Corchorus golden mosaic virus [India:Bahraich:2008]
Average proteome isoelectric point is 8.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7UA01|B7UA01_9GEMI AC4 OS=Corchorus golden mosaic virus [India:Bahraich:2008] OX=582838 GN=AC4 PE=3 SV=1
MM1 pKa = 7.54 SGRR4 pKa = 11.84 FRR6 pKa = 11.84 KK7 pKa = 9.97 QGVSFFLTWPKK18 pKa = 10.76 CPVTKK23 pKa = 10.45 EE24 pKa = 4.03 SALDD28 pKa = 3.59 QILALALPTNIIYY41 pKa = 10.18 IRR43 pKa = 11.84 VCEE46 pKa = 4.36 EE47 pKa = 3.47 KK48 pKa = 10.64 HH49 pKa = 6.14 QDD51 pKa = 3.54 GSPHH55 pKa = 5.97 LHH57 pKa = 6.9 ALVQFQKK64 pKa = 11.17 KK65 pKa = 9.05 FICTNCRR72 pKa = 11.84 LFDD75 pKa = 4.13 LSHH78 pKa = 6.67 PQNSRR83 pKa = 11.84 QFHH86 pKa = 5.18 CHH88 pKa = 5.9 IEE90 pKa = 4.25 TARR93 pKa = 11.84 SSSDD97 pKa = 2.64 AKK99 pKa = 11.09 SYY101 pKa = 10.6 IEE103 pKa = 4.15 KK104 pKa = 10.8 DD105 pKa = 3.55 GVFCEE110 pKa = 4.16 WGTFQVDD117 pKa = 3.02 GRR119 pKa = 11.84 SARR122 pKa = 11.84 GGQQTINEE130 pKa = 4.51 AYY132 pKa = 10.29 AKK134 pKa = 10.32 ALNSGSKK141 pKa = 10.68 DD142 pKa = 3.25 EE143 pKa = 4.61 ALTVIKK149 pKa = 10.39 EE150 pKa = 4.24 LVPKK154 pKa = 10.49 DD155 pKa = 3.87 YY156 pKa = 11.34 VCQFHH161 pKa = 6.83 NLNANLEE168 pKa = 4.51 RR169 pKa = 11.84 IFAPPVHH176 pKa = 6.89 VFEE179 pKa = 5.27 PPFPLSSFNNVPAVINQWVNDD200 pKa = 4.13 NIMDD204 pKa = 4.59 AAARR208 pKa = 11.84 PFRR211 pKa = 11.84 PISIVIEE218 pKa = 4.68 GPSRR222 pKa = 11.84 TGKK225 pKa = 7.64 TLWARR230 pKa = 11.84 SLGRR234 pKa = 11.84 HH235 pKa = 5.8 NYY237 pKa = 10.07 LCGHH241 pKa = 7.41 LDD243 pKa = 4.18 LSPKK247 pKa = 10.02 VYY249 pKa = 10.89 SNEE252 pKa = 3.02 AWYY255 pKa = 10.82 NVIDD259 pKa = 5.29 DD260 pKa = 4.41 VDD262 pKa = 3.64 PHH264 pKa = 6.13 YY265 pKa = 10.52 LKK267 pKa = 10.88 HH268 pKa = 5.92 MKK270 pKa = 10.16 EE271 pKa = 4.06 FMGAQRR277 pKa = 11.84 DD278 pKa = 3.71 WQSNCKK284 pKa = 9.1 YY285 pKa = 10.44 GKK287 pKa = 9.33 PIQINGGIPTIFLCNPGPTSSYY309 pKa = 10.54 KK310 pKa = 10.57 EE311 pKa = 3.87 YY312 pKa = 10.98 FEE314 pKa = 4.61 EE315 pKa = 4.44 EE316 pKa = 4.27 KK317 pKa = 11.17 NSAIYY322 pKa = 10.38 DD323 pKa = 3.71 WAKK326 pKa = 10.67 KK327 pKa = 9.77 NAIFVTIEE335 pKa = 3.71 EE336 pKa = 4.81 PFFDD340 pKa = 4.17 TADD343 pKa = 3.54 QASTSTLEE351 pKa = 4.17 EE352 pKa = 4.69 SNSSS356 pKa = 3.27
Molecular weight: 40.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.039
IPC2_protein 6.046
IPC_protein 6.135
Toseland 6.415
ProMoST 6.517
Dawson 6.478
Bjellqvist 6.465
Wikipedia 6.504
Rodwell 6.478
Grimsley 6.532
Solomon 6.478
Lehninger 6.478
Nozaki 6.766
DTASelect 6.927
Thurlkill 6.956
EMBOSS 6.927
Sillero 6.868
Patrickios 4.012
IPC_peptide 6.504
IPC2_peptide 6.766
IPC2.peptide.svr19 6.742
Protein with the highest isoelectric point:
>tr|B7U9Z8|B7U9Z8_9GEMI Replication enhancer OS=Corchorus golden mosaic virus [India:Bahraich:2008] OX=582838 GN=AC3 PE=3 SV=1
MM1 pKa = 7.44 MKK3 pKa = 10.24 RR4 pKa = 11.84 EE5 pKa = 4.26 APWRR9 pKa = 11.84 TNAGTSKK16 pKa = 10.09 VRR18 pKa = 11.84 RR19 pKa = 11.84 SLNFSPRR26 pKa = 11.84 SGLGPKK32 pKa = 10.13 ASAWVNRR39 pKa = 11.84 PMYY42 pKa = 9.63 RR43 pKa = 11.84 KK44 pKa = 8.55 PRR46 pKa = 11.84 IYY48 pKa = 9.41 RR49 pKa = 11.84 TYY51 pKa = 10.75 RR52 pKa = 11.84 SPDD55 pKa = 3.09 VSKK58 pKa = 11.11 GCEE61 pKa = 4.44 GPCKK65 pKa = 10.23 VQSFEE70 pKa = 3.82 QRR72 pKa = 11.84 HH73 pKa = 6.26 DD74 pKa = 3.16 ISHH77 pKa = 6.13 VGKK80 pKa = 10.55 VMCISDD86 pKa = 3.56 VTRR89 pKa = 11.84 GNGITHH95 pKa = 6.78 RR96 pKa = 11.84 VGKK99 pKa = 9.62 RR100 pKa = 11.84 FCIKK104 pKa = 10.12 SVYY107 pKa = 10.1 ILGKK111 pKa = 10.11 VWMDD115 pKa = 4.51 DD116 pKa = 3.58 NIKK119 pKa = 10.64 LKK121 pKa = 10.74 NHH123 pKa = 5.99 TNSVMFWLIRR133 pKa = 11.84 DD134 pKa = 3.59 RR135 pKa = 11.84 RR136 pKa = 11.84 PYY138 pKa = 8.14 GTPMDD143 pKa = 4.6 FGQVFNMYY151 pKa = 10.66 DD152 pKa = 3.86 NEE154 pKa = 4.36 PSTATIKK161 pKa = 10.89 NDD163 pKa = 3.0 LRR165 pKa = 11.84 DD166 pKa = 3.73 RR167 pKa = 11.84 YY168 pKa = 9.95 QVLHH172 pKa = 6.65 RR173 pKa = 11.84 FASKK177 pKa = 9.07 VTGGQYY183 pKa = 11.09 ASNEE187 pKa = 3.76 QSLVRR192 pKa = 11.84 RR193 pKa = 11.84 FWKK196 pKa = 10.44 VNNHH200 pKa = 4.48 VVYY203 pKa = 10.66 NHH205 pKa = 5.91 QEE207 pKa = 3.39 AAKK210 pKa = 10.14 YY211 pKa = 10.5 DD212 pKa = 3.75 NHH214 pKa = 6.5 TEE216 pKa = 4.02 NALLLYY222 pKa = 7.29 MACTHH227 pKa = 7.07 ASNPVYY233 pKa = 9.86 ATLKK237 pKa = 9.47 IRR239 pKa = 11.84 IYY241 pKa = 10.6 FYY243 pKa = 11.36 DD244 pKa = 4.19 SILNN248 pKa = 3.75
Molecular weight: 28.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.531
IPC_protein 9.736
Toseland 10.028
ProMoST 9.794
Dawson 10.262
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.584
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.072
DTASelect 9.97
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.189
Patrickios 9.911
IPC_peptide 10.292
IPC2_peptide 8.873
IPC2.peptide.svr19 8.487
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1552
100
356
221.7
25.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.477 ± 0.775
2.191 ± 0.186
4.961 ± 0.242
4.124 ± 0.724
4.317 ± 0.476
4.961 ± 0.714
3.544 ± 0.421
5.928 ± 0.593
5.67 ± 0.393
7.281 ± 0.601
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.191 ± 0.456
5.863 ± 0.578
5.284 ± 0.527
3.737 ± 0.334
7.539 ± 0.88
9.923 ± 0.92
5.412 ± 0.548
6.186 ± 0.713
1.418 ± 0.281
3.995 ± 0.703
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here