Amino acid dipepetide frequency for Mycobacterium phage Wilkins

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.837AlaAla: 12.837 ± 1.106
0.692AlaCys: 0.692 ± 0.203
7.551AlaAsp: 7.551 ± 0.641
5.978AlaGlu: 5.978 ± 0.674
3.272AlaPhe: 3.272 ± 0.513
7.74AlaGly: 7.74 ± 0.795
1.133AlaHis: 1.133 ± 0.328
4.405AlaIle: 4.405 ± 0.547
4.279AlaLys: 4.279 ± 0.564
9.439AlaLeu: 9.439 ± 0.963
2.58AlaMet: 2.58 ± 0.447
2.706AlaAsn: 2.706 ± 0.524
5.034AlaPro: 5.034 ± 0.738
3.146AlaGln: 3.146 ± 0.457
5.852AlaArg: 5.852 ± 0.525
5.16AlaSer: 5.16 ± 0.666
6.481AlaThr: 6.481 ± 0.66
8.621AlaVal: 8.621 ± 0.724
1.951AlaTrp: 1.951 ± 0.325
2.517AlaTyr: 2.517 ± 0.389
0.0AlaXaa: 0.0 ± 0.0
Cys
0.818CysAla: 0.818 ± 0.238
0.063CysCys: 0.063 ± 0.067
0.315CysAsp: 0.315 ± 0.147
0.692CysGlu: 0.692 ± 0.202
0.126CysPhe: 0.126 ± 0.072
0.503CysGly: 0.503 ± 0.232
0.126CysHis: 0.126 ± 0.091
0.315CysIle: 0.315 ± 0.166
0.189CysLys: 0.189 ± 0.103
0.44CysLeu: 0.44 ± 0.197
0.126CysMet: 0.126 ± 0.094
0.189CysAsn: 0.189 ± 0.097
0.44CysPro: 0.44 ± 0.185
0.126CysGln: 0.126 ± 0.083
0.503CysArg: 0.503 ± 0.211
0.378CysSer: 0.378 ± 0.173
0.189CysThr: 0.189 ± 0.098
0.252CysVal: 0.252 ± 0.119
0.126CysTrp: 0.126 ± 0.082
0.189CysTyr: 0.189 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
6.418AspAla: 6.418 ± 0.63
0.44AspCys: 0.44 ± 0.163
4.971AspAsp: 4.971 ± 0.602
3.335AspGlu: 3.335 ± 0.504
2.391AspPhe: 2.391 ± 0.314
5.789AspGly: 5.789 ± 0.611
1.321AspHis: 1.321 ± 0.302
2.957AspIle: 2.957 ± 0.506
3.02AspLys: 3.02 ± 0.557
6.985AspLeu: 6.985 ± 0.735
1.196AspMet: 1.196 ± 0.231
1.888AspAsn: 1.888 ± 0.354
4.908AspPro: 4.908 ± 0.671
1.888AspGln: 1.888 ± 0.374
3.964AspArg: 3.964 ± 0.429
3.146AspSer: 3.146 ± 0.49
4.09AspThr: 4.09 ± 0.476
5.034AspVal: 5.034 ± 0.589
1.825AspTrp: 1.825 ± 0.332
1.951AspTyr: 1.951 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
6.733GluAla: 6.733 ± 0.755
0.189GluCys: 0.189 ± 0.137
4.342GluAsp: 4.342 ± 0.538
5.6GluGlu: 5.6 ± 0.62
1.951GluPhe: 1.951 ± 0.342
3.713GluGly: 3.713 ± 0.476
1.384GluHis: 1.384 ± 0.318
3.775GluIle: 3.775 ± 0.486
2.58GluLys: 2.58 ± 0.494
6.796GluLeu: 6.796 ± 0.677
1.51GluMet: 1.51 ± 0.325
1.447GluAsn: 1.447 ± 0.34
2.58GluPro: 2.58 ± 0.454
2.706GluGln: 2.706 ± 0.469
4.027GluArg: 4.027 ± 0.517
3.461GluSer: 3.461 ± 0.442
3.713GluThr: 3.713 ± 0.468
4.971GluVal: 4.971 ± 0.673
1.636GluTrp: 1.636 ± 0.36
2.391GluTyr: 2.391 ± 0.424
0.0GluXaa: 0.0 ± 0.0
Phe
2.454PheAla: 2.454 ± 0.388
0.252PheCys: 0.252 ± 0.149
2.957PheAsp: 2.957 ± 0.428
2.077PheGlu: 2.077 ± 0.334
0.252PhePhe: 0.252 ± 0.137
3.335PheGly: 3.335 ± 0.514
0.818PheHis: 0.818 ± 0.254
1.321PheIle: 1.321 ± 0.267
1.447PheLys: 1.447 ± 0.276
2.706PheLeu: 2.706 ± 0.44
0.692PheMet: 0.692 ± 0.224
1.07PheAsn: 1.07 ± 0.256
1.825PhePro: 1.825 ± 0.309
1.07PheGln: 1.07 ± 0.217
1.888PheArg: 1.888 ± 0.355
1.384PheSer: 1.384 ± 0.232
2.077PheThr: 2.077 ± 0.377
2.139PheVal: 2.139 ± 0.412
0.629PheTrp: 0.629 ± 0.171
0.881PheTyr: 0.881 ± 0.3
0.0PheXaa: 0.0 ± 0.0
Gly
6.67GlyAla: 6.67 ± 0.772
0.566GlyCys: 0.566 ± 0.205
5.286GlyAsp: 5.286 ± 0.525
4.594GlyGlu: 4.594 ± 0.68
2.328GlyPhe: 2.328 ± 0.393
7.362GlyGly: 7.362 ± 0.942
1.699GlyHis: 1.699 ± 0.353
4.216GlyIle: 4.216 ± 0.666
3.083GlyLys: 3.083 ± 0.506
8.306GlyLeu: 8.306 ± 0.687
1.447GlyMet: 1.447 ± 0.273
3.272GlyAsn: 3.272 ± 0.435
3.838GlyPro: 3.838 ± 0.569
2.328GlyGln: 2.328 ± 0.379
4.971GlyArg: 4.971 ± 0.553
5.6GlySer: 5.6 ± 0.654
4.845GlyThr: 4.845 ± 0.587
6.23GlyVal: 6.23 ± 0.618
2.454GlyTrp: 2.454 ± 0.366
2.832GlyTyr: 2.832 ± 0.387
0.0GlyXaa: 0.0 ± 0.0
His
1.51HisAla: 1.51 ± 0.328
0.0HisCys: 0.0 ± 0.0
1.007HisAsp: 1.007 ± 0.219
1.51HisGlu: 1.51 ± 0.334
0.818HisPhe: 0.818 ± 0.203
1.321HisGly: 1.321 ± 0.364
0.755HisHis: 0.755 ± 0.191
0.692HisIle: 0.692 ± 0.212
1.007HisLys: 1.007 ± 0.26
1.321HisLeu: 1.321 ± 0.315
0.252HisMet: 0.252 ± 0.135
0.315HisAsn: 0.315 ± 0.129
1.321HisPro: 1.321 ± 0.29
0.881HisGln: 0.881 ± 0.198
1.447HisArg: 1.447 ± 0.319
0.755HisSer: 0.755 ± 0.213
1.007HisThr: 1.007 ± 0.221
1.636HisVal: 1.636 ± 0.317
0.566HisTrp: 0.566 ± 0.152
0.818HisTyr: 0.818 ± 0.281
0.0HisXaa: 0.0 ± 0.0
Ile
6.355IleAla: 6.355 ± 0.759
0.252IleCys: 0.252 ± 0.122
3.272IleAsp: 3.272 ± 0.346
3.461IleGlu: 3.461 ± 0.461
0.881IlePhe: 0.881 ± 0.212
3.838IleGly: 3.838 ± 0.459
0.818IleHis: 0.818 ± 0.229
1.825IleIle: 1.825 ± 0.271
1.699IleLys: 1.699 ± 0.295
3.02IleLeu: 3.02 ± 0.356
0.755IleMet: 0.755 ± 0.177
1.762IleAsn: 1.762 ± 0.303
3.02IlePro: 3.02 ± 0.403
1.196IleGln: 1.196 ± 0.293
3.65IleArg: 3.65 ± 0.526
3.209IleSer: 3.209 ± 0.466
3.272IleThr: 3.272 ± 0.461
2.769IleVal: 2.769 ± 0.517
0.566IleTrp: 0.566 ± 0.177
1.196IleTyr: 1.196 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
3.209LysAla: 3.209 ± 0.492
0.252LysCys: 0.252 ± 0.113
2.517LysAsp: 2.517 ± 0.494
2.077LysGlu: 2.077 ± 0.364
1.51LysPhe: 1.51 ± 0.307
2.265LysGly: 2.265 ± 0.356
0.881LysHis: 0.881 ± 0.307
2.769LysIle: 2.769 ± 0.528
2.139LysLys: 2.139 ± 0.509
3.335LysLeu: 3.335 ± 0.434
0.881LysMet: 0.881 ± 0.255
1.258LysAsn: 1.258 ± 0.243
2.706LysPro: 2.706 ± 0.427
1.447LysGln: 1.447 ± 0.263
3.398LysArg: 3.398 ± 0.456
2.328LysSer: 2.328 ± 0.414
2.454LysThr: 2.454 ± 0.423
3.335LysVal: 3.335 ± 0.453
0.692LysTrp: 0.692 ± 0.236
1.07LysTyr: 1.07 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
10.194LeuAla: 10.194 ± 0.873
0.252LeuCys: 0.252 ± 0.124
6.67LeuAsp: 6.67 ± 0.629
5.852LeuGlu: 5.852 ± 0.729
2.454LeuPhe: 2.454 ± 0.41
7.236LeuGly: 7.236 ± 0.808
1.51LeuHis: 1.51 ± 0.354
3.901LeuIle: 3.901 ± 0.497
3.398LeuLys: 3.398 ± 0.467
6.041LeuLeu: 6.041 ± 0.658
2.014LeuMet: 2.014 ± 0.342
2.643LeuAsn: 2.643 ± 0.334
5.223LeuPro: 5.223 ± 0.611
2.832LeuGln: 2.832 ± 0.459
6.292LeuArg: 6.292 ± 0.517
6.23LeuSer: 6.23 ± 0.703
5.978LeuThr: 5.978 ± 0.577
4.845LeuVal: 4.845 ± 0.641
1.321LeuTrp: 1.321 ± 0.401
2.517LeuTyr: 2.517 ± 0.469
0.0LeuXaa: 0.0 ± 0.0
Met
2.517MetAla: 2.517 ± 0.403
0.0MetCys: 0.0 ± 0.0
1.07MetAsp: 1.07 ± 0.246
1.573MetGlu: 1.573 ± 0.304
0.566MetPhe: 0.566 ± 0.188
1.447MetGly: 1.447 ± 0.246
0.315MetHis: 0.315 ± 0.128
0.755MetIle: 0.755 ± 0.21
1.007MetLys: 1.007 ± 0.215
1.133MetLeu: 1.133 ± 0.289
0.252MetMet: 0.252 ± 0.117
1.196MetAsn: 1.196 ± 0.255
1.258MetPro: 1.258 ± 0.318
0.629MetGln: 0.629 ± 0.195
1.196MetArg: 1.196 ± 0.281
2.391MetSer: 2.391 ± 0.396
2.139MetThr: 2.139 ± 0.334
1.196MetVal: 1.196 ± 0.284
0.315MetTrp: 0.315 ± 0.135
0.44MetTyr: 0.44 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
3.146AsnAla: 3.146 ± 0.473
0.189AsnCys: 0.189 ± 0.123
2.139AsnAsp: 2.139 ± 0.325
2.077AsnGlu: 2.077 ± 0.382
1.007AsnPhe: 1.007 ± 0.254
3.146AsnGly: 3.146 ± 0.442
0.629AsnHis: 0.629 ± 0.196
1.384AsnIle: 1.384 ± 0.306
0.692AsnLys: 0.692 ± 0.273
2.769AsnLeu: 2.769 ± 0.504
0.881AsnMet: 0.881 ± 0.213
0.881AsnAsn: 0.881 ± 0.227
2.706AsnPro: 2.706 ± 0.373
0.755AsnGln: 0.755 ± 0.218
1.258AsnArg: 1.258 ± 0.346
1.825AsnSer: 1.825 ± 0.435
2.077AsnThr: 2.077 ± 0.38
2.58AsnVal: 2.58 ± 0.382
0.755AsnTrp: 0.755 ± 0.178
1.07AsnTyr: 1.07 ± 0.27
0.0AsnXaa: 0.0 ± 0.0
Pro
4.845ProAla: 4.845 ± 0.533
0.378ProCys: 0.378 ± 0.146
4.405ProAsp: 4.405 ± 0.497
4.279ProGlu: 4.279 ± 0.494
2.139ProPhe: 2.139 ± 0.426
4.971ProGly: 4.971 ± 0.645
0.818ProHis: 0.818 ± 0.24
2.265ProIle: 2.265 ± 0.465
2.077ProLys: 2.077 ± 0.354
4.719ProLeu: 4.719 ± 0.628
1.321ProMet: 1.321 ± 0.326
1.699ProAsn: 1.699 ± 0.336
3.02ProPro: 3.02 ± 0.466
1.447ProGln: 1.447 ± 0.305
3.02ProArg: 3.02 ± 0.424
4.027ProSer: 4.027 ± 0.61
3.775ProThr: 3.775 ± 0.562
3.901ProVal: 3.901 ± 0.501
1.007ProTrp: 1.007 ± 0.31
1.762ProTyr: 1.762 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
3.524GlnAla: 3.524 ± 0.675
0.126GlnCys: 0.126 ± 0.095
1.321GlnAsp: 1.321 ± 0.285
1.321GlnGlu: 1.321 ± 0.282
1.196GlnPhe: 1.196 ± 0.265
2.706GlnGly: 2.706 ± 0.43
0.629GlnHis: 0.629 ± 0.176
2.454GlnIle: 2.454 ± 0.515
1.573GlnLys: 1.573 ± 0.312
3.65GlnLeu: 3.65 ± 0.474
0.755GlnMet: 0.755 ± 0.23
0.566GlnAsn: 0.566 ± 0.153
1.762GlnPro: 1.762 ± 0.299
1.762GlnGln: 1.762 ± 0.361
1.699GlnArg: 1.699 ± 0.486
1.573GlnSer: 1.573 ± 0.317
1.762GlnThr: 1.762 ± 0.326
2.643GlnVal: 2.643 ± 0.384
0.692GlnTrp: 0.692 ± 0.185
0.566GlnTyr: 0.566 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
5.789ArgAla: 5.789 ± 0.691
0.881ArgCys: 0.881 ± 0.268
2.895ArgAsp: 2.895 ± 0.465
4.719ArgGlu: 4.719 ± 0.62
1.951ArgPhe: 1.951 ± 0.392
5.16ArgGly: 5.16 ± 0.67
1.196ArgHis: 1.196 ± 0.294
2.895ArgIle: 2.895 ± 0.501
2.58ArgLys: 2.58 ± 0.471
6.167ArgLeu: 6.167 ± 0.806
2.014ArgMet: 2.014 ± 0.391
2.643ArgAsn: 2.643 ± 0.422
2.832ArgPro: 2.832 ± 0.467
1.762ArgGln: 1.762 ± 0.343
5.6ArgArg: 5.6 ± 0.711
3.901ArgSer: 3.901 ± 0.488
3.335ArgThr: 3.335 ± 0.526
5.223ArgVal: 5.223 ± 0.599
1.196ArgTrp: 1.196 ± 0.292
1.825ArgTyr: 1.825 ± 0.303
0.0ArgXaa: 0.0 ± 0.0
Ser
6.418SerAla: 6.418 ± 0.72
0.566SerCys: 0.566 ± 0.176
4.342SerAsp: 4.342 ± 0.504
3.964SerGlu: 3.964 ± 0.429
2.014SerPhe: 2.014 ± 0.374
5.789SerGly: 5.789 ± 0.67
1.51SerHis: 1.51 ± 0.288
2.202SerIle: 2.202 ± 0.368
2.202SerLys: 2.202 ± 0.308
4.908SerLeu: 4.908 ± 0.628
1.636SerMet: 1.636 ± 0.306
2.014SerAsn: 2.014 ± 0.379
3.146SerPro: 3.146 ± 0.487
1.951SerGln: 1.951 ± 0.287
3.335SerArg: 3.335 ± 0.494
3.65SerSer: 3.65 ± 0.624
3.461SerThr: 3.461 ± 0.535
3.398SerVal: 3.398 ± 0.443
1.447SerTrp: 1.447 ± 0.381
1.636SerTyr: 1.636 ± 0.389
0.0SerXaa: 0.0 ± 0.0
Thr
6.607ThrAla: 6.607 ± 0.781
0.189ThrCys: 0.189 ± 0.117
4.153ThrAsp: 4.153 ± 0.634
4.405ThrGlu: 4.405 ± 0.457
2.328ThrPhe: 2.328 ± 0.354
6.481ThrGly: 6.481 ± 0.584
0.944ThrHis: 0.944 ± 0.23
2.454ThrIle: 2.454 ± 0.54
2.391ThrLys: 2.391 ± 0.368
5.663ThrLeu: 5.663 ± 0.643
0.818ThrMet: 0.818 ± 0.176
2.014ThrAsn: 2.014 ± 0.369
4.027ThrPro: 4.027 ± 0.584
1.951ThrGln: 1.951 ± 0.372
3.65ThrArg: 3.65 ± 0.549
2.895ThrSer: 2.895 ± 0.411
4.279ThrThr: 4.279 ± 0.621
5.915ThrVal: 5.915 ± 0.696
1.133ThrTrp: 1.133 ± 0.257
2.014ThrTyr: 2.014 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
6.922ValAla: 6.922 ± 0.842
0.44ValCys: 0.44 ± 0.179
5.537ValAsp: 5.537 ± 0.637
4.908ValGlu: 4.908 ± 0.531
2.517ValPhe: 2.517 ± 0.317
4.656ValGly: 4.656 ± 0.636
1.321ValHis: 1.321 ± 0.271
3.65ValIle: 3.65 ± 0.518
3.524ValLys: 3.524 ± 0.518
5.412ValLeu: 5.412 ± 0.584
1.321ValMet: 1.321 ± 0.341
2.517ValAsn: 2.517 ± 0.348
4.216ValPro: 4.216 ± 0.478
2.265ValGln: 2.265 ± 0.419
4.908ValArg: 4.908 ± 0.703
4.782ValSer: 4.782 ± 0.545
5.663ValThr: 5.663 ± 0.594
4.719ValVal: 4.719 ± 0.74
1.258ValTrp: 1.258 ± 0.303
2.517ValTyr: 2.517 ± 0.456
0.0ValXaa: 0.0 ± 0.0
Trp
1.51TrpAla: 1.51 ± 0.323
0.189TrpCys: 0.189 ± 0.103
1.573TrpAsp: 1.573 ± 0.29
0.944TrpGlu: 0.944 ± 0.229
1.07TrpPhe: 1.07 ± 0.319
1.636TrpGly: 1.636 ± 0.305
0.503TrpHis: 0.503 ± 0.168
1.133TrpIle: 1.133 ± 0.267
0.566TrpLys: 0.566 ± 0.216
1.951TrpLeu: 1.951 ± 0.404
0.378TrpMet: 0.378 ± 0.182
0.566TrpAsn: 0.566 ± 0.187
0.755TrpPro: 0.755 ± 0.241
1.007TrpGln: 1.007 ± 0.236
1.258TrpArg: 1.258 ± 0.25
1.133TrpSer: 1.133 ± 0.301
1.51TrpThr: 1.51 ± 0.397
1.888TrpVal: 1.888 ± 0.299
0.692TrpTrp: 0.692 ± 0.252
0.315TrpTyr: 0.315 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.895TyrAla: 2.895 ± 0.431
0.189TyrCys: 0.189 ± 0.153
1.196TyrAsp: 1.196 ± 0.251
2.139TyrGlu: 2.139 ± 0.355
0.503TyrPhe: 0.503 ± 0.177
2.832TyrGly: 2.832 ± 0.48
0.629TyrHis: 0.629 ± 0.19
1.447TyrIle: 1.447 ± 0.364
1.07TyrLys: 1.07 ± 0.221
2.706TyrLeu: 2.706 ± 0.418
0.503TyrMet: 0.503 ± 0.166
1.321TyrAsn: 1.321 ± 0.375
1.258TyrPro: 1.258 ± 0.315
1.133TyrGln: 1.133 ± 0.264
2.643TyrArg: 2.643 ± 0.403
1.762TyrSer: 1.762 ± 0.297
2.202TyrThr: 2.202 ± 0.441
1.762TyrVal: 1.762 ± 0.301
0.315TyrTrp: 0.315 ± 0.134
0.503TyrTyr: 0.503 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (15893 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski