Pseudomonas phage LUZ24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bruynoghevirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9J6Z2|A9J6Z2_BPLUZ Uncharacterized protein gp30 OS=Pseudomonas phage LUZ24 OX=484895 GN=gp30 PE=4 SV=1
MM1 pKa = 7.53ASKK4 pKa = 10.65KK5 pKa = 10.38EE6 pKa = 4.05SLEE9 pKa = 4.0DD10 pKa = 3.66QAWKK14 pKa = 10.46EE15 pKa = 3.53IALEE19 pKa = 4.32KK20 pKa = 10.42EE21 pKa = 4.86FSGSWGGPEE30 pKa = 4.13IDD32 pKa = 5.3ADD34 pKa = 4.14DD35 pKa = 4.73FPLGSACDD43 pKa = 4.21LDD45 pKa = 4.59PEE47 pKa = 4.56VCEE50 pKa = 4.43SCSS53 pKa = 3.26

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9J6T8|A9J6T8_BPLUZ Uncharacterized protein gp2 OS=Pseudomonas phage LUZ24 OX=484895 GN=gp2 PE=4 SV=1
MM1 pKa = 7.24YY2 pKa = 10.33LLEE5 pKa = 4.18GLKK8 pKa = 10.75VKK10 pKa = 10.34GKK12 pKa = 9.04PKK14 pKa = 10.26QRR16 pKa = 11.84TAVYY20 pKa = 9.96KK21 pKa = 10.66GGRR24 pKa = 11.84KK25 pKa = 6.31TAKK28 pKa = 9.78TDD30 pKa = 2.76IHH32 pKa = 6.55RR33 pKa = 11.84NNRR36 pKa = 11.84KK37 pKa = 9.44SVFGSPRR44 pKa = 11.84LGRR47 pKa = 11.84NPLDD51 pKa = 3.26ILINSS56 pKa = 4.13

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13674

27

1055

201.1

22.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.227 ± 0.52

1.134 ± 0.184

6.055 ± 0.218

7.167 ± 0.466

3.817 ± 0.203

8.308 ± 0.433

2.084 ± 0.233

5.119 ± 0.188

5.682 ± 0.275

8.213 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.168

4.351 ± 0.248

4.315 ± 0.22

4.015 ± 0.284

5.872 ± 0.312

5.792 ± 0.286

5.222 ± 0.29

6.984 ± 0.219

1.719 ± 0.133

3.203 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski