Halorhabdus sp. CBA1104
Average proteome isoelectric point is 4.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2453 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A650A552|A0A650A552_9EURY 30S ribosomal protein S15 OS=Halorhabdus sp. CBA1104 OX=1380432 GN=rps15 PE=3 SV=1
MM1 pKa = 7.86 DD2 pKa = 5.89 RR3 pKa = 11.84 NPGLWLAVSIIVGIFLAVQLGALALVEE30 pKa = 4.08 PLKK33 pKa = 11.06 GAGLQPVEE41 pKa = 5.21 DD42 pKa = 4.5 PQDD45 pKa = 3.65 PTNSLLYY52 pKa = 10.06 ILAILVMTGVMLAAFRR68 pKa = 11.84 YY69 pKa = 8.13 NGEE72 pKa = 3.57 WAIRR76 pKa = 11.84 GLIVLTGVYY85 pKa = 10.01 ISWLVFSILVPPVVTLPVAGGVHH108 pKa = 5.6 VLAGAGAIAIGGGLLVYY125 pKa = 9.25 PEE127 pKa = 3.78 WWVIDD132 pKa = 3.21 IAGAVMGAGAAGLFGITFGVLPALVLLTVLAVYY165 pKa = 9.96 DD166 pKa = 4.71 AISVYY171 pKa = 9.27 GTEE174 pKa = 4.41 HH175 pKa = 6.88 MLTLAEE181 pKa = 4.64 GVMDD185 pKa = 4.65 LNVPVVLVAPTTLGYY200 pKa = 10.92 SFIDD204 pKa = 4.18 DD205 pKa = 5.0 DD206 pKa = 5.01 GPMDD210 pKa = 3.71 GTEE213 pKa = 4.02 SAEE216 pKa = 4.34 TEE218 pKa = 4.13 TAQQNSEE225 pKa = 4.75 DD226 pKa = 4.14 GDD228 pKa = 3.88 TDD230 pKa = 4.61 AEE232 pKa = 4.33 DD233 pKa = 4.35 ASFDD237 pKa = 4.23 DD238 pKa = 4.99 RR239 pKa = 11.84 DD240 pKa = 3.57 ALFIGLGDD248 pKa = 3.66 AVVPTVLVASAAFFAPASVPTVGIGGFTTVLPAATAMVGTFLGLAVLLWMVLKK301 pKa = 10.74 GRR303 pKa = 11.84 AHH305 pKa = 7.15 AGLPLLNGGVIGGYY319 pKa = 10.43 LLGALASGLTLLEE332 pKa = 4.28 ALGLAPYY339 pKa = 10.21 LL340 pKa = 4.06
Molecular weight: 34.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.554
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.884
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A650A2S1|A0A650A2S1_9EURY FAD-dependent oxidoreductase OS=Halorhabdus sp. CBA1104 OX=1380432 GN=Hrd1104_04460 PE=4 SV=1
MM1 pKa = 7.43 TLRR4 pKa = 11.84 LEE6 pKa = 4.4 GRR8 pKa = 11.84 SVSGDD13 pKa = 3.28 QFRR16 pKa = 11.84 AASVRR21 pKa = 11.84 AGSRR25 pKa = 11.84 ANAFHH30 pKa = 6.64 SFEE33 pKa = 4.42 RR34 pKa = 11.84 YY35 pKa = 9.2 SVEE38 pKa = 4.21 TFSSSCLARR47 pKa = 11.84 VATKK51 pKa = 10.38 LRR53 pKa = 11.84 AGNLGSAQMLWKK65 pKa = 10.63 YY66 pKa = 10.87 NYY68 pKa = 7.05 QTRR71 pKa = 11.84 LRR73 pKa = 11.84 TTTLTYY79 pKa = 10.73 HH80 pKa = 7.01 LADD83 pKa = 3.7 SQSTFLIRR91 pKa = 11.84 VIFLGAQKK99 pKa = 9.49 TLLHH103 pKa = 6.93 PDD105 pKa = 3.85 CPVVSPSGGNMFKK118 pKa = 10.86 VVGG121 pKa = 3.8
Molecular weight: 13.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.765
IPC_protein 10.628
Toseland 10.511
ProMoST 10.292
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.818
Grimsley 10.745
Solomon 10.76
Lehninger 10.716
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.599
Patrickios 10.57
IPC_peptide 10.76
IPC2_peptide 9.575
IPC2.peptide.svr19 8.39
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2453
0
2453
727833
44
2571
296.7
32.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.897 ± 0.075
0.658 ± 0.014
8.299 ± 0.066
8.554 ± 0.073
3.259 ± 0.034
8.35 ± 0.057
1.976 ± 0.022
4.523 ± 0.032
1.794 ± 0.025
8.94 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.754 ± 0.024
2.293 ± 0.032
4.657 ± 0.03
2.977 ± 0.03
6.347 ± 0.051
5.479 ± 0.038
6.711 ± 0.047
8.804 ± 0.05
1.114 ± 0.02
2.614 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here