Circovirus-like genome DCCV-5
Average proteome isoelectric point is 7.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WHA9|A0A190WHA9_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-5 OX=1788445 PE=4 SV=1
MM1 pKa = 6.7 TTQLLTPGSTHH12 pKa = 6.42 SGHH15 pKa = 6.84 SIALPVGRR23 pKa = 11.84 SPLVPEE29 pKa = 4.06 HH30 pKa = 5.54 LTYY33 pKa = 10.62 RR34 pKa = 11.84 DD35 pKa = 3.73 TPSTLIRR42 pKa = 11.84 SPSPPLDD49 pKa = 4.47 DD50 pKa = 4.53 YY51 pKa = 11.74 LWDD54 pKa = 4.09 VTWSPPVGPDD64 pKa = 3.43 PNPEE68 pKa = 3.67 LTALRR73 pKa = 11.84 MAAFARR79 pKa = 11.84 EE80 pKa = 4.16 VSPPPMRR87 pKa = 11.84 TPGTGRR93 pKa = 11.84 SSVGRR98 pKa = 11.84 TPVPWLRR105 pKa = 11.84 RR106 pKa = 11.84 ASSTRR111 pKa = 11.84 FPLIYY116 pKa = 10.32 TSDD119 pKa = 3.4 TSEE122 pKa = 4.03 TSTGSIEE129 pKa = 4.26 RR130 pKa = 11.84 FSRR133 pKa = 11.84 PWSPSPLPAGVGSWEE148 pKa = 4.07 EE149 pKa = 3.64 LDD151 pKa = 3.88 LVNLRR156 pKa = 11.84 EE157 pKa = 4.26 CDD159 pKa = 3.36 QPSRR163 pKa = 3.86
Molecular weight: 17.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.116
IPC2_protein 5.97
IPC_protein 6.071
Toseland 6.478
ProMoST 6.453
Dawson 6.351
Bjellqvist 6.287
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.634
Solomon 6.364
Lehninger 6.351
Nozaki 6.576
DTASelect 6.795
Thurlkill 6.81
EMBOSS 6.795
Sillero 6.722
Patrickios 4.584
IPC_peptide 6.376
IPC2_peptide 6.737
IPC2.peptide.svr19 6.58
Protein with the highest isoelectric point:
>tr|A0A190WHJ3|A0A190WHJ3_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-5 OX=1788445 PE=4 SV=1
MM1 pKa = 7.42 AAYY4 pKa = 9.41 AARR7 pKa = 11.84 IGYY10 pKa = 9.3 GLARR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 9.08 AVPAIRR22 pKa = 11.84 RR23 pKa = 11.84 SIPAIQRR30 pKa = 11.84 FARR33 pKa = 11.84 RR34 pKa = 11.84 SFVPRR39 pKa = 11.84 QNGLGIPLRR48 pKa = 11.84 RR49 pKa = 11.84 IAPSRR54 pKa = 11.84 AANFNQIRR62 pKa = 11.84 RR63 pKa = 11.84 MGSTAFPRR71 pKa = 11.84 TPTSNPIPSTSRR83 pKa = 11.84 AINRR87 pKa = 11.84 IARR90 pKa = 11.84 GATAAAATGGALKK103 pKa = 10.62 ALSNAKK109 pKa = 9.32 KK110 pKa = 10.38 RR111 pKa = 11.84 KK112 pKa = 9.87 AEE114 pKa = 4.03 EE115 pKa = 3.49 QNKK118 pKa = 9.35 RR119 pKa = 11.84 VKK121 pKa = 10.57 KK122 pKa = 10.52 DD123 pKa = 3.08 IEE125 pKa = 3.99 QNAASGLGDD134 pKa = 4.04 DD135 pKa = 4.75 VKK137 pKa = 10.6 TMDD140 pKa = 4.53 SFTHH144 pKa = 6.63 HH145 pKa = 6.88 SGNDD149 pKa = 3.1 SWLKK153 pKa = 9.98 AHH155 pKa = 6.83 LVSGKK160 pKa = 8.44 HH161 pKa = 4.31 TVKK164 pKa = 10.84 NLAFSNNLLGTQGRR178 pKa = 11.84 ARR180 pKa = 11.84 WFTVTTTQTPDD191 pKa = 3.05 DD192 pKa = 4.24 YY193 pKa = 10.99 RR194 pKa = 11.84 TLFQQTTEE202 pKa = 4.2 NYY204 pKa = 9.58 RR205 pKa = 11.84 STSTASATPSWVRR218 pKa = 11.84 GDD220 pKa = 4.26 PLLQSGYY227 pKa = 10.37 LNQKK231 pKa = 9.38 FVYY234 pKa = 9.54 EE235 pKa = 4.11 KK236 pKa = 10.97 LKK238 pKa = 11.1 ANYY241 pKa = 9.04 IFKK244 pKa = 10.33 NQGSSPVHH252 pKa = 5.12 MEE254 pKa = 3.67 YY255 pKa = 10.61 FIITPKK261 pKa = 9.54 DD262 pKa = 3.35 TNEE265 pKa = 4.68 IARR268 pKa = 11.84 NWQNDD273 pKa = 3.75 YY274 pKa = 11.8 VNGLSDD280 pKa = 3.67 STAEE284 pKa = 4.19 LAVKK288 pKa = 10.47 ASISSGVLVTPCEE301 pKa = 4.11 YY302 pKa = 10.7 KK303 pKa = 10.27 IGSSPRR309 pKa = 11.84 FNKK312 pKa = 9.06 YY313 pKa = 7.22 WKK315 pKa = 9.36 ILYY318 pKa = 7.86 SHH320 pKa = 7.5 KK321 pKa = 10.72 VRR323 pKa = 11.84 MAEE326 pKa = 4.08 GAEE329 pKa = 4.14 HH330 pKa = 6.79 EE331 pKa = 5.51 FNFTDD336 pKa = 3.27 NANRR340 pKa = 11.84 EE341 pKa = 4.28 IQWNGFPVNGGQLKK355 pKa = 10.57 GMTKK359 pKa = 9.75 YY360 pKa = 10.62 FIVKK364 pKa = 9.72 IYY366 pKa = 10.95 GDD368 pKa = 3.82 IGDD371 pKa = 4.24 SSNLLGTIGTITTAPAKK388 pKa = 10.24 IIGILRR394 pKa = 11.84 HH395 pKa = 5.55 TEE397 pKa = 4.13 TVRR400 pKa = 11.84 HH401 pKa = 4.52 VHH403 pKa = 5.59 RR404 pKa = 11.84 TPSLFLDD411 pKa = 5.3 FSADD415 pKa = 3.47 LATNAAALYY424 pKa = 9.38 EE425 pKa = 4.3 KK426 pKa = 10.72 DD427 pKa = 3.32 GGTGKK432 pKa = 10.24 VDD434 pKa = 3.41 NVLLEE439 pKa = 4.16 VQPP442 pKa = 4.25
Molecular weight: 48.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.516
IPC_protein 9.692
Toseland 10.204
ProMoST 9.882
Dawson 10.379
Bjellqvist 10.043
Wikipedia 10.555
Rodwell 10.789
Grimsley 10.452
Solomon 10.409
Lehninger 10.379
Nozaki 10.175
DTASelect 10.043
Thurlkill 10.233
EMBOSS 10.599
Sillero 10.292
Patrickios 10.394
IPC_peptide 10.409
IPC2_peptide 8.639
IPC2.peptide.svr19 8.645
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1034
148
442
258.5
29.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.544 ± 1.338
0.774 ± 0.304
4.739 ± 0.41
4.932 ± 0.64
3.385 ± 0.425
7.06 ± 0.441
2.611 ± 0.454
5.222 ± 0.888
4.739 ± 1.102
8.414 ± 0.892
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.451 ± 0.311
4.739 ± 1.12
7.06 ± 1.923
3.675 ± 1.004
8.027 ± 0.404
8.027 ± 1.221
7.35 ± 0.959
4.545 ± 0.544
2.224 ± 0.398
3.482 ± 0.557
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here