Faeces associated gemycircularvirus 5
Average proteome isoelectric point is 7.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1YRW5|T1YRW5_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 5 OX=1391034 PE=4 SV=1
MM1 pKa = 7.34 TFILNARR8 pKa = 11.84 YY9 pKa = 8.98 FLVTYY14 pKa = 7.78 PQSDD18 pKa = 4.04 GLDD21 pKa = 2.95 EE22 pKa = 4.22 WAVNDD27 pKa = 3.93 HH28 pKa = 6.47 FGSLGAEE35 pKa = 3.91 CIVARR40 pKa = 11.84 EE41 pKa = 3.93 NHH43 pKa = 5.82 AVRR46 pKa = 11.84 GTHH49 pKa = 6.25 LHH51 pKa = 5.32 VFCDD55 pKa = 4.88 FGRR58 pKa = 11.84 KK59 pKa = 8.2 FRR61 pKa = 11.84 SRR63 pKa = 11.84 RR64 pKa = 11.84 ADD66 pKa = 3.04 IFDD69 pKa = 3.3 VGGFHH74 pKa = 7.62 PNIEE78 pKa = 3.93 RR79 pKa = 11.84 SKK81 pKa = 9.8 RR82 pKa = 11.84 NPRR85 pKa = 11.84 KK86 pKa = 9.72 GALYY90 pKa = 10.12 ACKK93 pKa = 10.51 DD94 pKa = 3.31 GDD96 pKa = 3.9 IVAGGLDD103 pKa = 3.83 VPGLATSIVSAAQDD117 pKa = 3.39 PGATLVCAEE126 pKa = 4.19 SQRR129 pKa = 11.84 EE130 pKa = 4.04 FFEE133 pKa = 4.36 LAEE136 pKa = 5.66 DD137 pKa = 3.75 ICPWDD142 pKa = 5.07 LITKK146 pKa = 8.77 FGSMHH151 pKa = 7.37 AYY153 pKa = 10.05 AKK155 pKa = 9.95 WKK157 pKa = 10.06 FPEE160 pKa = 4.06 LAEE163 pKa = 4.64 PYY165 pKa = 9.08 EE166 pKa = 4.38 NPAGFTLADD175 pKa = 3.64 GAFPDD180 pKa = 3.96 LVSWRR185 pKa = 11.84 TGALEE190 pKa = 3.52 HH191 pKa = 6.61 SGRR194 pKa = 11.84 IKK196 pKa = 10.88 SLVLIGDD203 pKa = 4.37 ALTGKK208 pKa = 6.14 TTWARR213 pKa = 11.84 MLGNHH218 pKa = 7.21 LYY220 pKa = 9.94 MKK222 pKa = 10.07 EE223 pKa = 3.82 RR224 pKa = 11.84 YY225 pKa = 8.1 NAKK228 pKa = 9.6 QASLADD234 pKa = 3.69 GVDD237 pKa = 3.29 YY238 pKa = 11.28 GVIDD242 pKa = 5.66 DD243 pKa = 3.81 ISGGIKK249 pKa = 10.11 YY250 pKa = 9.94 FPHH253 pKa = 5.57 WKK255 pKa = 9.37 SWFGGQPHH263 pKa = 6.38 IQVKK267 pKa = 9.99 ILYY270 pKa = 9.57 KK271 pKa = 10.14 DD272 pKa = 3.3 EE273 pKa = 5.6 RR274 pKa = 11.84 LVKK277 pKa = 9.28 WGKK280 pKa = 8.58 PLIWIANRR288 pKa = 11.84 DD289 pKa = 3.54 PRR291 pKa = 11.84 DD292 pKa = 3.29 QLRR295 pKa = 11.84 DD296 pKa = 3.62 MVSRR300 pKa = 11.84 DD301 pKa = 3.53 YY302 pKa = 11.67 SEE304 pKa = 4.38 EE305 pKa = 3.73 QCNNDD310 pKa = 3.83 VYY312 pKa = 10.91 WMEE315 pKa = 4.11 GNAIFVDD322 pKa = 3.97 IRR324 pKa = 11.84 QSSIISHH331 pKa = 6.89 ANTEE335 pKa = 4.12
Molecular weight: 37.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.741
IPC2_protein 5.677
IPC_protein 5.728
Toseland 6.148
ProMoST 5.97
Dawson 6.008
Bjellqvist 6.008
Wikipedia 6.02
Rodwell 5.982
Grimsley 6.3
Solomon 6.008
Lehninger 5.995
Nozaki 6.275
DTASelect 6.465
Thurlkill 6.465
EMBOSS 6.44
Sillero 6.376
Patrickios 4.139
IPC_peptide 6.02
IPC2_peptide 6.364
IPC2.peptide.svr19 6.32
Protein with the highest isoelectric point:
>tr|T1YRW5|T1YRW5_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 5 OX=1391034 PE=4 SV=1
MM1 pKa = 7.78 AYY3 pKa = 9.79 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 10.07 SYY8 pKa = 10.46 SSRR11 pKa = 11.84 ARR13 pKa = 11.84 RR14 pKa = 11.84 PIGRR18 pKa = 11.84 SAKK21 pKa = 10.01 RR22 pKa = 11.84 GGARR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 5.34 PTKK32 pKa = 10.21 KK33 pKa = 8.56 RR34 pKa = 11.84 TYY36 pKa = 8.62 RR37 pKa = 11.84 KK38 pKa = 9.1 NRR40 pKa = 11.84 AMSTRR45 pKa = 11.84 TILNKK50 pKa = 9.28 TSRR53 pKa = 11.84 KK54 pKa = 8.91 KK55 pKa = 10.61 RR56 pKa = 11.84 NGMLTWANTSTSGTSIPNVATNYY79 pKa = 10.18 FLTGNLGGRR88 pKa = 11.84 SIWCATAQDD97 pKa = 4.27 LTVQDD102 pKa = 4.27 GSFGTVALAAARR114 pKa = 11.84 TSTSCYY120 pKa = 8.69 MKK122 pKa = 10.59 GLSEE126 pKa = 5.06 HH127 pKa = 6.62 IRR129 pKa = 11.84 IQTSTGLPWFWRR141 pKa = 11.84 RR142 pKa = 11.84 ICFTLKK148 pKa = 10.67 GDD150 pKa = 3.82 DD151 pKa = 3.83 LFRR154 pKa = 11.84 FSTSDD159 pKa = 3.31 TPTSTSNRR167 pKa = 11.84 YY168 pKa = 9.6 LDD170 pKa = 3.69 TTGGMQRR177 pKa = 11.84 LFFNEE182 pKa = 3.79 LNNAQSNTINNHH194 pKa = 5.19 DD195 pKa = 4.5 AIIFKK200 pKa = 9.9 GAKK203 pKa = 9.23 GIDD206 pKa = 3.34 WIDD209 pKa = 4.2 YY210 pKa = 9.29 IVAPIDD216 pKa = 3.37 TSRR219 pKa = 11.84 INLKK223 pKa = 9.81 YY224 pKa = 10.93 DD225 pKa = 2.84 KK226 pKa = 10.3 VTLLRR231 pKa = 11.84 SGNANGTIKK240 pKa = 10.19 EE241 pKa = 4.03 AKK243 pKa = 8.93 LWHH246 pKa = 6.09 GMNKK250 pKa = 9.33 NLQYY254 pKa = 11.48 DD255 pKa = 3.82 DD256 pKa = 5.66 DD257 pKa = 4.59 EE258 pKa = 6.84 SGDD261 pKa = 4.01 KK262 pKa = 10.86 EE263 pKa = 4.46 STSYY267 pKa = 11.12 TSVDD271 pKa = 3.4 SKK273 pKa = 11.23 IGMGDD278 pKa = 3.74 YY279 pKa = 10.75 FVMDD283 pKa = 3.84 MFYY286 pKa = 10.52 PGAGGAAADD295 pKa = 4.34 IILVQANSTLYY306 pKa = 9.1 WHH308 pKa = 7.05 EE309 pKa = 4.08 KK310 pKa = 9.32
Molecular weight: 34.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.236
IPC2_protein 9.502
IPC_protein 9.677
Toseland 10.101
ProMoST 9.853
Dawson 10.306
Bjellqvist 9.999
Wikipedia 10.496
Rodwell 10.657
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.101
DTASelect 9.999
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.218
Patrickios 10.175
IPC_peptide 10.335
IPC2_peptide 8.741
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
645
310
335
322.5
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.372 ± 0.433
1.395 ± 0.293
6.977 ± 0.582
3.721 ± 1.225
4.341 ± 0.544
8.527 ± 0.096
2.326 ± 0.711
6.202 ± 0.05
5.736 ± 0.491
6.977 ± 0.36
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.326 ± 0.396
5.116 ± 0.917
3.256 ± 0.685
2.481 ± 0.153
7.287 ± 0.755
7.287 ± 1.198
6.822 ± 2.403
4.186 ± 1.102
2.636 ± 0.259
4.031 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here