Pygoscelis adeliae (Adelie penguin)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093QYD4|A0A093QYD4_PYGAD ELM2 and SANT domain-containing protein 1 (Fragment) OS=Pygoscelis adeliae OX=9238 GN=AS28_05217 PE=4 SV=1
SS1 pKa = 6.99RR2 pKa = 11.84SGIGSRR8 pKa = 11.84SSSMASQTSSEE19 pKa = 4.27MSWVDD24 pKa = 4.49LNLPDD29 pKa = 4.92APEE32 pKa = 4.62APPCYY37 pKa = 10.04LDD39 pKa = 4.97IVPEE43 pKa = 4.0DD44 pKa = 3.92HH45 pKa = 7.18RR46 pKa = 11.84LEE48 pKa = 4.67SPTTPLLDD56 pKa = 4.28DD57 pKa = 4.88PEE59 pKa = 5.87SFDD62 pKa = 4.97SPIFMYY68 pKa = 10.71APEE71 pKa = 4.76FKK73 pKa = 10.58FMPPPTYY80 pKa = 10.51TEE82 pKa = 3.9VDD84 pKa = 3.22PCIANNNVQQ93 pKa = 3.18

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093PKX8|A0A093PKX8_PYGAD Uncharacterized protein (Fragment) OS=Pygoscelis adeliae OX=9238 GN=AS28_08622 PE=4 SV=1
KKK2 pKa = 10.12RR3 pKa = 11.84QGKKK7 pKa = 8.85RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.73RR48 pKa = 11.84QGKKK52 pKa = 8.73RR53 pKa = 11.84QGKKK57 pKa = 9.07RR58 pKa = 11.84

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12848

0

12848

5703779

31

5422

443.9

49.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.363 ± 0.019

2.257 ± 0.021

5.058 ± 0.017

7.198 ± 0.028

3.918 ± 0.016

5.883 ± 0.027

2.537 ± 0.009

5.025 ± 0.016

6.535 ± 0.026

9.685 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.009

4.186 ± 0.015

5.226 ± 0.022

4.635 ± 0.02

5.226 ± 0.018

8.166 ± 0.03

5.38 ± 0.014

6.287 ± 0.017

1.19 ± 0.007

3.004 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski