Clostridium sp. CAG:470

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7FIJ4|R7FIJ4_9CLOT Phosphoglucosamine mutase OS=Clostridium sp. CAG:470 OX=1262812 GN=glmM PE=3 SV=1
MM1 pKa = 7.8KK2 pKa = 10.17YY3 pKa = 10.14KK4 pKa = 9.8CTACGYY10 pKa = 10.06IYY12 pKa = 10.17DD13 pKa = 4.81DD14 pKa = 3.54NVEE17 pKa = 4.31TVKK20 pKa = 11.05FEE22 pKa = 4.74DD23 pKa = 5.69LPDD26 pKa = 3.8DD27 pKa = 4.32WVCPLCGLGKK37 pKa = 10.44EE38 pKa = 4.15FFEE41 pKa = 5.71KK42 pKa = 10.12IEE44 pKa = 4.03EE45 pKa = 4.14

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7FJJ2|R7FJJ2_9CLOT Sporulation protein YqfC OS=Clostridium sp. CAG:470 OX=1262812 GN=BN670_01063 PE=4 SV=1
MM1 pKa = 7.6NYY3 pKa = 9.76IFLDD7 pKa = 3.06IDD9 pKa = 3.66GVLNNKK15 pKa = 8.34KK16 pKa = 9.9HH17 pKa = 5.91YY18 pKa = 10.14SKK20 pKa = 10.27QHH22 pKa = 5.09KK23 pKa = 9.9KK24 pKa = 9.84YY25 pKa = 10.5GGRR28 pKa = 11.84FCCEE32 pKa = 3.23NMPFNPRR39 pKa = 11.84SILNLRR45 pKa = 11.84KK46 pKa = 9.83IIDD49 pKa = 3.57KK50 pKa = 10.07TNSKK54 pKa = 10.31VVISSSWRR62 pKa = 11.84RR63 pKa = 11.84TKK65 pKa = 11.09NGMIVLKK72 pKa = 10.7ARR74 pKa = 11.84LIEE77 pKa = 4.0YY78 pKa = 9.48GIKK81 pKa = 9.67IYY83 pKa = 10.91SVTPFISRR91 pKa = 11.84RR92 pKa = 3.59

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1535

0

1535

446944

29

6242

291.2

33.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.125 ± 0.059

1.082 ± 0.028

5.551 ± 0.046

8.157 ± 0.089

3.836 ± 0.063

5.837 ± 0.071

1.153 ± 0.024

9.966 ± 0.076

10.317 ± 0.071

8.056 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.036

7.214 ± 0.083

2.463 ± 0.038

2.943 ± 0.035

3.063 ± 0.052

5.664 ± 0.063

5.952 ± 0.117

6.023 ± 0.049

0.667 ± 0.023

4.498 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski