Erinaceus europaeus papillomavirus 1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7TQP2|B7TQP2_9PAPI Minor capsid protein L2 OS=Erinaceus europaeus papillomavirus 1 OX=445217 GN=L2 PE=3 SV=1
MM1 pKa = 7.26 ARR3 pKa = 11.84 SRR5 pKa = 11.84 RR6 pKa = 11.84 TKK8 pKa = 10.16 RR9 pKa = 11.84 ASAQDD14 pKa = 3.87 LYY16 pKa = 11.2 KK17 pKa = 10.69 SCLSGGDD24 pKa = 3.86 CPPDD28 pKa = 3.34 VKK30 pKa = 10.92 NKK32 pKa = 10.19 YY33 pKa = 7.32 EE34 pKa = 4.07 QNTTADD40 pKa = 4.17 KK41 pKa = 10.18 ILKK44 pKa = 9.13 YY45 pKa = 10.75 GSTAVYY51 pKa = 10.09 FGGLGIGTGKK61 pKa = 8.82 GTGGVTGYY69 pKa = 8.86 TPLGGGEE76 pKa = 4.15 GPVRR80 pKa = 11.84 VGVPRR85 pKa = 11.84 VLRR88 pKa = 11.84 PALATDD94 pKa = 4.08 LVGPLDD100 pKa = 4.04 IAPVDD105 pKa = 3.79 AGVGANDD112 pKa = 4.01 PSVITLTDD120 pKa = 3.11 STLSVDD126 pKa = 3.6 VGPGEE131 pKa = 4.26 VEE133 pKa = 4.4 VVAEE137 pKa = 3.99 IHH139 pKa = 6.19 PVPDD143 pKa = 3.32 TSTTTTGSGQGTSAVLEE160 pKa = 4.22 ITPEE164 pKa = 3.79 ATPGKK169 pKa = 9.74 VRR171 pKa = 11.84 VSRR174 pKa = 11.84 TQYY177 pKa = 10.34 HH178 pKa = 5.77 NPAFHH183 pKa = 7.44 ILTTSTPIGGEE194 pKa = 3.8 TSAVDD199 pKa = 3.42 NVFVDD204 pKa = 4.89 FGSPSGHH211 pKa = 5.98 IVGADD216 pKa = 3.15 GVEE219 pKa = 4.34 VYY221 pKa = 10.82 EE222 pKa = 4.77 EE223 pKa = 4.42 IPLEE227 pKa = 3.92 PLNRR231 pKa = 11.84 SEE233 pKa = 5.98 FEE235 pKa = 4.32 IEE237 pKa = 3.91 EE238 pKa = 4.05 QTPKK242 pKa = 10.87 SSTPKK247 pKa = 10.35 ILDD250 pKa = 3.97 TIADD254 pKa = 4.16 SVRR257 pKa = 11.84 KK258 pKa = 9.53 FYY260 pKa = 11.07 NRR262 pKa = 11.84 RR263 pKa = 11.84 IQQIKK268 pKa = 7.94 TSDD271 pKa = 3.42 YY272 pKa = 10.93 RR273 pKa = 11.84 FLSSPGKK280 pKa = 10.39 LVDD283 pKa = 4.01 ASFINPAYY291 pKa = 10.59 DD292 pKa = 3.68 PDD294 pKa = 3.71 EE295 pKa = 4.23 TLVFDD300 pKa = 3.88 TDD302 pKa = 3.43 INEE305 pKa = 4.17 VQMAPNPDD313 pKa = 3.45 FQDD316 pKa = 2.64 IRR318 pKa = 11.84 VLNRR322 pKa = 11.84 PMFSTKK328 pKa = 9.67 EE329 pKa = 3.8 GYY331 pKa = 9.81 IRR333 pKa = 11.84 VSRR336 pKa = 11.84 LGNRR340 pKa = 11.84 GTIRR344 pKa = 11.84 TRR346 pKa = 11.84 SGTQIGAQVHH356 pKa = 6.09 FFQDD360 pKa = 3.77 LSSIDD365 pKa = 3.65 AEE367 pKa = 4.43 EE368 pKa = 4.3 GLEE371 pKa = 4.03 LALLGQHH378 pKa = 6.43 SGDD381 pKa = 3.45 ASIVQAQAEE390 pKa = 4.84 SVFLGDD396 pKa = 4.51 GLHH399 pKa = 7.4 PDD401 pKa = 3.42 TDD403 pKa = 4.72 SNLSLLDD410 pKa = 3.46 EE411 pKa = 4.45 FAEE414 pKa = 4.71 DD415 pKa = 4.6 FSHH418 pKa = 6.22 SQLIIGEE425 pKa = 4.17 RR426 pKa = 11.84 KK427 pKa = 9.46 ANQVSLPDD435 pKa = 3.84 FASPKK440 pKa = 7.8 YY441 pKa = 10.58 SKK443 pKa = 11.19 YY444 pKa = 10.5 FLQDD448 pKa = 3.1 YY449 pKa = 10.64 GNGFIVSHH457 pKa = 6.48 PMQTEE462 pKa = 3.92 RR463 pKa = 11.84 PEE465 pKa = 4.72 IIHH468 pKa = 6.95 PDD470 pKa = 2.91 IDD472 pKa = 3.95 AGPTIVIEE480 pKa = 4.58 AFDD483 pKa = 3.84 SSGGFYY489 pKa = 10.3 LHH491 pKa = 7.23 PSFVGKK497 pKa = 9.65 KK498 pKa = 9.07 RR499 pKa = 11.84 KK500 pKa = 9.34 RR501 pKa = 11.84 LYY503 pKa = 10.69 FLL505 pKa = 4.65
Molecular weight: 54.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.884
IPC2_protein 4.876
IPC_protein 4.838
Toseland 4.736
ProMoST 4.978
Dawson 4.825
Bjellqvist 4.952
Wikipedia 4.711
Rodwell 4.711
Grimsley 4.647
Solomon 4.825
Lehninger 4.774
Nozaki 4.94
DTASelect 5.13
Thurlkill 4.736
EMBOSS 4.736
Sillero 4.991
Patrickios 4.062
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 4.945
Protein with the highest isoelectric point:
>tr|B7TQP1|B7TQP1_9PAPI E4 (Fragment) OS=Erinaceus europaeus papillomavirus 1 OX=445217 GN=E4 PE=4 SV=1
MM1 pKa = 7.48 EE2 pKa = 6.16 RR3 pKa = 11.84 LTQRR7 pKa = 11.84 FDD9 pKa = 3.36 ALQEE13 pKa = 3.94 KK14 pKa = 10.44 LMEE17 pKa = 4.82 IYY19 pKa = 10.61 EE20 pKa = 4.32 KK21 pKa = 10.62 DD22 pKa = 3.66 SRR24 pKa = 11.84 DD25 pKa = 3.6 LGVMTSHH32 pKa = 7.53 WALQRR37 pKa = 11.84 EE38 pKa = 4.6 EE39 pKa = 4.09 QALLHH44 pKa = 5.55 CARR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 9.58 GVLRR53 pKa = 11.84 VGFQPVPPLKK63 pKa = 10.74 VSEE66 pKa = 4.44 QKK68 pKa = 10.92 AKK70 pKa = 10.75 AAIEE74 pKa = 3.94 MHH76 pKa = 6.32 LTLQSLQKK84 pKa = 10.15 SAYY87 pKa = 10.21 SNEE90 pKa = 3.87 DD91 pKa = 2.57 WTLSQTSRR99 pKa = 11.84 EE100 pKa = 4.19 SFMTPPRR107 pKa = 11.84 HH108 pKa = 5.78 CFKK111 pKa = 10.9 KK112 pKa = 9.91 QGHH115 pKa = 4.64 TVEE118 pKa = 4.25 VTFDD122 pKa = 3.74 GEE124 pKa = 4.63 SANSMLYY131 pKa = 9.2 TMWGRR136 pKa = 11.84 VYY138 pKa = 10.99 YY139 pKa = 10.93 QMDD142 pKa = 4.01 DD143 pKa = 4.01 GSWSVASSGVDD154 pKa = 3.19 YY155 pKa = 11.49 YY156 pKa = 11.47 GIYY159 pKa = 10.85 YY160 pKa = 9.91 NDD162 pKa = 3.87 SEE164 pKa = 4.82 GKK166 pKa = 7.3 PHH168 pKa = 6.73 YY169 pKa = 9.18 YY170 pKa = 10.67 VKK172 pKa = 10.45 FAEE175 pKa = 4.62 DD176 pKa = 3.16 AQKK179 pKa = 10.97 YY180 pKa = 9.54 SKK182 pKa = 9.8 TGTWFVRR189 pKa = 11.84 SNDD192 pKa = 3.47 KK193 pKa = 10.4 TISAPVTSTCTSPTTRR209 pKa = 11.84 DD210 pKa = 3.04 RR211 pKa = 11.84 SRR213 pKa = 11.84 SPRR216 pKa = 11.84 RR217 pKa = 11.84 HH218 pKa = 5.09 TEE220 pKa = 4.02 EE221 pKa = 4.12 VDD223 pKa = 3.29 STTPPGRR230 pKa = 11.84 RR231 pKa = 11.84 YY232 pKa = 9.67 PPSSPPSVSSLRR244 pKa = 11.84 LRR246 pKa = 11.84 GRR248 pKa = 11.84 GGGEE252 pKa = 3.69 GEE254 pKa = 4.35 YY255 pKa = 10.79 YY256 pKa = 10.21 PFGRR260 pKa = 11.84 SSPSEE265 pKa = 4.08 DD266 pKa = 3.06 RR267 pKa = 11.84 CGSAGSPARR276 pKa = 11.84 PVGRR280 pKa = 11.84 RR281 pKa = 11.84 PPHH284 pKa = 6.45 CPQGTPAEE292 pKa = 4.21 ASHH295 pKa = 6.54 RR296 pKa = 11.84 LLWGPRR302 pKa = 11.84 DD303 pKa = 3.67 PTVLVAKK310 pKa = 10.54 GDD312 pKa = 4.14 ANTLKK317 pKa = 10.4 CWRR320 pKa = 11.84 NRR322 pKa = 11.84 CRR324 pKa = 11.84 KK325 pKa = 6.46 THH327 pKa = 6.4 AGSFIAFSTTWQWCGDD343 pKa = 3.69 GNCRR347 pKa = 11.84 QGRR350 pKa = 11.84 HH351 pKa = 5.21 RR352 pKa = 11.84 LQILFSSSQQLDD364 pKa = 3.32 QFLGKK369 pKa = 10.54 VKK371 pKa = 10.16 VPKK374 pKa = 10.09 GVEE377 pKa = 3.83 VHH379 pKa = 6.96 RR380 pKa = 11.84 STFDD384 pKa = 3.2 GLL386 pKa = 3.71
Molecular weight: 43.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.848
IPC2_protein 8.756
IPC_protein 8.697
Toseland 9.194
ProMoST 9.136
Dawson 9.56
Bjellqvist 9.428
Wikipedia 9.75
Rodwell 9.706
Grimsley 9.619
Solomon 9.604
Lehninger 9.56
Nozaki 9.531
DTASelect 9.326
Thurlkill 9.414
EMBOSS 9.677
Sillero 9.575
Patrickios 4.749
IPC_peptide 9.589
IPC2_peptide 8.331
IPC2.peptide.svr19 7.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2282
54
597
326.0
36.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.135 ± 0.148
2.323 ± 0.76
6.442 ± 0.533
5.96 ± 0.422
4.426 ± 0.331
6.968 ± 0.986
1.884 ± 0.306
4.426 ± 0.594
5.741 ± 0.602
8.94 ± 0.996
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.753 ± 0.291
3.812 ± 0.931
5.434 ± 0.874
4.294 ± 0.196
6.004 ± 0.786
7.581 ± 0.968
6.573 ± 0.403
6.529 ± 0.382
1.446 ± 0.408
3.33 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here