Salmonella phage ZCSE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Loughboroughvirus; Salmonella virus ZCSE2

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DW68|A0A4D6DW68_9CAUD Uncharacterized protein OS=Salmonella phage ZCSE2 OX=2562175 PE=4 SV=1
MM1 pKa = 7.39TPFIINQHH9 pKa = 4.41NCYY12 pKa = 10.08FYY14 pKa = 10.51LTNEE18 pKa = 4.46LVEE21 pKa = 4.11MMQGQEE27 pKa = 4.09LTNCRR32 pKa = 11.84CFDD35 pKa = 3.78NFDD38 pKa = 3.55DD39 pKa = 5.6FYY41 pKa = 11.59AAVHH45 pKa = 6.54EE46 pKa = 4.62IYY48 pKa = 10.13PAIEE52 pKa = 4.29SVEE55 pKa = 4.1EE56 pKa = 4.31LYY58 pKa = 11.07GSYY61 pKa = 10.32IEE63 pKa = 5.94LYY65 pKa = 10.88YY66 pKa = 10.95DD67 pKa = 4.54DD68 pKa = 5.7EE69 pKa = 5.37LDD71 pKa = 3.68KK72 pKa = 11.52AVVEE76 pKa = 4.49HH77 pKa = 6.72SFLNAVFDD85 pKa = 4.05IEE87 pKa = 4.4TLAEE91 pKa = 4.96DD92 pKa = 3.69ISNYY96 pKa = 8.81TADD99 pKa = 4.9DD100 pKa = 3.7EE101 pKa = 4.64

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DVU9|A0A4D6DVU9_9CAUD Tail protein I OS=Salmonella phage ZCSE2 OX=2562175 PE=4 SV=1
MM1 pKa = 7.35FRR3 pKa = 11.84IRR5 pKa = 11.84LEE7 pKa = 3.92RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.51KK11 pKa = 11.14QKK13 pKa = 11.29FEE15 pKa = 3.54FTYY18 pKa = 10.71RR19 pKa = 11.84AFKK22 pKa = 10.65SFNGQGVTVGVHH34 pKa = 5.66PDD36 pKa = 2.78KK37 pKa = 11.03NRR39 pKa = 11.84RR40 pKa = 11.84SDD42 pKa = 3.49PKK44 pKa = 10.94DD45 pKa = 3.69GIGNAEE51 pKa = 4.07LAMVHH56 pKa = 6.8EE57 pKa = 4.9YY58 pKa = 11.27GLNNVPEE65 pKa = 4.24RR66 pKa = 11.84SFLRR70 pKa = 11.84SAVGGRR76 pKa = 11.84GKK78 pKa = 10.48GRR80 pKa = 11.84KK81 pKa = 9.1AINRR85 pKa = 11.84AFKK88 pKa = 10.39EE89 pKa = 4.11GVPAVLRR96 pKa = 11.84GDD98 pKa = 3.55MTAEE102 pKa = 3.85QLNDD106 pKa = 3.46QIGQMLVEE114 pKa = 4.44AVRR117 pKa = 11.84ARR119 pKa = 11.84MRR121 pKa = 11.84EE122 pKa = 4.2NIPPPLKK129 pKa = 10.64PEE131 pKa = 4.04TEE133 pKa = 4.12RR134 pKa = 11.84KK135 pKa = 7.95KK136 pKa = 9.33TGPGTLRR143 pKa = 11.84EE144 pKa = 4.09SMEE147 pKa = 4.32LYY149 pKa = 10.49EE150 pKa = 4.63SIGYY154 pKa = 9.32KK155 pKa = 9.94VGRR158 pKa = 11.84NWRR161 pKa = 11.84LVNNAA166 pKa = 3.94

Molecular weight: 18.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16487

30

980

211.4

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.352 ± 0.314

1.31 ± 0.12

6.472 ± 0.184

6.253 ± 0.305

3.579 ± 0.162

6.902 ± 0.248

1.735 ± 0.14

5.774 ± 0.184

6.29 ± 0.291

8.018 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.155

5.059 ± 0.204

4.41 ± 0.195

3.858 ± 0.212

4.682 ± 0.273

5.526 ± 0.238

6.581 ± 0.473

7.163 ± 0.341

1.638 ± 0.136

3.536 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski