Mycobacterium phage Odin
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5M9W5|S5M9W5_9CAUD Uncharacterized protein OS=Mycobacterium phage Odin OX=1340711 GN=85 PE=4 SV=1
MM1 pKa = 7.76 IEE3 pKa = 4.01 TLGDD7 pKa = 3.4 IDD9 pKa = 4.65 QMARR13 pKa = 11.84 NPKK16 pKa = 8.8 TGRR19 pKa = 11.84 EE20 pKa = 4.12 PGDD23 pKa = 3.18 NTYY26 pKa = 10.92 NCTWAAVALSAYY38 pKa = 7.91 TKK40 pKa = 10.87 VVGGDD45 pKa = 3.57 DD46 pKa = 4.11 NEE48 pKa = 4.47 VGTQISDD55 pKa = 3.68 LLGDD59 pKa = 4.65 LLHH62 pKa = 6.89 LADD65 pKa = 3.94 AAGVDD70 pKa = 4.61 FYY72 pKa = 11.56 EE73 pKa = 4.65 ALATAEE79 pKa = 4.27 TNYY82 pKa = 10.12 KK83 pKa = 10.72 AEE85 pKa = 4.43 VNGEE89 pKa = 3.97
Molecular weight: 9.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.018
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.668
ProMoST 3.986
Dawson 3.859
Bjellqvist 4.088
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 2.994
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|S5M3Q5|S5M3Q5_9CAUD Uncharacterized protein OS=Mycobacterium phage Odin OX=1340711 GN=41 PE=4 SV=1
MM1 pKa = 7.9 PPRR4 pKa = 11.84 ASIQEE9 pKa = 4.04 TADD12 pKa = 3.51 YY13 pKa = 10.85 LGVSTKK19 pKa = 7.22 TVRR22 pKa = 11.84 NYY24 pKa = 10.21 IADD27 pKa = 3.64 GRR29 pKa = 11.84 LKK31 pKa = 10.45 AVRR34 pKa = 11.84 LGPRR38 pKa = 11.84 LIRR41 pKa = 11.84 VEE43 pKa = 4.12 RR44 pKa = 11.84 EE45 pKa = 3.81 SVEE48 pKa = 3.94 ALMRR52 pKa = 11.84 PIGKK56 pKa = 9.51
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.677
IPC_protein 10.584
Toseland 10.643
ProMoST 10.511
Dawson 10.76
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.613
DTASelect 10.482
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.311
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
94
0
94
15944
38
841
169.6
18.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.765 ± 0.392
0.872 ± 0.12
6.228 ± 0.21
6.767 ± 0.3
3.249 ± 0.163
8.034 ± 0.344
2.07 ± 0.172
5.206 ± 0.192
4.93 ± 0.254
7.972 ± 0.345
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.572 ± 0.137
3.412 ± 0.199
5.381 ± 0.252
3.788 ± 0.173
6.284 ± 0.343
5.526 ± 0.201
6.052 ± 0.231
7.094 ± 0.171
1.907 ± 0.135
2.891 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here