Enterococcus phage vB_EfaS_LM99

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H2F4|A0A2Z5H2F4_9CAUD Putative membrane protein OS=Enterococcus phage vB_EfaS_LM99 OX=2234084 GN=LM99_0053 PE=4 SV=1
MM1 pKa = 7.38YY2 pKa = 9.69IVNIIDD8 pKa = 3.8FEE10 pKa = 4.85TMVLAYY16 pKa = 9.71QSEE19 pKa = 4.71VFTTKK24 pKa = 10.32EE25 pKa = 3.5LAEE28 pKa = 4.79DD29 pKa = 3.76YY30 pKa = 10.11MGEE33 pKa = 4.24VMEE36 pKa = 4.72NTHH39 pKa = 6.54GLDD42 pKa = 3.31GLIIEE47 pKa = 4.52VSSTNLEE54 pKa = 3.9TAKK57 pKa = 10.35IVAHH61 pKa = 5.75SS62 pKa = 3.57

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H2D9|A0A2Z5H2D9_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_LM99 OX=2234084 GN=LM99_0039 PE=4 SV=1
MM1 pKa = 7.5VPFQLYY7 pKa = 6.98QTFHH11 pKa = 7.1RR12 pKa = 11.84LLHH15 pKa = 6.45RR16 pKa = 11.84FTITNYY22 pKa = 9.64FPHH25 pKa = 6.63KK26 pKa = 8.0QHH28 pKa = 6.82KK29 pKa = 9.03NWFLKK34 pKa = 9.89QHH36 pKa = 4.77VLCYY40 pKa = 10.34NFVRR44 pKa = 11.84EE45 pKa = 4.49FFLNLNYY52 pKa = 10.76SKK54 pKa = 11.13GGNYY58 pKa = 9.9YY59 pKa = 10.74SSTKK63 pKa = 10.25ANHH66 pKa = 6.43GKK68 pKa = 10.06RR69 pKa = 11.84FAHH72 pKa = 6.11ATNTCVQVANRR83 pKa = 11.84SRR85 pKa = 3.19

Molecular weight: 10.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12165

50

1456

190.1

21.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.902 ± 0.452

0.625 ± 0.115

6.091 ± 0.215

8.023 ± 0.519

4.332 ± 0.207

6.617 ± 0.634

1.562 ± 0.175

6.872 ± 0.202

8.878 ± 0.337

8.187 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.184

6.363 ± 0.295

2.803 ± 0.209

3.79 ± 0.2

3.485 ± 0.189

5.228 ± 0.297

6.28 ± 0.35

6.7 ± 0.234

1.299 ± 0.121

4.053 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski