Salehabad virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Salehabad phlebovirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0GAZ6|L0GAZ6_9VIRU Polyprotein OS=Salehabad virus OX=904699 PE=4 SV=1
TT1 pKa = 6.23KK2 pKa = 10.03TPYY5 pKa = 10.63CFIQTITMSLHH16 pKa = 5.06YY17 pKa = 10.21LCDD20 pKa = 5.38RR21 pKa = 11.84ITPTYY26 pKa = 10.39DD27 pKa = 3.39YY28 pKa = 9.79PCLGEE33 pKa = 4.06VMVSFEE39 pKa = 4.35AVNKK43 pKa = 9.65EE44 pKa = 3.74VGGPVCVYY52 pKa = 11.17DD53 pKa = 4.3DD54 pKa = 4.99LEE56 pKa = 5.4FEE58 pKa = 4.66LFEE61 pKa = 4.48SRR63 pKa = 11.84QSLSYY68 pKa = 11.05RR69 pKa = 11.84NTLYY73 pKa = 11.08DD74 pKa = 4.7FIDD77 pKa = 3.8SGEE80 pKa = 4.18LPWRR84 pKa = 11.84WGPHH88 pKa = 3.66MWGSRR93 pKa = 11.84VTLDD97 pKa = 4.05EE98 pKa = 5.29LPLLDD103 pKa = 5.04SLMKK107 pKa = 9.94TFQGLPVSDD116 pKa = 4.55LMDD119 pKa = 3.65SRR121 pKa = 11.84LHH123 pKa = 4.44YY124 pKa = 9.53TRR126 pKa = 11.84NSLIWPTGHH135 pKa = 6.49VSLKK139 pKa = 10.35AFRR142 pKa = 11.84ILYY145 pKa = 8.55PCIFVSFSYY154 pKa = 10.43SRR156 pKa = 11.84RR157 pKa = 11.84EE158 pKa = 3.38LAEE161 pKa = 4.09YY162 pKa = 10.15IFEE165 pKa = 4.34VTKK168 pKa = 10.75APHH171 pKa = 6.08LEE173 pKa = 3.93KK174 pKa = 10.83GIVDD178 pKa = 3.27MAGRR182 pKa = 11.84IRR184 pKa = 11.84SMAHH188 pKa = 5.26SLGIQEE194 pKa = 4.97NLVPCKK200 pKa = 11.11DD201 pKa = 3.54MMKK204 pKa = 10.16SICILQFFRR213 pKa = 11.84MIRR216 pKa = 11.84GFQRR220 pKa = 11.84DD221 pKa = 3.39RR222 pKa = 11.84TRR224 pKa = 11.84SVLFGGTVIPFFQACIDD241 pKa = 3.43ALTRR245 pKa = 11.84DD246 pKa = 4.17FPGLLDD252 pKa = 3.5VWSPGADD259 pKa = 2.59IFDD262 pKa = 5.98AITPSPCDD270 pKa = 3.22SGVSTDD276 pKa = 4.45SEE278 pKa = 4.22FDD280 pKa = 3.16SDD282 pKa = 4.69IEE284 pKa = 4.3VV285 pKa = 3.14

Molecular weight: 32.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0GCI2|L0GCI2_9VIRU Replicase OS=Salehabad virus OX=904699 PE=4 SV=1
MM1 pKa = 7.57SGQEE5 pKa = 4.1EE6 pKa = 4.18FAKK9 pKa = 10.48IAVEE13 pKa = 4.25FANEE17 pKa = 4.02AVDD20 pKa = 3.51TAAILEE26 pKa = 4.32IVKK29 pKa = 9.88EE30 pKa = 4.17FAYY33 pKa = 10.15QGYY36 pKa = 9.05DD37 pKa = 2.88AARR40 pKa = 11.84VVEE43 pKa = 4.13LVRR46 pKa = 11.84EE47 pKa = 4.23KK48 pKa = 11.35GGDD51 pKa = 2.92TWRR54 pKa = 11.84NDD56 pKa = 2.98VKK58 pKa = 10.33TLIIIALTRR67 pKa = 11.84GNKK70 pKa = 6.23PTKK73 pKa = 9.71ILEE76 pKa = 4.37KK77 pKa = 9.66MSPAGKK83 pKa = 9.93SKK85 pKa = 10.12FRR87 pKa = 11.84ALVLKK92 pKa = 10.8YY93 pKa = 10.04SLKK96 pKa = 10.59SGNPGRR102 pKa = 11.84DD103 pKa = 3.46DD104 pKa = 3.39LTLARR109 pKa = 11.84IASAFAAWTIQAIKK123 pKa = 10.56VVEE126 pKa = 4.26QYY128 pKa = 11.5LPVTGAAMDD137 pKa = 4.4EE138 pKa = 4.55LSSSYY143 pKa = 9.23PRR145 pKa = 11.84PMMHH149 pKa = 7.01PCFGGLIDD157 pKa = 3.73NTLPEE162 pKa = 4.31EE163 pKa = 4.17TVAALVRR170 pKa = 11.84AHH172 pKa = 6.95SLFLDD177 pKa = 3.88TFSRR181 pKa = 11.84TINPAMRR188 pKa = 11.84TKK190 pKa = 10.42TKK192 pKa = 11.12AEE194 pKa = 3.89VLKK197 pKa = 11.18SFEE200 pKa = 4.21QPLNAAINSRR210 pKa = 11.84FLTSEE215 pKa = 4.4AKK217 pKa = 10.25RR218 pKa = 11.84KK219 pKa = 8.68ILKK222 pKa = 9.76SAGVIDD228 pKa = 4.51TNLKK232 pKa = 9.76PSPAVKK238 pKa = 9.99QAADD242 pKa = 3.77KK243 pKa = 10.85FSEE246 pKa = 4.5TAA248 pKa = 3.36

Molecular weight: 27.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3966

248

2096

991.5

111.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.606 ± 1.039

2.597 ± 0.957

5.497 ± 0.507

6.531 ± 0.609

4.438 ± 0.58

5.925 ± 0.415

2.32 ± 0.313

6.278 ± 0.116

5.799 ± 0.54

9.228 ± 0.12

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.454 ± 0.472

3.454 ± 0.399

4.387 ± 0.358

2.799 ± 0.118

5.371 ± 0.272

9.002 ± 0.527

5.799 ± 0.436

6.354 ± 0.295

1.286 ± 0.093

2.874 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski