Sus scrofa papillomavirus 1
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B5SPY3|B5SPY3_9PAPI Regulatory protein E2 OS=Sus scrofa papillomavirus 1 OX=446138 GN=E2 PE=3 SV=1
MM1 pKa = 7.71 EE2 pKa = 5.68 EE3 pKa = 4.35 GNSPLEE9 pKa = 4.46 GCSGDD14 pKa = 3.72 WILVEE19 pKa = 4.39 AEE21 pKa = 4.38 EE22 pKa = 4.64 EE23 pKa = 4.39 GGACGGDD30 pKa = 3.44 GEE32 pKa = 4.69 TDD34 pKa = 3.24 SDD36 pKa = 4.34 DD37 pKa = 4.64 GYY39 pKa = 11.42 DD40 pKa = 3.35 MVDD43 pKa = 4.99 FIDD46 pKa = 4.19 DD47 pKa = 3.92 TEE49 pKa = 4.56 VPQGNSAALYY59 pKa = 9.36 HH60 pKa = 6.46 AQTLYY65 pKa = 10.01 DD66 pKa = 3.72 TAVLPQCKK74 pKa = 8.82 RR75 pKa = 11.84 KK76 pKa = 9.56 HH77 pKa = 5.81 VNSPKK82 pKa = 10.32 VKK84 pKa = 9.39 PVCDD88 pKa = 4.3 LSPRR92 pKa = 11.84 LAAINIGTEE101 pKa = 4.04 SQQTAKK107 pKa = 10.35 RR108 pKa = 11.84 RR109 pKa = 11.84 LFVAPPDD116 pKa = 3.59 SGYY119 pKa = 11.55 GNTLEE124 pKa = 4.18 EE125 pKa = 4.97 ANTQEE130 pKa = 4.11 MGEE133 pKa = 4.35 TEE135 pKa = 4.3 VPGGNGGEE143 pKa = 4.32 GEE145 pKa = 4.34 HH146 pKa = 5.97 MVAEE150 pKa = 4.36 AFGQEE155 pKa = 3.68 RR156 pKa = 11.84 RR157 pKa = 11.84 RR158 pKa = 11.84 AAMLGQFKK166 pKa = 10.41 EE167 pKa = 4.0 RR168 pKa = 11.84 FGISFCDD175 pKa = 3.29 LTRR178 pKa = 11.84 PFKK181 pKa = 11.09 SNKK184 pKa = 5.4 TTCGDD189 pKa = 3.35 WVVYY193 pKa = 10.05 ACGMSDD199 pKa = 3.76 ALADD203 pKa = 3.72 ATSDD207 pKa = 3.93 LLSPHH212 pKa = 7.2 CIYY215 pKa = 11.37 GNITKK220 pKa = 9.76 EE221 pKa = 3.89 HH222 pKa = 6.01 SALGNSLLMLLRR234 pKa = 11.84 FTHH237 pKa = 6.58 AKK239 pKa = 10.08 NRR241 pKa = 11.84 DD242 pKa = 3.61 TLTKK246 pKa = 10.73 LLGSHH251 pKa = 5.4 MTIPAYY257 pKa = 10.67 NIVAEE262 pKa = 4.39 PPKK265 pKa = 10.18 IRR267 pKa = 11.84 CPAAAIFWYY276 pKa = 10.17 RR277 pKa = 11.84 RR278 pKa = 11.84 STTQGVTVFGDD289 pKa = 3.27 VCEE292 pKa = 4.85 WITKK296 pKa = 8.87 QVTVGGGEE304 pKa = 4.26 CAFEE308 pKa = 4.52 LSTMIQWAYY317 pKa = 11.08 DD318 pKa = 3.34 SDD320 pKa = 4.15 LTDD323 pKa = 3.65 EE324 pKa = 4.52 AQVAYY329 pKa = 9.65 EE330 pKa = 4.01 YY331 pKa = 11.57 AKK333 pKa = 10.98 LADD336 pKa = 3.55 SDD338 pKa = 4.28 RR339 pKa = 11.84 NAEE342 pKa = 4.37 AFLKK346 pKa = 10.78 SNCQAKK352 pKa = 9.52 YY353 pKa = 11.04 VKK355 pKa = 10.04 DD356 pKa = 3.62 CCTMVRR362 pKa = 11.84 LYY364 pKa = 10.93 KK365 pKa = 10.17 KK366 pKa = 10.78 AEE368 pKa = 3.9 MHH370 pKa = 6.13 NMTMGQWVRR379 pKa = 11.84 KK380 pKa = 8.63 QADD383 pKa = 3.81 RR384 pKa = 11.84 YY385 pKa = 9.6 EE386 pKa = 4.86 GEE388 pKa = 4.07 GNYY391 pKa = 10.03 RR392 pKa = 11.84 PIVQFLKK399 pKa = 10.28 FQGIEE404 pKa = 4.59 FIPFMHH410 pKa = 7.07 TLAQFLKK417 pKa = 10.65 GIPKK421 pKa = 9.96 RR422 pKa = 11.84 NCLVIHH428 pKa = 6.61 GPPNTGKK435 pKa = 10.55 SLFTMSLCSFLGGKK449 pKa = 9.53 VISFVNSKK457 pKa = 8.57 SHH459 pKa = 7.02 FWLQPLGEE467 pKa = 4.64 CKK469 pKa = 10.17 VALLDD474 pKa = 5.14 DD475 pKa = 3.97 ATRR478 pKa = 11.84 PCWDD482 pKa = 3.51 YY483 pKa = 11.68 FDD485 pKa = 6.11 LYY487 pKa = 11.09 LRR489 pKa = 11.84 NLLDD493 pKa = 4.33 GNPVSLDD500 pKa = 3.33 AKK502 pKa = 10.13 HH503 pKa = 6.26 RR504 pKa = 11.84 APIQIKK510 pKa = 10.32 GPPLLITSNVGISTEE525 pKa = 4.12 DD526 pKa = 2.67 RR527 pKa = 11.84 WKK529 pKa = 10.11 YY530 pKa = 8.2 LHH532 pKa = 6.71 SRR534 pKa = 11.84 VRR536 pKa = 11.84 CFSFPNTLPLDD547 pKa = 4.05 SKK549 pKa = 11.35 GDD551 pKa = 3.65 PVYY554 pKa = 11.01 EE555 pKa = 3.83 LTALNWKK562 pKa = 10.19 SFFTRR567 pKa = 11.84 LWSRR571 pKa = 11.84 LDD573 pKa = 3.61 LKK575 pKa = 10.85 EE576 pKa = 4.3 GSHH579 pKa = 6.46 GEE581 pKa = 3.68 AFTALRR587 pKa = 11.84 CTTGPAAGPDD597 pKa = 3.34
Molecular weight: 66.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.412
IPC2_protein 5.461
IPC_protein 5.474
Toseland 5.677
ProMoST 5.677
Dawson 5.639
Bjellqvist 5.703
Wikipedia 5.601
Rodwell 5.601
Grimsley 5.728
Solomon 5.639
Lehninger 5.614
Nozaki 5.855
DTASelect 6.033
Thurlkill 5.944
EMBOSS 5.906
Sillero 5.944
Patrickios 3.872
IPC_peptide 5.652
IPC2_peptide 5.957
IPC2.peptide.svr19 5.911
Protein with the highest isoelectric point:
>tr|B5SPY4|B5SPY4_9PAPI Minor capsid protein L2 OS=Sus scrofa papillomavirus 1 OX=446138 GN=L2 PE=3 SV=1
MM1 pKa = 7.42 EE2 pKa = 5.9 RR3 pKa = 11.84 LSQRR7 pKa = 11.84 LGALQDD13 pKa = 3.55 QQLDD17 pKa = 4.36 LIEE20 pKa = 5.58 KK21 pKa = 9.54 DD22 pKa = 3.65 SQSIGDD28 pKa = 3.72 QLYY31 pKa = 8.17 YY32 pKa = 10.19 WYY34 pKa = 10.32 LQRR37 pKa = 11.84 QEE39 pKa = 4.16 HH40 pKa = 5.52 TLLYY44 pKa = 10.01 AARR47 pKa = 11.84 KK48 pKa = 9.78 KK49 pKa = 10.87 GVTSINGQPVPTAQVSHH66 pKa = 6.26 SRR68 pKa = 11.84 ASQAIEE74 pKa = 3.62 MHH76 pKa = 6.79 LALQGLGQLYY86 pKa = 10.58 AEE88 pKa = 5.17 EE89 pKa = 4.77 PWTMLQTTWEE99 pKa = 4.59 SYY101 pKa = 10.15 RR102 pKa = 11.84 QTPSGTFKK110 pKa = 10.92 KK111 pKa = 10.02 GGLSIRR117 pKa = 11.84 VQFDD121 pKa = 3.63 GEE123 pKa = 4.4 KK124 pKa = 10.41 DD125 pKa = 3.43 NEE127 pKa = 3.98 MDD129 pKa = 3.67 YY130 pKa = 11.39 VLWDD134 pKa = 3.3 TVYY137 pKa = 11.64 YY138 pKa = 7.09 MTGDD142 pKa = 3.53 GGWRR146 pKa = 11.84 KK147 pKa = 9.96 GKK149 pKa = 10.58 GGWDD153 pKa = 3.37 NTGLYY158 pKa = 10.3 YY159 pKa = 10.95 LADD162 pKa = 4.05 GEE164 pKa = 4.36 KK165 pKa = 9.53 QHH167 pKa = 6.08 YY168 pKa = 10.78 VNFKK172 pKa = 9.94 TEE174 pKa = 3.74 AQKK177 pKa = 10.38 FAKK180 pKa = 10.31 LGIWKK185 pKa = 8.53 VLCTDD190 pKa = 3.34 SHH192 pKa = 6.86 HH193 pKa = 7.72 DD194 pKa = 3.62 SDD196 pKa = 5.53 LVTSTTTATSTSTQSLSGPCWDD218 pKa = 4.1 PHH220 pKa = 6.43 PPTTTAEE227 pKa = 4.23 STSRR231 pKa = 11.84 SPWDD235 pKa = 3.63 TPPTEE240 pKa = 3.73 TAARR244 pKa = 11.84 RR245 pKa = 11.84 RR246 pKa = 11.84 LATTATPCARR256 pKa = 11.84 RR257 pKa = 11.84 PQAVPADD264 pKa = 3.99 SPSPVPRR271 pKa = 11.84 WGPGQGKK278 pKa = 8.99 HH279 pKa = 6.27 PSTPDD284 pKa = 2.86 ATAKK288 pKa = 8.37 PTAAPAATNTTTYY301 pKa = 7.03 TTTAGRR307 pKa = 11.84 GSIGPQCLCDD317 pKa = 4.18 CDD319 pKa = 5.63 PNTTWGSEE327 pKa = 4.11 SQAGVPLTAPLVVLKK342 pKa = 10.8 GDD344 pKa = 3.92 PNQLKK349 pKa = 10.27 CYY351 pKa = 10.16 RR352 pKa = 11.84 YY353 pKa = 9.01 RR354 pKa = 11.84 LKK356 pKa = 10.54 KK357 pKa = 10.19 GRR359 pKa = 11.84 RR360 pKa = 11.84 KK361 pKa = 9.93 SYY363 pKa = 9.75 RR364 pKa = 11.84 HH365 pKa = 6.83 CSTTWQWVGCDD376 pKa = 3.28 GVSRR380 pKa = 11.84 TGKK383 pKa = 9.83 HH384 pKa = 5.55 LLCVSFDD391 pKa = 3.55 NSAQRR396 pKa = 11.84 EE397 pKa = 4.36 EE398 pKa = 4.36 FLLTAQLPQGVTATRR413 pKa = 11.84 ADD415 pKa = 3.78 FPLL418 pKa = 5.26
Molecular weight: 46.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.327
IPC2_protein 7.629
IPC_protein 7.483
Toseland 7.117
ProMoST 8.053
Dawson 8.17
Bjellqvist 8.565
Wikipedia 8.097
Rodwell 8.2
Grimsley 7.161
Solomon 8.244
Lehninger 8.258
Nozaki 8.77
DTASelect 8.287
Thurlkill 8.331
EMBOSS 8.39
Sillero 8.668
Patrickios 4.177
IPC_peptide 8.244
IPC2_peptide 7.629
IPC2.peptide.svr19 7.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2185
165
597
437.0
48.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.499 ± 0.661
2.38 ± 0.603
6.224 ± 0.166
4.805 ± 0.519
4.027 ± 0.567
8.65 ± 1.077
2.288 ± 0.057
3.432 ± 0.528
4.119 ± 0.753
8.741 ± 0.417
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.739 ± 0.29
3.341 ± 0.731
7.506 ± 1.15
4.165 ± 0.732
6.178 ± 0.595
7.048 ± 0.391
8.146 ± 0.88
5.995 ± 0.713
1.831 ± 0.413
2.883 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here