Bifidobacterium mongoliense DSM 21395
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1798 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A087C160|A0A087C160_9BIFI Polyprenyl synthetase OS=Bifidobacterium mongoliense DSM 21395 OX=1437603 GN=BMON_0566 PE=3 SV=1
MM1 pKa = 7.13 AQDD4 pKa = 3.51 YY5 pKa = 10.08 DD6 pKa = 3.82 SPRR9 pKa = 11.84 NKK11 pKa = 10.87 DD12 pKa = 3.25 EE13 pKa = 5.64 DD14 pKa = 4.36 DD15 pKa = 4.47 EE16 pKa = 4.64 SLQALGKK23 pKa = 10.46 GSQNTGGEE31 pKa = 4.16 DD32 pKa = 3.6 DD33 pKa = 5.2 DD34 pKa = 4.91 EE35 pKa = 4.35 NAIAEE40 pKa = 4.35 DD41 pKa = 4.23 YY42 pKa = 9.11 EE43 pKa = 5.05 LPGADD48 pKa = 5.03 LSNEE52 pKa = 4.1 DD53 pKa = 3.77 ASVTVIPMQGDD64 pKa = 3.48 EE65 pKa = 5.44 FICSEE70 pKa = 4.23 CFLVKK75 pKa = 10.28 HH76 pKa = 6.48 RR77 pKa = 11.84 SQLAYY82 pKa = 7.08 TTADD86 pKa = 3.42 GQPVCKK92 pKa = 10.15 EE93 pKa = 3.94 CAAA96 pKa = 4.21
Molecular weight: 10.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A087C1L1|A0A087C1L1_9BIFI Chromosome partitioning protein OS=Bifidobacterium mongoliense DSM 21395 OX=1437603 GN=BMON_1257 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.83 MKK15 pKa = 9.27 HH16 pKa = 5.56 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 GKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.92 SLSAA44 pKa = 3.86
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1798
0
1798
621834
31
1779
345.8
37.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.143 ± 0.07
0.86 ± 0.019
6.637 ± 0.052
5.089 ± 0.063
3.231 ± 0.038
8.432 ± 0.054
2.524 ± 0.031
5.112 ± 0.042
3.131 ± 0.057
8.877 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.681 ± 0.025
2.864 ± 0.036
4.804 ± 0.037
3.419 ± 0.033
6.851 ± 0.068
6.383 ± 0.049
6.177 ± 0.048
7.895 ± 0.048
1.363 ± 0.024
2.524 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here