 ± 0.279
1.367ProAsn: 1.367 ± 0.296
0.863ProPro: 0.863 ± 0.361
0.719ProGln: 0.719 ± 0.209
0.719ProArg: 0.719 ± 0.248
2.014ProSer: 2.014 ± 0.35
0.935ProThr: 0.935 ± 0.241
2.086ProVal: 2.086 ± 0.398
0.144ProTrp: 0.144 ± 0.099
1.295ProTyr: 1.295 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
1.726GlnAla: 1.726 ± 0.3
0.144GlnCys: 0.144 ± 0.1
1.439GlnAsp: 1.439 ± 0.4
2.59GlnGlu: 2.59 ± 0.372
1.223GlnPhe: 1.223 ± 0.265
1.87GlnGly: 1.87 ± 0.308
0.575GlnHis: 0.575 ± 0.237
3.021GlnIle: 3.021 ± 0.437
2.662GlnLys: 2.662 ± 0.367
2.806GlnLeu: 2.806 ± 0.412
0.647GlnMet: 0.647 ± 0.171
2.302GlnAsn: 2.302 ± 0.326
0.935GlnPro: 0.935 ± 0.261
1.151GlnGln: 1.151 ± 0.293
1.223GlnArg: 1.223 ± 0.282
1.726GlnSer: 1.726 ± 0.36
1.151GlnThr: 1.151 ± 0.273
1.151GlnVal: 1.151 ± 0.255
0.36GlnTrp: 0.36 ± 0.202
1.439GlnTyr: 1.439 ± 0.27
0.0GlnXaa: 0.0 ± 0.0
Arg
1.655ArgAla: 1.655 ± 0.382
0.432ArgCys: 0.432 ± 0.165
2.662ArgAsp: 2.662 ± 0.437
2.949ArgGlu: 2.949 ± 0.471
1.151ArgPhe: 1.151 ± 0.284
1.583ArgGly: 1.583 ± 0.304
0.504ArgHis: 0.504 ± 0.177
3.309ArgIle: 3.309 ± 0.51
3.093ArgLys: 3.093 ± 0.454
2.518ArgLeu: 2.518 ± 0.474
1.223ArgMet: 1.223 ± 0.288
1.726ArgAsn: 1.726 ± 0.351
0.863ArgPro: 0.863 ± 0.213
1.367ArgGln: 1.367 ± 0.325
1.655ArgArg: 1.655 ± 0.426
1.511ArgSer: 1.511 ± 0.412
2.014ArgThr: 2.014 ± 0.344
2.23ArgVal: 2.23 ± 0.347
0.647ArgTrp: 0.647 ± 0.288
2.014ArgTyr: 2.014 ± 0.317
0.0ArgXaa: 0.0 ± 0.0
Ser
2.446SerAla: 2.446 ± 0.784
0.791SerCys: 0.791 ± 0.215
3.309SerAsp: 3.309 ± 0.467
4.028SerGlu: 4.028 ± 0.677
2.014SerPhe: 2.014 ± 0.39
3.093SerGly: 3.093 ± 0.598
0.791SerHis: 0.791 ± 0.237
5.036SerIle: 5.036 ± 0.634
6.187SerLys: 6.187 ± 0.633
4.244SerLeu: 4.244 ± 0.618
1.295SerMet: 1.295 ± 0.319
4.892SerAsn: 4.892 ± 0.748
1.007SerPro: 1.007 ± 0.218
1.151SerGln: 1.151 ± 0.276
2.446SerArg: 2.446 ± 0.375
2.374SerSer: 2.374 ± 0.394
2.086SerThr: 2.086 ± 0.308
1.942SerVal: 1.942 ± 0.344
0.575SerTrp: 0.575 ± 0.215
2.158SerTyr: 2.158 ± 0.529
0.0SerXaa: 0.0 ± 0.0
Thr
2.446ThrAla: 2.446 ± 0.514
0.288ThrCys: 0.288 ± 0.178
3.741ThrAsp: 3.741 ± 0.525
3.957ThrGlu: 3.957 ± 0.633
2.014ThrPhe: 2.014 ± 0.311
3.957ThrGly: 3.957 ± 0.459
0.575ThrHis: 0.575 ± 0.175
5.108ThrIle: 5.108 ± 0.752
4.892ThrLys: 4.892 ± 0.668
4.46ThrLeu: 4.46 ± 0.59
1.223ThrMet: 1.223 ± 0.304
3.165ThrAsn: 3.165 ± 0.389
1.655ThrPro: 1.655 ± 0.361
1.439ThrGln: 1.439 ± 0.348
0.719ThrArg: 0.719 ± 0.219
2.806ThrSer: 2.806 ± 0.409
2.877ThrThr: 2.877 ± 0.524
2.518ThrVal: 2.518 ± 0.463
0.432ThrTrp: 0.432 ± 0.162
1.511ThrTyr: 1.511 ± 0.31
0.0ThrXaa: 0.0 ± 0.0
Val
4.1ValAla: 4.1 ± 0.708
0.36ValCys: 0.36 ± 0.141
4.028ValAsp: 4.028 ± 0.473
3.309ValGlu: 3.309 ± 0.47
1.367ValPhe: 1.367 ± 0.362
1.726ValGly: 1.726 ± 0.345
0.432ValHis: 0.432 ± 0.205
4.748ValIle: 4.748 ± 0.529
5.467ValLys: 5.467 ± 0.691
4.028ValLeu: 4.028 ± 0.505
1.655ValMet: 1.655 ± 0.353
3.309ValAsn: 3.309 ± 0.474
1.439ValPro: 1.439 ± 0.275
1.655ValGln: 1.655 ± 0.395
1.87ValArg: 1.87 ± 0.335
2.014ValSer: 2.014 ± 0.361
3.021ValThr: 3.021 ± 0.525
2.877ValVal: 2.877 ± 0.498
0.647ValTrp: 0.647 ± 0.211
1.942ValTyr: 1.942 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
0.288TrpAla: 0.288 ± 0.132
0.288TrpCys: 0.288 ± 0.133
0.719TrpAsp: 0.719 ± 0.22
0.863TrpGlu: 0.863 ± 0.246
0.575TrpPhe: 0.575 ± 0.214
1.007TrpGly: 1.007 ± 0.252
0.288TrpHis: 0.288 ± 0.126
1.151TrpIle: 1.151 ± 0.293
1.295TrpLys: 1.295 ± 0.309
0.935TrpLeu: 0.935 ± 0.26
0.288TrpMet: 0.288 ± 0.132
0.863TrpAsn: 0.863 ± 0.24
0.0TrpPro: 0.0 ± 0.0
0.36TrpGln: 0.36 ± 0.146
0.432TrpArg: 0.432 ± 0.154
0.575TrpSer: 0.575 ± 0.197
0.719TrpThr: 0.719 ± 0.207
0.791TrpVal: 0.791 ± 0.185
0.288TrpTrp: 0.288 ± 0.166
0.647TrpTyr: 0.647 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.086TyrAla: 2.086 ± 0.377
0.863TyrCys: 0.863 ± 0.266
2.806TyrAsp: 2.806 ± 0.536
2.949TyrGlu: 2.949 ± 0.411
1.726TyrPhe: 1.726 ± 0.438
2.877TyrGly: 2.877 ± 0.371
0.791TyrHis: 0.791 ± 0.199
4.388TyrIle: 4.388 ± 0.502
6.618TyrLys: 6.618 ± 0.821
3.381TyrLeu: 3.381 ± 0.478
1.079TyrMet: 1.079 ± 0.263
3.525TyrAsn: 3.525 ± 0.543
1.439TyrPro: 1.439 ± 0.369
1.223TyrGln: 1.223 ± 0.316
1.798TyrArg: 1.798 ± 0.366
2.446TyrSer: 2.446 ± 0.511
2.302TyrThr: 2.302 ± 0.457
2.23TyrVal: 2.23 ± 0.347
0.647TyrTrp: 0.647 ± 0.188
2.014TyrTyr: 2.014 ± 0.421
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (13902 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski