Vittaforma corneae (strain ATCC 50505) (Microsporidian parasite) (Nosema corneum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Nosematidae; Vittaforma; Vittaforma corneae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L2GK29|L2GK29_VITCO Uncharacterized protein (Fragment) OS=Vittaforma corneae (strain ATCC 50505) OX=993615 GN=VICG_01727 PE=4 SV=1
MM1 pKa = 7.85PKK3 pKa = 10.01QSTEE7 pKa = 3.59NRR9 pKa = 11.84QDD11 pKa = 3.2SNMIFIDD18 pKa = 4.13DD19 pKa = 4.04PEE21 pKa = 5.19LNEE24 pKa = 4.64EE25 pKa = 4.21YY26 pKa = 10.11PIDD29 pKa = 3.87FTDD32 pKa = 3.68VNSDD36 pKa = 3.34SAGMITIQSSTEE48 pKa = 3.89DD49 pKa = 3.93EE50 pKa = 4.17NTVIVDD56 pKa = 3.67EE57 pKa = 4.93GSLIEE62 pKa = 5.64DD63 pKa = 4.17SLSHH67 pKa = 6.4SNPVFNSSFEE77 pKa = 4.39EE78 pKa = 4.07YY79 pKa = 9.73RR80 pKa = 11.84SSYY83 pKa = 9.54EE84 pKa = 4.01GQVDD88 pKa = 4.31EE89 pKa = 4.85YY90 pKa = 11.59SSFDD94 pKa = 3.44SSGGTLYY101 pKa = 10.96NEE103 pKa = 4.39EE104 pKa = 4.41YY105 pKa = 10.8SFEE108 pKa = 5.04DD109 pKa = 4.75EE110 pKa = 5.61FDD112 pKa = 3.95DD113 pKa = 6.71DD114 pKa = 4.4YY115 pKa = 12.03DD116 pKa = 3.78EE117 pKa = 6.06FEE119 pKa = 5.09DD120 pKa = 4.54EE121 pKa = 4.66EE122 pKa = 4.5EE123 pKa = 3.83EE124 pKa = 4.46SYY126 pKa = 11.48YY127 pKa = 10.95FSDD130 pKa = 5.48DD131 pKa = 4.14LDD133 pKa = 3.82ATLYY137 pKa = 10.86DD138 pKa = 3.73YY139 pKa = 11.61DD140 pKa = 4.48EE141 pKa = 5.26DD142 pKa = 4.27SEE144 pKa = 4.85YY145 pKa = 11.2SDD147 pKa = 3.09EE148 pKa = 4.64VYY150 pKa = 9.85WEE152 pKa = 4.11EE153 pKa = 3.7TSQIVTGMRR162 pKa = 11.84RR163 pKa = 11.84GEE165 pKa = 3.84LSRR168 pKa = 11.84FPEE171 pKa = 4.44NGNSTGADD179 pKa = 3.1RR180 pKa = 11.84NEE182 pKa = 3.92RR183 pKa = 11.84LSAEE187 pKa = 4.27SEE189 pKa = 4.05PDD191 pKa = 3.08EE192 pKa = 5.29HH193 pKa = 7.45EE194 pKa = 4.93LIILEE199 pKa = 4.52SRR201 pKa = 11.84NSDD204 pKa = 3.18QLYY207 pKa = 9.94PRR209 pKa = 11.84VTYY212 pKa = 10.29EE213 pKa = 4.91LITMILVRR221 pKa = 11.84MYY223 pKa = 9.3MAYY226 pKa = 10.63NLGIGEE232 pKa = 4.46YY233 pKa = 9.45YY234 pKa = 10.48SIYY237 pKa = 10.68EE238 pKa = 4.04FDD240 pKa = 3.77SYY242 pKa = 11.35PYY244 pKa = 10.7NDD246 pKa = 3.44VNIADD251 pKa = 5.17KK252 pKa = 10.94IRR254 pKa = 11.84DD255 pKa = 3.86FTPDD259 pKa = 3.01EE260 pKa = 4.17LSFLKK265 pKa = 10.81SRR267 pKa = 11.84VSSILMNDD275 pKa = 2.67IRR277 pKa = 11.84DD278 pKa = 3.81VSSVQALISLISIDD292 pKa = 5.23DD293 pKa = 3.31IDD295 pKa = 4.86NISFSSFKK303 pKa = 9.34KK304 pKa = 9.11TKK306 pKa = 10.05FKK308 pKa = 10.73STMFKK313 pKa = 11.35DD314 pKa = 3.94MINVDD319 pKa = 3.79GKK321 pKa = 11.2SCGICYY327 pKa = 10.15CDD329 pKa = 4.02FRR331 pKa = 11.84PSHH334 pKa = 6.62KK335 pKa = 9.66CTSLQCLHH343 pKa = 6.41TFHH346 pKa = 6.76TKK348 pKa = 10.5CIKK351 pKa = 10.28KK352 pKa = 8.57WVAVSWCCPLCRR364 pKa = 11.84RR365 pKa = 11.84KK366 pKa = 10.67DD367 pKa = 3.3IGG369 pKa = 3.52

Molecular weight: 42.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L2GQ25|L2GQ25_VITCO Glutamyl-tRNA synthetase OS=Vittaforma corneae (strain ATCC 50505) OX=993615 GN=VICG_00261 PE=3 SV=1
MM1 pKa = 7.75AGRR4 pKa = 11.84GKK6 pKa = 10.15GAKK9 pKa = 8.61GAKK12 pKa = 9.2GVAKK16 pKa = 10.26RR17 pKa = 11.84HH18 pKa = 5.75RR19 pKa = 11.84KK20 pKa = 8.74QPSSSSKK27 pKa = 10.05ISGPAIRR34 pKa = 11.84RR35 pKa = 11.84LARR38 pKa = 11.84RR39 pKa = 11.84AGVPRR44 pKa = 11.84VGAGCFPEE52 pKa = 4.66VNDD55 pKa = 4.25AAVNYY60 pKa = 7.52LTTLLDD66 pKa = 3.32SCRR69 pKa = 11.84IYY71 pKa = 10.76CSHH74 pKa = 7.21SKK76 pKa = 10.5RR77 pKa = 11.84KK78 pKa = 8.07TINCQDD84 pKa = 3.25VIYY87 pKa = 10.45ALKK90 pKa = 10.67RR91 pKa = 11.84LGTKK95 pKa = 10.53YY96 pKa = 10.04MGFF99 pKa = 3.7

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2236

0

2236

714266

39

2539

319.4

36.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.547 ± 0.036

2.036 ± 0.024

5.507 ± 0.037

7.179 ± 0.05

5.647 ± 0.041

4.341 ± 0.038

1.913 ± 0.019

8.174 ± 0.043

8.357 ± 0.049

9.972 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.018

5.913 ± 0.042

3.142 ± 0.034

3.033 ± 0.024

4.407 ± 0.037

8.351 ± 0.051

4.803 ± 0.024

5.854 ± 0.04

0.574 ± 0.011

3.933 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski