Beihai sobemo-like virus 22

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEB1|A0A1L3KEB1_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 22 OX=1922694 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 4.36WNSSPGVEE10 pKa = 4.0LEE12 pKa = 4.82KK13 pKa = 11.23YY14 pKa = 10.54GSTNRR19 pKa = 11.84QVWQVEE25 pKa = 4.1NGVPDD30 pKa = 4.21PQQKK34 pKa = 10.78LLMWKK39 pKa = 10.03IIQEE43 pKa = 4.35RR44 pKa = 11.84IKK46 pKa = 10.24EE47 pKa = 4.07LQIEE51 pKa = 4.36PTLGPVKK58 pKa = 10.42LFPKK62 pKa = 10.21YY63 pKa = 9.74EE64 pKa = 3.71PHH66 pKa = 6.91KK67 pKa = 9.89ISKK70 pKa = 9.56IRR72 pKa = 11.84EE73 pKa = 3.67NRR75 pKa = 11.84EE76 pKa = 3.39RR77 pKa = 11.84LIFGVGAVDD86 pKa = 4.15NIIAAMFFRR95 pKa = 11.84NWTAALVEE103 pKa = 4.34KK104 pKa = 9.98WRR106 pKa = 11.84EE107 pKa = 3.54IPIKK111 pKa = 10.5VGYY114 pKa = 9.83SPSQGGYY121 pKa = 9.05YY122 pKa = 10.51DD123 pKa = 3.88LFSYY127 pKa = 10.53VSRR130 pKa = 11.84LTNKK134 pKa = 8.2FTAADD139 pKa = 3.54KK140 pKa = 11.02SQWDD144 pKa = 3.69WSCQEE149 pKa = 3.79WEE151 pKa = 4.01VDD153 pKa = 3.44VARR156 pKa = 11.84QVLQHH161 pKa = 6.78LGDD164 pKa = 4.36FEE166 pKa = 7.1DD167 pKa = 4.77GTIADD172 pKa = 4.23NIIRR176 pKa = 11.84AHH178 pKa = 6.86FGPATLVLGKK188 pKa = 10.63AEE190 pKa = 4.18IEE192 pKa = 4.17KK193 pKa = 10.46KK194 pKa = 10.58GYY196 pKa = 10.71GVMFSGTYY204 pKa = 7.79FTLTFNSLFQLILHH218 pKa = 6.73EE219 pKa = 4.59AACVMIGRR227 pKa = 11.84EE228 pKa = 4.0YY229 pKa = 10.84AAPACIGDD237 pKa = 3.95DD238 pKa = 3.63TVQPRR243 pKa = 11.84CEE245 pKa = 4.86DD246 pKa = 3.35EE247 pKa = 4.55QYY249 pKa = 10.51WNAIRR254 pKa = 11.84GFGHH258 pKa = 6.5KK259 pKa = 10.34LKK261 pKa = 10.71DD262 pKa = 3.36IVHH265 pKa = 5.6TTDD268 pKa = 2.73EE269 pKa = 4.75FEE271 pKa = 4.4FAGHH275 pKa = 6.58RR276 pKa = 11.84VTNKK280 pKa = 10.01GAWPVYY286 pKa = 9.5LGKK289 pKa = 9.92HH290 pKa = 4.97RR291 pKa = 11.84AQLQFLTDD299 pKa = 3.52EE300 pKa = 4.49PEE302 pKa = 3.74IRR304 pKa = 11.84MMTLASYY311 pKa = 10.2QRR313 pKa = 11.84LYY315 pKa = 11.28ACDD318 pKa = 3.81ADD320 pKa = 4.2RR321 pKa = 11.84LKK323 pKa = 10.29TFTMALRR330 pKa = 11.84GSQYY334 pKa = 10.34YY335 pKa = 10.5SSSQEE340 pKa = 3.27IRR342 pKa = 11.84NWYY345 pKa = 9.16HH346 pKa = 4.95GWEE349 pKa = 4.13SAANQEE355 pKa = 4.62TAWW358 pKa = 3.65

Molecular weight: 41.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEB1|A0A1L3KEB1_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 22 OX=1922694 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.52NVLLVLLVVRR12 pKa = 11.84VVVCPNLTLDD22 pKa = 4.21DD23 pKa = 4.53LRR25 pKa = 11.84PIPEE29 pKa = 4.06QLMNMTVDD37 pKa = 2.77AWKK40 pKa = 10.85VYY42 pKa = 7.49MAYY45 pKa = 10.27YY46 pKa = 10.22RR47 pKa = 11.84SPAFNVVRR55 pKa = 11.84CYY57 pKa = 10.97GPWVTFMITVGACAIALLKK76 pKa = 10.42EE77 pKa = 4.14RR78 pKa = 11.84EE79 pKa = 4.11ARR81 pKa = 11.84EE82 pKa = 3.95AKK84 pKa = 10.44AKK86 pKa = 10.56LEE88 pKa = 3.97RR89 pKa = 11.84LFKK92 pKa = 10.58TMHH95 pKa = 5.9TVNGSLRR102 pKa = 11.84CPVLTPEE109 pKa = 4.56SLRR112 pKa = 11.84QGSEE116 pKa = 2.87ISRR119 pKa = 11.84NVKK122 pKa = 9.81KK123 pKa = 11.18VPGTFAVAMKK133 pKa = 10.41LAGEE137 pKa = 4.24LTVVGYY143 pKa = 9.67GMRR146 pKa = 11.84LEE148 pKa = 4.29NAAVFPLHH156 pKa = 6.69LCGDD160 pKa = 4.25SEE162 pKa = 5.47DD163 pKa = 3.9GTITIMNNGKK173 pKa = 9.77NGLKK177 pKa = 9.25TATLEE182 pKa = 4.08YY183 pKa = 9.49SAHH186 pKa = 6.24VEE188 pKa = 3.6VDD190 pKa = 2.86TDD192 pKa = 3.55VGVWQKK198 pKa = 7.66TTDD201 pKa = 3.49FWSQLEE207 pKa = 4.21VGKK210 pKa = 10.89ANIGILSAPAMAQAIAGGKK229 pKa = 8.91MSMGTLKK236 pKa = 10.57NDD238 pKa = 3.55PEE240 pKa = 4.5VVGRR244 pKa = 11.84LEE246 pKa = 4.14YY247 pKa = 10.78AGSTEE252 pKa = 4.22AGFSGGAYY260 pKa = 10.01LVATQIVGMHH270 pKa = 6.21FSGGSVNKK278 pKa = 10.12GFALMYY284 pKa = 9.6LYY286 pKa = 11.11SLIRR290 pKa = 11.84SVFNQEE296 pKa = 3.86AGSDD300 pKa = 4.06YY301 pKa = 11.31SWMNALVKK309 pKa = 10.56RR310 pKa = 11.84KK311 pKa = 9.72VKK313 pKa = 10.1VRR315 pKa = 11.84ARR317 pKa = 11.84RR318 pKa = 11.84HH319 pKa = 4.24PTNRR323 pKa = 11.84DD324 pKa = 3.16EE325 pKa = 6.62VIMQYY330 pKa = 10.32EE331 pKa = 4.56GQYY334 pKa = 10.86HH335 pKa = 6.09LVPYY339 pKa = 10.12EE340 pKa = 4.17EE341 pKa = 4.33FSMRR345 pKa = 11.84YY346 pKa = 8.12GALGAGEE353 pKa = 4.14TDD355 pKa = 3.61FVGRR359 pKa = 11.84EE360 pKa = 4.07VVPEE364 pKa = 4.33VYY366 pKa = 9.66TGHH369 pKa = 6.96TDD371 pKa = 3.95NIAGDD376 pKa = 3.92LAFNTAVPLNYY387 pKa = 10.04DD388 pKa = 3.72NPQVTPGGPHH398 pKa = 5.11RR399 pKa = 11.84TKK401 pKa = 10.61NAEE404 pKa = 4.13NVEE407 pKa = 4.17KK408 pKa = 9.48TLQMNALEE416 pKa = 4.47LQLSKK421 pKa = 10.92LRR423 pKa = 11.84EE424 pKa = 3.89KK425 pKa = 11.3YY426 pKa = 9.55EE427 pKa = 4.53AIVRR431 pKa = 11.84ATEE434 pKa = 3.99KK435 pKa = 10.5FVAQYY440 pKa = 10.89QPDD443 pKa = 3.95PKK445 pKa = 10.89AKK447 pKa = 10.63EE448 pKa = 3.54EE449 pKa = 4.01DD450 pKa = 3.19ALLRR454 pKa = 11.84GKK456 pKa = 9.18MKK458 pKa = 10.36QAAQEE463 pKa = 3.89ITAEE467 pKa = 3.83IHH469 pKa = 6.63KK470 pKa = 10.61EE471 pKa = 3.63MEE473 pKa = 4.38AVSKK477 pKa = 10.97SLTEE481 pKa = 3.82VRR483 pKa = 11.84RR484 pKa = 11.84NTDD487 pKa = 2.88EE488 pKa = 4.3EE489 pKa = 3.83IRR491 pKa = 11.84NRR493 pKa = 11.84RR494 pKa = 11.84RR495 pKa = 11.84AKK497 pKa = 9.04KK498 pKa = 7.9TVRR501 pKa = 11.84KK502 pKa = 8.86LASNEE507 pKa = 3.74DD508 pKa = 3.56VVRR511 pKa = 11.84NSLQRR516 pKa = 11.84VPDD519 pKa = 4.42FVQCHH524 pKa = 5.88FGDD527 pKa = 4.06LPTEE531 pKa = 3.88LRR533 pKa = 11.84DD534 pKa = 3.65RR535 pKa = 11.84LKK537 pKa = 11.21DD538 pKa = 3.41STTSILKK545 pKa = 10.43LKK547 pKa = 10.78VPTTSTSSQRR557 pKa = 11.84SPEE560 pKa = 3.85NDD562 pKa = 3.25TPLPSAGTPLSPPSS576 pKa = 3.39

Molecular weight: 63.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

934

358

576

467.0

52.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.244 ± 0.234

1.178 ± 0.121

4.497 ± 0.295

7.388 ± 0.086

3.533 ± 0.674

6.959 ± 0.013

2.034 ± 0.266

4.283 ± 1.033

5.996 ± 0.227

8.351 ± 0.604

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.423

4.39 ± 0.421

4.604 ± 0.384

4.176 ± 0.782

5.996 ± 0.227

5.46 ± 0.24

6.317 ± 0.56

7.923 ± 1.296

1.927 ± 0.945

3.747 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski