Peptococcaceae bacterium SCADC1_2_3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; unclassified Peptococcaceae

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086YT85|A0A086YT85_9FIRM Uncharacterized protein OS=Peptococcaceae bacterium SCADC1_2_3 OX=1487582 GN=DK28_0203360 PE=4 SV=1
MM1 pKa = 7.46GKK3 pKa = 9.73KK4 pKa = 9.84KK5 pKa = 10.4KK6 pKa = 7.88EE7 pKa = 4.07QEE9 pKa = 3.91WPEE12 pKa = 3.91EE13 pKa = 3.97PEE15 pKa = 4.09EE16 pKa = 4.03FVQAMLGIGEE26 pKa = 4.17EE27 pKa = 4.08TYY29 pKa = 11.35YY30 pKa = 10.99NYY32 pKa = 10.51RR33 pKa = 11.84CSQCNYY39 pKa = 9.43EE40 pKa = 4.15EE41 pKa = 4.06QVPDD45 pKa = 4.01FVVDD49 pKa = 3.74EE50 pKa = 4.35LAAFDD55 pKa = 4.33EE56 pKa = 4.92LPPGVMPEE64 pKa = 4.57LEE66 pKa = 4.69CGNCGGTLKK75 pKa = 10.95CVDD78 pKa = 3.46

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085L7P1|A0A085L7P1_9FIRM Pyridinium-3 5-bisthiocarboxylic acid mononucleotide nickel insertion protein OS=Peptococcaceae bacterium SCADC1_2_3 OX=1487582 GN=larC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPKK8 pKa = 7.39VRR10 pKa = 11.84KK11 pKa = 9.46RR12 pKa = 11.84KK13 pKa = 8.84RR14 pKa = 11.84CHH16 pKa = 6.09GFLKK20 pKa = 10.43RR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84SIIKK32 pKa = 9.92RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.51KK38 pKa = 9.96RR39 pKa = 11.84KK40 pKa = 8.84EE41 pKa = 3.7LSAA44 pKa = 4.86

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2187

0

2187

565976

37

1387

258.8

28.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.001 ± 0.056

1.162 ± 0.02

4.402 ± 0.043

7.315 ± 0.054

4.181 ± 0.046

7.199 ± 0.046

1.743 ± 0.023

7.314 ± 0.05

6.965 ± 0.052

10.749 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.022

4.058 ± 0.039

4.311 ± 0.03

3.724 ± 0.037

5.146 ± 0.043

4.899 ± 0.033

5.039 ± 0.033

7.226 ± 0.05

1.043 ± 0.023

3.352 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski