Amino acid dipepetide frequency for Ruminococcus sp. CAG:563

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.024AlaAla: 7.024 ± 0.142	1.152AlaCys: 1.152 ± 0.052	5.062AlaAsp: 5.062 ± 0.095	5.571AlaGlu: 5.571 ± 0.104	3.35AlaPhe: 3.35 ± 0.079	4.919AlaGly: 4.919 ± 0.118	1.115AlaHis: 1.115 ± 0.037	5.371AlaIle: 5.371 ± 0.099	5.602AlaLys: 5.602 ± 0.093	6.448AlaLeu: 6.448 ± 0.117	2.246AlaMet: 2.246 ± 0.063	2.98AlaAsn: 2.98 ± 0.077	2.124AlaPro: 2.124 ± 0.057	2.129AlaGln: 2.129 ± 0.061	2.553AlaArg: 2.553 ± 0.068	4.12AlaSer: 4.12 ± 0.086	3.746AlaThr: 3.746 ± 0.098	6.529AlaVal: 6.529 ± 0.126	0.561AlaTrp: 0.561 ± 0.031	2.66AlaTyr: 2.66 ± 0.064	0.002AlaXaa: 0.002 ± 0.001
Cys	1.241CysAla: 1.241 ± 0.046	0.357CysCys: 0.357 ± 0.025	1.241CysAsp: 1.241 ± 0.045	1.193CysGlu: 1.193 ± 0.045	0.738CysPhe: 0.738 ± 0.035	1.736CysGly: 1.736 ± 0.06	0.305CysHis: 0.305 ± 0.022	1.199CysIle: 1.199 ± 0.044	1.154CysLys: 1.154 ± 0.045	1.097CysLeu: 1.097 ± 0.042	0.401CysMet: 0.401 ± 0.021	0.701CysAsn: 0.701 ± 0.033	0.698CysPro: 0.698 ± 0.04	0.311CysGln: 0.311 ± 0.021	0.725CysArg: 0.725 ± 0.03	1.14CysSer: 1.14 ± 0.044	0.897CysThr: 0.897 ± 0.047	1.211CysVal: 1.211 ± 0.047	0.125CysTrp: 0.125 ± 0.015	0.581CysTyr: 0.581 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.328AspAla: 4.328 ± 0.085	1.049AspCys: 1.049 ± 0.043	3.772AspAsp: 3.772 ± 0.094	5.272AspGlu: 5.272 ± 0.106	3.331AspPhe: 3.331 ± 0.072	5.548AspGly: 5.548 ± 0.106	0.81AspHis: 0.81 ± 0.04	5.153AspIle: 5.153 ± 0.087	4.669AspLys: 4.669 ± 0.088	4.327AspLeu: 4.327 ± 0.084	1.746AspMet: 1.746 ± 0.059	2.872AspAsn: 2.872 ± 0.075	1.82AspPro: 1.82 ± 0.062	0.881AspGln: 0.881 ± 0.038	2.427AspArg: 2.427 ± 0.066	3.809AspSer: 3.809 ± 0.089	3.058AspThr: 3.058 ± 0.064	4.19AspVal: 4.19 ± 0.089	0.584AspTrp: 0.584 ± 0.028	2.971AspTyr: 2.971 ± 0.074	0.002AspXaa: 0.002 ± 0.002
Glu	4.568GluAla: 4.568 ± 0.094	0.957GluCys: 0.957 ± 0.034	3.461GluAsp: 3.461 ± 0.08	4.64GluGlu: 4.64 ± 0.092	2.667GluPhe: 2.667 ± 0.069	3.46GluGly: 3.46 ± 0.078	1.133GluHis: 1.133 ± 0.048	5.814GluIle: 5.814 ± 0.09	6.913GluLys: 6.913 ± 0.12	6.154GluLeu: 6.154 ± 0.118	2.061GluMet: 2.061 ± 0.051	4.51GluAsn: 4.51 ± 0.083	1.799GluPro: 1.799 ± 0.053	1.877GluGln: 1.877 ± 0.056	2.906GluArg: 2.906 ± 0.07	3.592GluSer: 3.592 ± 0.075	3.49GluThr: 3.49 ± 0.078	3.878GluVal: 3.878 ± 0.076	0.641GluTrp: 0.641 ± 0.029	2.968GluTyr: 2.968 ± 0.084	0.0GluXaa: 0.0 ± 0.0
Phe	3.46PheAla: 3.46 ± 0.079	0.867PheCys: 0.867 ± 0.038	3.364PheAsp: 3.364 ± 0.075	2.743PheGlu: 2.743 ± 0.064	2.129PhePhe: 2.129 ± 0.069	3.518PheGly: 3.518 ± 0.077	0.641PheHis: 0.641 ± 0.033	3.287PheIle: 3.287 ± 0.073	2.879PheLys: 2.879 ± 0.067	3.44PheLeu: 3.44 ± 0.09	1.179PheMet: 1.179 ± 0.045	2.178PheAsn: 2.178 ± 0.049	1.461PhePro: 1.461 ± 0.05	0.767PheGln: 0.767 ± 0.033	1.568PheArg: 1.568 ± 0.05	3.412PheSer: 3.412 ± 0.074	2.699PheThr: 2.699 ± 0.064	3.1PheVal: 3.1 ± 0.077	0.383PheTrp: 0.383 ± 0.024	1.737PheTyr: 1.737 ± 0.06	0.0PheXaa: 0.0 ± 0.0
Gly	4.928GlyAla: 4.928 ± 0.108	1.365GlyCys: 1.365 ± 0.049	3.98GlyAsp: 3.98 ± 0.076	4.726GlyGlu: 4.726 ± 0.088	3.397GlyPhe: 3.397 ± 0.085	4.817GlyGly: 4.817 ± 0.124	1.233GlyHis: 1.233 ± 0.047	5.953GlyIle: 5.953 ± 0.115	5.856GlyLys: 5.856 ± 0.089	5.257GlyLeu: 5.257 ± 0.099	2.157GlyMet: 2.157 ± 0.062	3.272GlyAsn: 3.272 ± 0.075	1.071GlyPro: 1.071 ± 0.043	1.568GlyGln: 1.568 ± 0.047	2.833GlyArg: 2.833 ± 0.075	4.358GlySer: 4.358 ± 0.099	4.402GlyThr: 4.402 ± 0.099	5.048GlyVal: 5.048 ± 0.098	0.699GlyTrp: 0.699 ± 0.036	3.374GlyTyr: 3.374 ± 0.087	0.0GlyXaa: 0.0 ± 0.0
His	0.932HisAla: 0.932 ± 0.039	0.339HisCys: 0.339 ± 0.025	1.013HisAsp: 1.013 ± 0.039	0.995HisGlu: 0.995 ± 0.038	0.716HisPhe: 0.716 ± 0.034	1.245HisGly: 1.245 ± 0.042	0.362HisHis: 0.362 ± 0.022	1.251HisIle: 1.251 ± 0.051	0.987HisLys: 0.987 ± 0.037	1.142HisLeu: 1.142 ± 0.047	0.344HisMet: 0.344 ± 0.023	0.869HisAsn: 0.869 ± 0.04	0.764HisPro: 0.764 ± 0.036	0.333HisGln: 0.333 ± 0.023	0.72HisArg: 0.72 ± 0.035	1.031HisSer: 1.031 ± 0.044	0.855HisThr: 0.855 ± 0.038	0.906HisVal: 0.906 ± 0.035	0.174HisTrp: 0.174 ± 0.018	0.687HisTyr: 0.687 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.211IleAla: 6.211 ± 0.11	1.445IleCys: 1.445 ± 0.045	4.936IleAsp: 4.936 ± 0.083	4.985IleGlu: 4.985 ± 0.1	3.248IlePhe: 3.248 ± 0.071	5.374IleGly: 5.374 ± 0.102	1.115IleHis: 1.115 ± 0.042	6.109IleIle: 6.109 ± 0.114	5.776IleLys: 5.776 ± 0.084	6.088IleLeu: 6.088 ± 0.11	1.947IleMet: 1.947 ± 0.053	3.805IleAsn: 3.805 ± 0.074	3.056IlePro: 3.056 ± 0.069	1.463IleGln: 1.463 ± 0.05	2.909IleArg: 2.909 ± 0.073	5.474IleSer: 5.474 ± 0.11	4.556IleThr: 4.556 ± 0.089	5.473IleVal: 5.473 ± 0.096	0.522IleTrp: 0.522 ± 0.026	2.83IleTyr: 2.83 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	5.97LysAla: 5.97 ± 0.101	0.984LysCys: 0.984 ± 0.044	4.294LysAsp: 4.294 ± 0.093	5.258LysGlu: 5.258 ± 0.108	2.801LysPhe: 2.801 ± 0.068	4.282LysGly: 4.282 ± 0.082	1.032LysHis: 1.032 ± 0.041	6.279LysIle: 6.279 ± 0.103	7.077LysLys: 7.077 ± 0.126	6.288LysLeu: 6.288 ± 0.099	2.334LysMet: 2.334 ± 0.056	4.511LysAsn: 4.511 ± 0.087	2.346LysPro: 2.346 ± 0.064	1.917LysGln: 1.917 ± 0.062	3.055LysArg: 3.055 ± 0.071	4.312LysSer: 4.312 ± 0.074	4.43LysThr: 4.43 ± 0.075	4.544LysVal: 4.544 ± 0.082	0.59LysTrp: 0.59 ± 0.034	3.557LysTyr: 3.557 ± 0.092	0.0LysXaa: 0.0 ± 0.0
Leu	5.971LeuAla: 5.971 ± 0.087	1.76LeuCys: 1.76 ± 0.052	5.188LeuAsp: 5.188 ± 0.091	4.846LeuGlu: 4.846 ± 0.096	3.617LeuPhe: 3.617 ± 0.096	5.598LeuGly: 5.598 ± 0.089	1.263LeuHis: 1.263 ± 0.038	6.006LeuIle: 6.006 ± 0.124	5.974LeuLys: 5.974 ± 0.102	6.799LeuLeu: 6.799 ± 0.133	2.22LeuMet: 2.22 ± 0.064	3.995LeuAsn: 3.995 ± 0.087	3.266LeuPro: 3.266 ± 0.07	1.851LeuGln: 1.851 ± 0.049	3.286LeuArg: 3.286 ± 0.074	6.022LeuSer: 6.022 ± 0.102	4.843LeuThr: 4.843 ± 0.095	5.089LeuVal: 5.089 ± 0.092	0.624LeuTrp: 0.624 ± 0.03	2.989LeuTyr: 2.989 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	2.057MetAla: 2.057 ± 0.069	0.392MetCys: 0.392 ± 0.028	1.449MetAsp: 1.449 ± 0.051	1.467MetGlu: 1.467 ± 0.052	1.098MetPhe: 1.098 ± 0.042	1.847MetGly: 1.847 ± 0.057	0.42MetHis: 0.42 ± 0.029	1.956MetIle: 1.956 ± 0.054	2.565MetLys: 2.565 ± 0.062	2.648MetLeu: 2.648 ± 0.069	0.795MetMet: 0.795 ± 0.036	1.533MetAsn: 1.533 ± 0.046	1.176MetPro: 1.176 ± 0.044	0.783MetGln: 0.783 ± 0.036	1.121MetArg: 1.121 ± 0.04	1.853MetSer: 1.853 ± 0.056	1.715MetThr: 1.715 ± 0.047	1.452MetVal: 1.452 ± 0.048	0.203MetTrp: 0.203 ± 0.019	0.894MetTyr: 0.894 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	3.694AsnAla: 3.694 ± 0.081	0.858AsnCys: 0.858 ± 0.035	3.053AsnAsp: 3.053 ± 0.068	3.259AsnGlu: 3.259 ± 0.072	2.052AsnPhe: 2.052 ± 0.055	4.576AsnGly: 4.576 ± 0.113	0.777AsnHis: 0.777 ± 0.037	3.862AsnIle: 3.862 ± 0.072	3.389AsnLys: 3.389 ± 0.074	3.602AsnLeu: 3.602 ± 0.079	1.266AsnMet: 1.266 ± 0.043	2.351AsnAsn: 2.351 ± 0.067	1.982AsnPro: 1.982 ± 0.055	1.028AsnGln: 1.028 ± 0.045	1.812AsnArg: 1.812 ± 0.054	3.242AsnSer: 3.242 ± 0.081	2.529AsnThr: 2.529 ± 0.068	3.515AsnVal: 3.515 ± 0.08	0.399AsnTrp: 0.399 ± 0.024	2.124AsnTyr: 2.124 ± 0.061	0.0AsnXaa: 0.0 ± 0.0
Pro	2.127ProAla: 2.127 ± 0.05	0.521ProCys: 0.521 ± 0.029	2.453ProAsp: 2.453 ± 0.071	3.016ProGlu: 3.016 ± 0.059	1.551ProPhe: 1.551 ± 0.049	1.868ProGly: 1.868 ± 0.057	0.581ProHis: 0.581 ± 0.032	2.445ProIle: 2.445 ± 0.069	2.142ProLys: 2.142 ± 0.055	2.543ProLeu: 2.543 ± 0.069	0.834ProMet: 0.834 ± 0.038	1.482ProAsn: 1.482 ± 0.044	0.708ProPro: 0.708 ± 0.035	0.972ProGln: 0.972 ± 0.038	0.936ProArg: 0.936 ± 0.04	2.042ProSer: 2.042 ± 0.057	1.968ProThr: 1.968 ± 0.067	2.538ProVal: 2.538 ± 0.055	0.308ProTrp: 0.308 ± 0.024	1.434ProTyr: 1.434 ± 0.053	0.0ProXaa: 0.0 ± 0.0
Gln	1.821GlnAla: 1.821 ± 0.058	0.351GlnCys: 0.351 ± 0.022	1.025GlnAsp: 1.025 ± 0.046	1.25GlnGlu: 1.25 ± 0.045	0.912GlnPhe: 0.912 ± 0.039	1.403GlnGly: 1.403 ± 0.046	0.369GlnHis: 0.369 ± 0.024	1.884GlnIle: 1.884 ± 0.055	2.034GlnLys: 2.034 ± 0.053	2.066GlnLeu: 2.066 ± 0.065	0.684GlnMet: 0.684 ± 0.032	1.305GlnAsn: 1.305 ± 0.047	0.765GlnPro: 0.765 ± 0.034	0.689GlnGln: 0.689 ± 0.041	1.107GlnArg: 1.107 ± 0.041	1.551GlnSer: 1.551 ± 0.058	1.407GlnThr: 1.407 ± 0.048	1.421GlnVal: 1.421 ± 0.048	0.234GlnTrp: 0.234 ± 0.02	0.984GlnTyr: 0.984 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.534ArgAla: 2.534 ± 0.063	0.588ArgCys: 0.588 ± 0.031	2.166ArgAsp: 2.166 ± 0.063	2.873ArgGlu: 2.873 ± 0.076	1.916ArgPhe: 1.916 ± 0.057	2.129ArgGly: 2.129 ± 0.065	0.689ArgHis: 0.689 ± 0.027	3.367ArgIle: 3.367 ± 0.081	2.864ArgLys: 2.864 ± 0.073	3.431ArgLeu: 3.431 ± 0.076	1.131ArgMet: 1.131 ± 0.04	1.946ArgAsn: 1.946 ± 0.053	1.203ArgPro: 1.203 ± 0.047	1.067ArgGln: 1.067 ± 0.04	1.862ArgArg: 1.862 ± 0.06	2.253ArgSer: 2.253 ± 0.061	2.138ArgThr: 2.138 ± 0.062	2.472ArgVal: 2.472 ± 0.065	0.326ArgTrp: 0.326 ± 0.022	1.745ArgTyr: 1.745 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	4.97SerAla: 4.97 ± 0.089	0.963SerCys: 0.963 ± 0.044	4.312SerAsp: 4.312 ± 0.087	4.252SerGlu: 4.252 ± 0.077	3.271SerPhe: 3.271 ± 0.07	5.272SerGly: 5.272 ± 0.107	1.047SerHis: 1.047 ± 0.043	4.343SerIle: 4.343 ± 0.083	4.166SerLys: 4.166 ± 0.077	5.321SerLeu: 5.321 ± 0.095	1.601SerMet: 1.601 ± 0.055	2.685SerAsn: 2.685 ± 0.078	1.868SerPro: 1.868 ± 0.051	1.604SerGln: 1.604 ± 0.056	2.316SerArg: 2.316 ± 0.069	4.336SerSer: 4.336 ± 0.121	3.299SerThr: 3.299 ± 0.087	5.396SerVal: 5.396 ± 0.117	0.581SerTrp: 0.581 ± 0.032	2.588SerTyr: 2.588 ± 0.078	0.0SerXaa: 0.0 ± 0.0
Thr	5.075ThrAla: 5.075 ± 0.104	0.816ThrCys: 0.816 ± 0.05	3.916ThrAsp: 3.916 ± 0.092	3.64ThrGlu: 3.64 ± 0.073	2.327ThrPhe: 2.327 ± 0.067	4.436ThrGly: 4.436 ± 0.091	0.917ThrHis: 0.917 ± 0.039	3.802ThrIle: 3.802 ± 0.089	3.302ThrLys: 3.302 ± 0.074	4.829ThrLeu: 4.829 ± 0.081	1.307ThrMet: 1.307 ± 0.046	2.181ThrAsn: 2.181 ± 0.061	2.216ThrPro: 2.216 ± 0.058	1.323ThrGln: 1.323 ± 0.045	1.85ThrArg: 1.85 ± 0.06	3.166ThrSer: 3.166 ± 0.09	3.191ThrThr: 3.191 ± 0.09	5.659ThrVal: 5.659 ± 0.13	0.474ThrTrp: 0.474 ± 0.028	2.285ThrTyr: 2.285 ± 0.07	0.0ThrXaa: 0.0 ± 0.0
Val	5.041ValAla: 5.041 ± 0.119	1.398ValCys: 1.398 ± 0.052	4.372ValAsp: 4.372 ± 0.09	4.472ValGlu: 4.472 ± 0.084	3.19ValPhe: 3.19 ± 0.073	4.565ValGly: 4.565 ± 0.085	0.977ValHis: 0.977 ± 0.041	5.586ValIle: 5.586 ± 0.091	5.083ValLys: 5.083 ± 0.092	5.983ValLeu: 5.983 ± 0.094	1.968ValMet: 1.968 ± 0.057	3.358ValAsn: 3.358 ± 0.074	2.58ValPro: 2.58 ± 0.061	1.5ValGln: 1.5 ± 0.048	2.753ValArg: 2.753 ± 0.065	4.939ValSer: 4.939 ± 0.104	4.42ValThr: 4.42 ± 0.128	4.754ValVal: 4.754 ± 0.092	0.578ValTrp: 0.578 ± 0.03	2.712ValTyr: 2.712 ± 0.068	0.0ValXaa: 0.0 ± 0.0
Trp	0.579TrpAla: 0.579 ± 0.029	0.146TrpCys: 0.146 ± 0.015	0.554TrpAsp: 0.554 ± 0.027	0.503TrpGlu: 0.503 ± 0.03	0.45TrpPhe: 0.45 ± 0.025	0.602TrpGly: 0.602 ± 0.033	0.2TrpHis: 0.2 ± 0.019	0.581TrpIle: 0.581 ± 0.033	0.558TrpLys: 0.558 ± 0.031	0.786TrpLeu: 0.786 ± 0.039	0.239TrpMet: 0.239 ± 0.018	0.498TrpAsn: 0.498 ± 0.027	0.159TrpPro: 0.159 ± 0.015	0.335TrpGln: 0.335 ± 0.024	0.302TrpArg: 0.302 ± 0.023	0.515TrpSer: 0.515 ± 0.033	0.441TrpThr: 0.441 ± 0.029	0.486TrpVal: 0.486 ± 0.028	0.11TrpTrp: 0.11 ± 0.013	0.432TrpTyr: 0.432 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.732TyrAla: 2.732 ± 0.071	0.738TyrCys: 0.738 ± 0.033	3.008TyrAsp: 3.008 ± 0.075	2.529TyrGlu: 2.529 ± 0.063	2.049TyrPhe: 2.049 ± 0.057	3.1TyrGly: 3.1 ± 0.069	0.68TyrHis: 0.68 ± 0.034	3.076TyrIle: 3.076 ± 0.066	2.87TyrLys: 2.87 ± 0.07	2.957TyrLeu: 2.957 ± 0.071	0.96TyrMet: 0.96 ± 0.039	2.339TyrAsn: 2.339 ± 0.061	1.406TyrPro: 1.406 ± 0.051	0.867TyrGln: 0.867 ± 0.037	1.659TyrArg: 1.659 ± 0.053	3.028TyrSer: 3.028 ± 0.08	2.627TyrThr: 2.627 ± 0.087	2.565TyrVal: 2.565 ± 0.063	0.36TyrTrp: 0.36 ± 0.024	2.031TyrTyr: 2.031 ± 0.08	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.002XaaMet: 0.002 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 2054 proteins (666546 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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