Gordonia sihwensis NBRC 108236

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; Gordonia sihwensis

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3903 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7LGV2|L7LGV2_9ACTN 30S ribosomal protein S12 OS=Gordonia sihwensis NBRC 108236 OX=1223544 GN=rpsL PE=3 SV=1
MM1 pKa = 7.71SDD3 pKa = 3.71DD4 pKa = 3.77PLMVGDD10 pKa = 4.92CGDD13 pKa = 3.46VVAADD18 pKa = 5.04PYY20 pKa = 11.79ADD22 pKa = 3.06ICDD25 pKa = 3.46RR26 pKa = 11.84VTAYY30 pKa = 10.67VEE32 pKa = 4.2EE33 pKa = 4.17MLDD36 pKa = 3.41AYY38 pKa = 10.05FVAVLGHH45 pKa = 6.33SPTAIRR51 pKa = 11.84GDD53 pKa = 3.03GWLRR57 pKa = 11.84PLLGSYY63 pKa = 9.92EE64 pKa = 4.05LADD67 pKa = 3.85RR68 pKa = 11.84QGPP71 pKa = 3.54

Molecular weight: 7.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7LJR8|L7LJR8_9ACTN Glycerol kinase OS=Gordonia sihwensis NBRC 108236 OX=1223544 GN=glpK PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.76GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3903

0

3903

1261255

45

14427

323.2

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.142 ± 0.052

0.727 ± 0.012

6.636 ± 0.039

5.479 ± 0.041

2.892 ± 0.022

8.949 ± 0.034

2.065 ± 0.018

4.251 ± 0.031

2.212 ± 0.035

9.534 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.02

2.027 ± 0.021

5.564 ± 0.031

2.713 ± 0.024

7.618 ± 0.044

5.649 ± 0.027

6.089 ± 0.027

8.932 ± 0.038

1.429 ± 0.019

2.048 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski