Clostridium phage phiCT453B
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7RTJ3|A0A0A7RTJ3_9CAUD Uncharacterized protein OS=Clostridium phage phiCT453B OX=1567013 GN=phiCT453B_13 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.74 NLPDD6 pKa = 3.55 CQYY9 pKa = 10.65 EE10 pKa = 4.05 YY11 pKa = 10.93 GYY13 pKa = 9.96 EE14 pKa = 4.07 SPIMQIAEE22 pKa = 3.91 EE23 pKa = 4.25 CSICGHH29 pKa = 6.73 EE30 pKa = 4.41 IYY32 pKa = 10.71 EE33 pKa = 4.37 GDD35 pKa = 3.67 GYY37 pKa = 10.59 YY38 pKa = 10.56 HH39 pKa = 7.38 IDD41 pKa = 3.59 NLNICEE47 pKa = 4.22 DD48 pKa = 4.56 CILDD52 pKa = 3.72 FKK54 pKa = 11.29 KK55 pKa = 10.4 IGEE58 pKa = 4.07 II59 pKa = 3.91
Molecular weight: 6.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.918
IPC2_protein 3.872
IPC_protein 3.757
Toseland 3.592
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.503
Solomon 3.719
Lehninger 3.668
Nozaki 3.884
DTASelect 4.012
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.884
Patrickios 0.006
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A0A7RTL0|A0A0A7RTL0_9CAUD Uncharacterized protein OS=Clostridium phage phiCT453B OX=1567013 GN=phiCT453B_07 PE=4 SV=1
MM1 pKa = 7.61 ALKK4 pKa = 10.24 KK5 pKa = 10.43 LCPKK9 pKa = 9.91 CGCLISMTEE18 pKa = 4.15 KK19 pKa = 10.71 LCQKK23 pKa = 10.27 CMPKK27 pKa = 10.18 YY28 pKa = 8.43 EE29 pKa = 4.09 QEE31 pKa = 4.1 KK32 pKa = 10.56 KK33 pKa = 10.54 EE34 pKa = 4.33 SNKK37 pKa = 10.59 LYY39 pKa = 10.51 DD40 pKa = 3.02 RR41 pKa = 11.84 RR42 pKa = 11.84 YY43 pKa = 8.1 RR44 pKa = 11.84 NKK46 pKa = 10.04 RR47 pKa = 11.84 STEE50 pKa = 4.27 FYY52 pKa = 10.41 HH53 pKa = 6.39 SKK55 pKa = 9.5 SWLIVRR61 pKa = 11.84 EE62 pKa = 4.1 QIRR65 pKa = 11.84 QRR67 pKa = 11.84 DD68 pKa = 3.48 NGLCKK73 pKa = 10.54 LCLRR77 pKa = 11.84 NKK79 pKa = 10.06 KK80 pKa = 8.95 IRR82 pKa = 11.84 SMDD85 pKa = 3.44 MVHH88 pKa = 7.3 HH89 pKa = 6.35 IEE91 pKa = 4.17 EE92 pKa = 4.52 LRR94 pKa = 11.84 EE95 pKa = 4.07 KK96 pKa = 10.48 YY97 pKa = 10.15 NKK99 pKa = 10.1 RR100 pKa = 11.84 LGKK103 pKa = 10.36 DD104 pKa = 3.33 NLICLCNKK112 pKa = 9.04 CHH114 pKa = 6.27 NAVHH118 pKa = 6.81 AEE120 pKa = 3.82 YY121 pKa = 11.26 SNGNKK126 pKa = 10.35 ASMQEE131 pKa = 3.92 EE132 pKa = 4.45 LRR134 pKa = 11.84 RR135 pKa = 11.84 LIDD138 pKa = 3.27 RR139 pKa = 11.84 RR140 pKa = 11.84 GYY142 pKa = 10.34
Molecular weight: 17.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.17
IPC2_protein 8.916
IPC_protein 8.829
Toseland 9.633
ProMoST 9.399
Dawson 9.867
Bjellqvist 9.633
Wikipedia 9.999
Rodwell 10.262
Grimsley 9.911
Solomon 9.897
Lehninger 9.867
Nozaki 9.867
DTASelect 9.545
Thurlkill 9.75
EMBOSS 10.058
Sillero 9.853
Patrickios 7.556
IPC_peptide 9.897
IPC2_peptide 8.565
IPC2.peptide.svr19 7.927
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
11078
39
859
181.6
20.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.019 ± 0.509
1.327 ± 0.18
5.831 ± 0.282
8.494 ± 0.446
3.647 ± 0.242
5.579 ± 0.33
1.083 ± 0.161
9.307 ± 0.248
11.284 ± 0.275
8.431 ± 0.306
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.582 ± 0.173
7.285 ± 0.372
2.23 ± 0.152
2.871 ± 0.147
3.782 ± 0.32
5.109 ± 0.232
5.497 ± 0.37
5.326 ± 0.346
1.101 ± 0.11
4.216 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here