Amino acid dipepetide frequency for Clostridium phage phiCT453A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.713AlaAla: 1.713 ± 0.342
0.389AlaCys: 0.389 ± 0.161
2.492AlaAsp: 2.492 ± 0.424
3.738AlaGlu: 3.738 ± 0.693
2.025AlaPhe: 2.025 ± 0.5
2.258AlaGly: 2.258 ± 0.384
0.545AlaHis: 0.545 ± 0.191
4.984AlaIle: 4.984 ± 0.584
4.75AlaLys: 4.75 ± 0.713
5.529AlaLeu: 5.529 ± 0.816
0.857AlaMet: 0.857 ± 0.294
3.271AlaAsn: 3.271 ± 0.524
1.09AlaPro: 1.09 ± 0.263
1.402AlaGln: 1.402 ± 0.324
2.648AlaArg: 2.648 ± 0.496
2.804AlaSer: 2.804 ± 0.586
3.271AlaThr: 3.271 ± 0.542
2.414AlaVal: 2.414 ± 0.497
0.623AlaTrp: 0.623 ± 0.205
1.947AlaTyr: 1.947 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.312CysAla: 0.312 ± 0.141
0.0CysCys: 0.0 ± 0.0
0.935CysAsp: 0.935 ± 0.242
0.312CysGlu: 0.312 ± 0.143
0.467CysPhe: 0.467 ± 0.171
0.234CysGly: 0.234 ± 0.137
0.078CysHis: 0.078 ± 0.066
0.467CysIle: 0.467 ± 0.158
1.48CysLys: 1.48 ± 0.378
0.389CysLeu: 0.389 ± 0.139
0.234CysMet: 0.234 ± 0.157
1.246CysAsn: 1.246 ± 0.311
0.312CysPro: 0.312 ± 0.149
0.234CysGln: 0.234 ± 0.123
0.701CysArg: 0.701 ± 0.241
0.312CysSer: 0.312 ± 0.139
0.389CysThr: 0.389 ± 0.164
0.234CysVal: 0.234 ± 0.146
0.0CysTrp: 0.0 ± 0.0
0.467CysTyr: 0.467 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
1.869AspAla: 1.869 ± 0.254
0.545AspCys: 0.545 ± 0.189
4.984AspAsp: 4.984 ± 0.727
4.361AspGlu: 4.361 ± 0.538
3.271AspPhe: 3.271 ± 0.62
3.504AspGly: 3.504 ± 0.419
0.467AspHis: 0.467 ± 0.162
6.386AspIle: 6.386 ± 0.658
6.464AspLys: 6.464 ± 0.783
4.906AspLeu: 4.906 ± 0.669
2.025AspMet: 2.025 ± 0.369
3.66AspAsn: 3.66 ± 0.489
1.635AspPro: 1.635 ± 0.386
1.324AspGln: 1.324 ± 0.255
1.791AspArg: 1.791 ± 0.402
2.959AspSer: 2.959 ± 0.512
4.05AspThr: 4.05 ± 0.536
3.894AspVal: 3.894 ± 0.499
0.623AspTrp: 0.623 ± 0.191
2.804AspTyr: 2.804 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
3.504GluAla: 3.504 ± 0.532
0.545GluCys: 0.545 ± 0.287
4.439GluAsp: 4.439 ± 0.599
8.177GluGlu: 8.177 ± 1.055
3.816GluPhe: 3.816 ± 0.609
4.828GluGly: 4.828 ± 0.578
0.857GluHis: 0.857 ± 0.24
8.566GluIle: 8.566 ± 1.238
8.099GluLys: 8.099 ± 1.008
7.476GluLeu: 7.476 ± 0.793
1.791GluMet: 1.791 ± 0.367
6.464GluAsn: 6.464 ± 0.788
1.635GluPro: 1.635 ± 0.337
2.959GluGln: 2.959 ± 0.434
3.193GluArg: 3.193 ± 0.63
2.726GluSer: 2.726 ± 0.42
4.439GluThr: 4.439 ± 0.539
6.775GluVal: 6.775 ± 1.03
0.857GluTrp: 0.857 ± 0.194
3.271GluTyr: 3.271 ± 0.515
0.0GluXaa: 0.0 ± 0.0
Phe
2.103PheAla: 2.103 ± 0.376
0.701PheCys: 0.701 ± 0.184
3.037PheAsp: 3.037 ± 0.535
3.037PheGlu: 3.037 ± 0.483
1.402PhePhe: 1.402 ± 0.369
3.816PheGly: 3.816 ± 0.848
0.389PheHis: 0.389 ± 0.202
3.894PheIle: 3.894 ± 0.593
4.439PheLys: 4.439 ± 0.721
2.881PheLeu: 2.881 ± 0.432
1.246PheMet: 1.246 ± 0.327
3.193PheAsn: 3.193 ± 0.558
1.246PhePro: 1.246 ± 0.311
0.701PheGln: 0.701 ± 0.221
1.09PheArg: 1.09 ± 0.291
2.881PheSer: 2.881 ± 0.429
1.869PheThr: 1.869 ± 0.318
1.48PheVal: 1.48 ± 0.309
0.467PheTrp: 0.467 ± 0.194
1.168PheTyr: 1.168 ± 0.261
0.0PheXaa: 0.0 ± 0.0
Gly
4.05GlyAla: 4.05 ± 0.737
0.467GlyCys: 0.467 ± 0.285
3.738GlyAsp: 3.738 ± 0.452
5.062GlyGlu: 5.062 ± 0.64
2.726GlyPhe: 2.726 ± 0.493
3.193GlyGly: 3.193 ± 0.578
0.935GlyHis: 0.935 ± 0.331
6.386GlyIle: 6.386 ± 0.769
5.607GlyLys: 5.607 ± 0.873
3.972GlyLeu: 3.972 ± 0.806
1.246GlyMet: 1.246 ± 0.301
4.283GlyAsn: 4.283 ± 0.793
0.545GlyPro: 0.545 ± 0.156
1.869GlyGln: 1.869 ± 0.284
2.57GlyArg: 2.57 ± 0.488
2.726GlySer: 2.726 ± 0.406
2.726GlyThr: 2.726 ± 0.557
4.127GlyVal: 4.127 ± 0.596
0.389GlyTrp: 0.389 ± 0.21
2.726GlyTyr: 2.726 ± 0.568
0.0GlyXaa: 0.0 ± 0.0
His
0.545HisAla: 0.545 ± 0.223
0.078HisCys: 0.078 ± 0.07
0.545HisAsp: 0.545 ± 0.253
1.168HisGlu: 1.168 ± 0.289
0.623HisPhe: 0.623 ± 0.219
0.935HisGly: 0.935 ± 0.277
0.234HisHis: 0.234 ± 0.13
0.545HisIle: 0.545 ± 0.185
1.012HisLys: 1.012 ± 0.298
1.48HisLeu: 1.48 ± 0.3
0.234HisMet: 0.234 ± 0.126
0.467HisAsn: 0.467 ± 0.221
0.545HisPro: 0.545 ± 0.163
0.312HisGln: 0.312 ± 0.157
0.467HisArg: 0.467 ± 0.164
0.312HisSer: 0.312 ± 0.148
0.857HisThr: 0.857 ± 0.247
0.312HisVal: 0.312 ± 0.155
0.234HisTrp: 0.234 ± 0.116
0.156HisTyr: 0.156 ± 0.116
0.0HisXaa: 0.0 ± 0.0
Ile
4.828IleAla: 4.828 ± 0.503
1.012IleCys: 1.012 ± 0.324
5.218IleAsp: 5.218 ± 0.515
8.411IleGlu: 8.411 ± 0.682
3.271IlePhe: 3.271 ± 0.564
4.906IleGly: 4.906 ± 0.606
1.402IleHis: 1.402 ± 0.348
7.242IleIle: 7.242 ± 0.774
10.903IleLys: 10.903 ± 1.083
6.386IleLeu: 6.386 ± 0.685
1.558IleMet: 1.558 ± 0.35
6.308IleAsn: 6.308 ± 0.801
2.648IlePro: 2.648 ± 0.505
2.414IleGln: 2.414 ± 0.455
3.349IleArg: 3.349 ± 0.566
6.23IleSer: 6.23 ± 0.738
4.673IleThr: 4.673 ± 0.602
4.361IleVal: 4.361 ± 0.602
0.935IleTrp: 0.935 ± 0.3
3.66IleTyr: 3.66 ± 0.58
0.0IleXaa: 0.0 ± 0.0
Lys
5.763LysAla: 5.763 ± 0.755
0.623LysCys: 0.623 ± 0.193
7.009LysAsp: 7.009 ± 0.803
10.903LysGlu: 10.903 ± 1.51
3.193LysPhe: 3.193 ± 0.43
5.685LysGly: 5.685 ± 0.565
1.324LysHis: 1.324 ± 0.305
8.177LysIle: 8.177 ± 0.914
9.657LysLys: 9.657 ± 1.28
8.021LysLeu: 8.021 ± 0.969
2.804LysMet: 2.804 ± 0.526
7.71LysAsn: 7.71 ± 1.03
2.336LysPro: 2.336 ± 0.406
4.517LysGln: 4.517 ± 0.577
3.66LysArg: 3.66 ± 0.571
4.517LysSer: 4.517 ± 0.505
6.308LysThr: 6.308 ± 0.615
6.308LysVal: 6.308 ± 0.804
0.935LysTrp: 0.935 ± 0.292
4.205LysTyr: 4.205 ± 0.47
0.0LysXaa: 0.0 ± 0.0
Leu
3.504LeuAla: 3.504 ± 0.664
0.701LeuCys: 0.701 ± 0.212
4.906LeuAsp: 4.906 ± 0.695
7.242LeuGlu: 7.242 ± 0.926
3.115LeuPhe: 3.115 ± 0.519
5.996LeuGly: 5.996 ± 0.754
0.623LeuHis: 0.623 ± 0.25
6.23LeuIle: 6.23 ± 0.679
10.747LeuLys: 10.747 ± 1.04
5.763LeuLeu: 5.763 ± 0.696
1.947LeuMet: 1.947 ± 0.366
6.464LeuAsn: 6.464 ± 0.719
2.025LeuPro: 2.025 ± 0.67
2.726LeuGln: 2.726 ± 0.39
3.271LeuArg: 3.271 ± 0.529
4.906LeuSer: 4.906 ± 0.542
4.439LeuThr: 4.439 ± 0.446
4.127LeuVal: 4.127 ± 0.44
1.012LeuTrp: 1.012 ± 0.286
2.57LeuTyr: 2.57 ± 0.422
0.0LeuXaa: 0.0 ± 0.0
Met
1.324MetAla: 1.324 ± 0.341
0.156MetCys: 0.156 ± 0.101
1.947MetAsp: 1.947 ± 0.374
2.57MetGlu: 2.57 ± 0.484
1.012MetPhe: 1.012 ± 0.251
1.713MetGly: 1.713 ± 0.382
0.312MetHis: 0.312 ± 0.154
1.48MetIle: 1.48 ± 0.335
1.947MetLys: 1.947 ± 0.431
2.258MetLeu: 2.258 ± 0.403
0.389MetMet: 0.389 ± 0.157
1.402MetAsn: 1.402 ± 0.268
1.012MetPro: 1.012 ± 0.249
0.779MetGln: 0.779 ± 0.246
0.234MetArg: 0.234 ± 0.118
1.635MetSer: 1.635 ± 0.427
1.168MetThr: 1.168 ± 0.221
0.779MetVal: 0.779 ± 0.242
0.0MetTrp: 0.0 ± 0.0
1.324MetTyr: 1.324 ± 0.335
0.0MetXaa: 0.0 ± 0.0
Asn
3.271AsnAla: 3.271 ± 0.659
1.168AsnCys: 1.168 ± 0.327
2.57AsnAsp: 2.57 ± 0.394
4.673AsnGlu: 4.673 ± 0.697
2.57AsnPhe: 2.57 ± 0.454
4.439AsnGly: 4.439 ± 0.509
0.779AsnHis: 0.779 ± 0.225
6.464AsnIle: 6.464 ± 0.719
9.034AsnLys: 9.034 ± 1.039
5.841AsnLeu: 5.841 ± 0.785
2.181AsnMet: 2.181 ± 0.531
6.697AsnAsn: 6.697 ± 0.958
2.258AsnPro: 2.258 ± 0.488
1.869AsnGln: 1.869 ± 0.364
3.66AsnArg: 3.66 ± 0.436
3.972AsnSer: 3.972 ± 0.771
5.607AsnThr: 5.607 ± 1.127
3.504AsnVal: 3.504 ± 0.635
0.701AsnTrp: 0.701 ± 0.235
2.336AsnTyr: 2.336 ± 0.37
0.0AsnXaa: 0.0 ± 0.0
Pro
0.779ProAla: 0.779 ± 0.206
0.467ProCys: 0.467 ± 0.219
2.025ProAsp: 2.025 ± 0.445
2.103ProGlu: 2.103 ± 0.472
0.623ProPhe: 0.623 ± 0.203
1.246ProGly: 1.246 ± 0.315
0.234ProHis: 0.234 ± 0.127
2.726ProIle: 2.726 ± 0.438
2.648ProLys: 2.648 ± 0.484
2.258ProLeu: 2.258 ± 0.408
0.389ProMet: 0.389 ± 0.168
1.713ProAsn: 1.713 ± 0.347
0.779ProPro: 0.779 ± 0.189
0.857ProGln: 0.857 ± 0.205
0.701ProArg: 0.701 ± 0.199
1.324ProSer: 1.324 ± 0.283
1.558ProThr: 1.558 ± 0.329
1.635ProVal: 1.635 ± 0.328
0.701ProTrp: 0.701 ± 0.24
0.857ProTyr: 0.857 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
1.869GlnAla: 1.869 ± 0.321
0.389GlnCys: 0.389 ± 0.183
1.947GlnAsp: 1.947 ± 0.402
2.648GlnGlu: 2.648 ± 0.439
1.012GlnPhe: 1.012 ± 0.214
2.258GlnGly: 2.258 ± 0.348
0.312GlnHis: 0.312 ± 0.127
2.881GlnIle: 2.881 ± 0.583
2.258GlnLys: 2.258 ± 0.379
2.57GlnLeu: 2.57 ± 0.41
0.545GlnMet: 0.545 ± 0.184
2.103GlnAsn: 2.103 ± 0.33
0.857GlnPro: 0.857 ± 0.201
0.935GlnGln: 0.935 ± 0.246
1.168GlnArg: 1.168 ± 0.299
1.324GlnSer: 1.324 ± 0.309
1.48GlnThr: 1.48 ± 0.326
1.558GlnVal: 1.558 ± 0.342
0.467GlnTrp: 0.467 ± 0.254
1.09GlnTyr: 1.09 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
1.947ArgAla: 1.947 ± 0.337
0.312ArgCys: 0.312 ± 0.12
1.869ArgAsp: 1.869 ± 0.322
2.258ArgGlu: 2.258 ± 0.437
1.558ArgPhe: 1.558 ± 0.342
1.48ArgGly: 1.48 ± 0.297
0.156ArgHis: 0.156 ± 0.118
3.504ArgIle: 3.504 ± 0.55
3.66ArgLys: 3.66 ± 0.523
4.283ArgLeu: 4.283 ± 0.725
1.246ArgMet: 1.246 ± 0.328
3.115ArgAsn: 3.115 ± 0.519
0.779ArgPro: 0.779 ± 0.193
1.09ArgGln: 1.09 ± 0.3
1.246ArgArg: 1.246 ± 0.372
2.103ArgSer: 2.103 ± 0.343
2.648ArgThr: 2.648 ± 0.468
1.869ArgVal: 1.869 ± 0.356
0.545ArgTrp: 0.545 ± 0.23
2.181ArgTyr: 2.181 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
3.193SerAla: 3.193 ± 0.698
0.156SerCys: 0.156 ± 0.089
2.258SerAsp: 2.258 ± 0.489
3.894SerGlu: 3.894 ± 0.553
2.492SerPhe: 2.492 ± 0.48
2.726SerGly: 2.726 ± 0.431
0.312SerHis: 0.312 ± 0.142
5.14SerIle: 5.14 ± 0.673
6.074SerLys: 6.074 ± 0.726
3.193SerLeu: 3.193 ± 0.447
1.635SerMet: 1.635 ± 0.355
5.14SerAsn: 5.14 ± 0.768
0.779SerPro: 0.779 ± 0.242
1.48SerGln: 1.48 ± 0.384
2.726SerArg: 2.726 ± 0.791
3.115SerSer: 3.115 ± 0.623
3.66SerThr: 3.66 ± 0.562
2.804SerVal: 2.804 ± 0.492
0.623SerTrp: 0.623 ± 0.246
2.103SerTyr: 2.103 ± 0.466
0.0SerXaa: 0.0 ± 0.0
Thr
3.193ThrAla: 3.193 ± 0.503
0.467ThrCys: 0.467 ± 0.17
3.972ThrAsp: 3.972 ± 0.532
3.582ThrGlu: 3.582 ± 0.47
3.193ThrPhe: 3.193 ± 0.688
3.504ThrGly: 3.504 ± 0.648
0.935ThrHis: 0.935 ± 0.236
4.673ThrIle: 4.673 ± 0.703
5.373ThrLys: 5.373 ± 0.509
6.697ThrLeu: 6.697 ± 0.716
1.012ThrMet: 1.012 ± 0.246
3.271ThrAsn: 3.271 ± 0.425
1.869ThrPro: 1.869 ± 0.334
1.168ThrGln: 1.168 ± 0.306
1.947ThrArg: 1.947 ± 0.365
2.726ThrSer: 2.726 ± 0.58
3.582ThrThr: 3.582 ± 0.498
3.349ThrVal: 3.349 ± 0.548
0.857ThrTrp: 0.857 ± 0.246
3.037ThrTyr: 3.037 ± 0.494
0.0ThrXaa: 0.0 ± 0.0
Val
2.025ValAla: 2.025 ± 0.402
0.156ValCys: 0.156 ± 0.109
4.127ValAsp: 4.127 ± 0.589
5.841ValGlu: 5.841 ± 0.893
2.103ValPhe: 2.103 ± 0.475
3.427ValGly: 3.427 ± 0.533
0.467ValHis: 0.467 ± 0.184
6.464ValIle: 6.464 ± 0.864
4.361ValLys: 4.361 ± 0.674
4.127ValLeu: 4.127 ± 0.534
1.246ValMet: 1.246 ± 0.33
3.504ValAsn: 3.504 ± 0.449
2.181ValPro: 2.181 ± 0.466
2.103ValGln: 2.103 ± 0.388
1.635ValArg: 1.635 ± 0.262
3.037ValSer: 3.037 ± 0.555
2.804ValThr: 2.804 ± 0.497
3.504ValVal: 3.504 ± 0.523
0.389ValTrp: 0.389 ± 0.152
2.336ValTyr: 2.336 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
0.935TrpAla: 0.935 ± 0.283
0.078TrpCys: 0.078 ± 0.066
0.623TrpAsp: 0.623 ± 0.266
0.935TrpGlu: 0.935 ± 0.258
0.545TrpPhe: 0.545 ± 0.18
0.701TrpGly: 0.701 ± 0.223
0.234TrpHis: 0.234 ± 0.136
0.545TrpIle: 0.545 ± 0.22
0.935TrpLys: 0.935 ± 0.226
1.012TrpLeu: 1.012 ± 0.292
0.078TrpMet: 0.078 ± 0.086
0.857TrpAsn: 0.857 ± 0.205
0.156TrpPro: 0.156 ± 0.146
0.389TrpGln: 0.389 ± 0.138
0.389TrpArg: 0.389 ± 0.184
0.935TrpSer: 0.935 ± 0.242
0.312TrpThr: 0.312 ± 0.163
0.857TrpVal: 0.857 ± 0.238
0.078TrpTrp: 0.078 ± 0.081
0.467TrpTyr: 0.467 ± 0.239
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.025TyrAla: 2.025 ± 0.408
0.467TyrCys: 0.467 ± 0.191
2.881TyrAsp: 2.881 ± 0.387
3.504TyrGlu: 3.504 ± 0.631
2.025TyrPhe: 2.025 ± 0.432
2.492TyrGly: 2.492 ± 0.436
0.467TyrHis: 0.467 ± 0.163
2.959TyrIle: 2.959 ± 0.473
4.205TyrLys: 4.205 ± 0.6
3.271TyrLeu: 3.271 ± 0.491
0.701TyrMet: 0.701 ± 0.241
2.726TyrAsn: 2.726 ± 0.581
0.935TyrPro: 0.935 ± 0.304
0.545TyrGln: 0.545 ± 0.186
1.402TyrArg: 1.402 ± 0.441
2.959TyrSer: 2.959 ± 0.599
2.57TyrThr: 2.57 ± 0.59
1.947TyrVal: 1.947 ± 0.489
0.623TyrTrp: 0.623 ± 0.206
1.713TyrTyr: 1.713 ± 0.374
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12842 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski