Ruminococcus sp. CAG:563

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6ELE6|R6ELE6_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:563 OX=1262961 GN=BN710_00994 PE=4 SV=1
MM1 pKa = 7.92ALFTNQATLTYY12 pKa = 9.21NDD14 pKa = 3.61NVINSNIVTGEE25 pKa = 3.7IVQVLTVTKK34 pKa = 10.4NALSDD39 pKa = 3.22TYY41 pKa = 11.2EE42 pKa = 3.98RR43 pKa = 11.84DD44 pKa = 3.01KK45 pKa = 11.2TITYY49 pKa = 9.83IINIVNSSDD58 pKa = 4.08SDD60 pKa = 3.89FTDD63 pKa = 3.57LTVTDD68 pKa = 4.08NLGAYY73 pKa = 9.16AFNAAALVPLTYY85 pKa = 10.98VNDD88 pKa = 3.58SVRR91 pKa = 11.84YY92 pKa = 8.61FIKK95 pKa = 10.62GDD97 pKa = 3.66LQSAPTVAAVSPLTITGITVPANGNATVVYY127 pKa = 9.71SATVNEE133 pKa = 4.45FAPLDD138 pKa = 3.85GEE140 pKa = 4.27AQITNTVTVSGIGLSSPVTAEE161 pKa = 3.63EE162 pKa = 4.62TITLEE167 pKa = 3.94TGPILSITKK176 pKa = 9.58AVCPLSVPEE185 pKa = 4.94NGTLTYY191 pKa = 10.66TFVIRR196 pKa = 11.84NNGFTEE202 pKa = 3.99ATAADD207 pKa = 3.81NLVIRR212 pKa = 11.84DD213 pKa = 3.9TFDD216 pKa = 5.16PILNITSVTLDD227 pKa = 3.47GTALTPTTGYY237 pKa = 9.24TYY239 pKa = 10.71NAEE242 pKa = 4.16TGEE245 pKa = 4.27FATVAGAVTVPAATYY260 pKa = 10.73VRR262 pKa = 11.84DD263 pKa = 3.9ATTGAYY269 pKa = 10.22SITPGATVLTVTGTVV284 pKa = 3.08

Molecular weight: 29.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6E4F9|R6E4F9_9FIRM Oxidoreductase OS=Ruminococcus sp. CAG:563 OX=1262961 GN=BN710_01522 PE=4 SV=1
MM1 pKa = 7.06YY2 pKa = 10.53GRR4 pKa = 11.84YY5 pKa = 9.96GLDD8 pKa = 2.93KK9 pKa = 10.62LTGFLILLNLLLNGIGSFMRR29 pKa = 11.84NRR31 pKa = 11.84IAYY34 pKa = 8.53YY35 pKa = 9.57IFYY38 pKa = 10.41VLALAVFGFAVFRR51 pKa = 11.84VLSKK55 pKa = 10.88NIEE58 pKa = 3.68KK59 pKa = 10.38RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84EE63 pKa = 4.21GEE65 pKa = 3.74WFDD68 pKa = 3.61NLLVRR73 pKa = 11.84TNYY76 pKa = 9.37TEE78 pKa = 4.37HH79 pKa = 5.66FRR81 pKa = 11.84KK82 pKa = 8.61MKK84 pKa = 10.16RR85 pKa = 11.84WFKK88 pKa = 10.49RR89 pKa = 11.84QGLRR93 pKa = 11.84FKK95 pKa = 10.79YY96 pKa = 10.18FKK98 pKa = 8.04THH100 pKa = 6.65RR101 pKa = 11.84FRR103 pKa = 11.84VCPNCGEE110 pKa = 4.05NLRR113 pKa = 11.84LSKK116 pKa = 10.73KK117 pKa = 9.45RR118 pKa = 11.84GKK120 pKa = 10.62RR121 pKa = 11.84NITCPICGNKK131 pKa = 8.0FTIHH135 pKa = 6.52ILFF138 pKa = 4.36

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2054

0

2054

666546

29

2645

324.5

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.526 ± 0.064

1.705 ± 0.026

6.262 ± 0.053

6.749 ± 0.056

4.481 ± 0.048

7.03 ± 0.052

1.566 ± 0.028

7.732 ± 0.049

7.586 ± 0.046

8.121 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.031

4.937 ± 0.043

3.247 ± 0.028

2.326 ± 0.023

3.87 ± 0.041

6.419 ± 0.06

5.671 ± 0.053

7.023 ± 0.043

0.847 ± 0.02

4.158 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski