Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4500 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9MHB1|A9MHB1_SALAR Uncharacterized protein OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=SARI_03465 PE=4 SV=1
MM1 pKa = 7.62 EE2 pKa = 5.26 NLLNRR7 pKa = 11.84 LYY9 pKa = 10.96 DD10 pKa = 3.93 ALGLDD15 pKa = 3.81 APEE18 pKa = 5.33 DD19 pKa = 4.06 EE20 pKa = 5.17 PLLIIDD26 pKa = 5.75 DD27 pKa = 5.1 EE28 pKa = 4.44 IQVYY32 pKa = 10.17 FNEE35 pKa = 4.24 SDD37 pKa = 4.35 LMLEE41 pKa = 4.21 MCCPFMPFPDD51 pKa = 4.75 DD52 pKa = 5.21 LLTLQHH58 pKa = 6.94 FLRR61 pKa = 11.84 LNYY64 pKa = 8.7 TSPVTIGADD73 pKa = 3.4 ADD75 pKa = 3.79 NTALVALYY83 pKa = 9.8 RR84 pKa = 11.84 LPQTSTEE91 pKa = 4.09 EE92 pKa = 3.74 EE93 pKa = 4.21 ALTGFEE99 pKa = 4.67 LFISSVKK106 pKa = 10.51 KK107 pKa = 10.61 FIEE110 pKa = 4.91 HH111 pKa = 4.77 YY112 pKa = 9.13 TT113 pKa = 3.47
Molecular weight: 12.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.643
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.745
Sillero 3.948
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>sp|A9MJU2|RECF_SALAR DNA replication and repair protein RecF OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=recF PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4500
0
4500
1329514
30
7354
295.4
32.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.54 ± 0.044
1.146 ± 0.018
5.221 ± 0.043
5.591 ± 0.044
3.942 ± 0.026
7.271 ± 0.039
2.304 ± 0.018
5.979 ± 0.036
4.365 ± 0.036
10.676 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.023
3.935 ± 0.032
4.44 ± 0.027
4.386 ± 0.034
5.697 ± 0.039
5.832 ± 0.032
5.526 ± 0.056
6.933 ± 0.039
1.518 ± 0.017
2.916 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here