Lactobacillus equigenerosi DSM 18793 = JCM 14505
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1490 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1UH54|A0A0R1UH54_9LACO N-acetyltransferase domain-containing protein OS=Lactobacillus equigenerosi DSM 18793 = JCM 14505 OX=1423742 GN=FC21_GL000215 PE=4 SV=1
MM1 pKa = 7.48 LSIGSIVYY9 pKa = 10.61 LEE11 pKa = 4.99 GGDD14 pKa = 3.65 QKK16 pKa = 11.82 LMIIGQGQIMEE27 pKa = 4.21 QNKK30 pKa = 7.43 QQVYY34 pKa = 9.44 FDD36 pKa = 4.37 YY37 pKa = 11.0 VACQYY42 pKa = 10.95 PSGIDD47 pKa = 3.53 PEE49 pKa = 4.22 EE50 pKa = 3.67 VYY52 pKa = 10.97 YY53 pKa = 10.66 FNKK56 pKa = 10.14 EE57 pKa = 3.97 DD58 pKa = 3.36 IAEE61 pKa = 4.31 VVFDD65 pKa = 4.33 GYY67 pKa = 11.05 RR68 pKa = 11.84 DD69 pKa = 3.64 EE70 pKa = 5.22 EE71 pKa = 4.71 SYY73 pKa = 11.24 KK74 pKa = 10.25 VDD76 pKa = 3.14 QLYY79 pKa = 9.91 QEE81 pKa = 4.77 WIEE84 pKa = 4.22 KK85 pKa = 9.53 TDD87 pKa = 3.72 LPKK90 pKa = 11.23 GEE92 pKa = 4.29 TSLL95 pKa = 4.47
Molecular weight: 11.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.844
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.757
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.77
Grimsley 3.668
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.177
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.05
Patrickios 0.604
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A0R1UN19|A0A0R1UN19_9LACO HAD superfamily (Subfamily IIIA) phosphatase OS=Lactobacillus equigenerosi DSM 18793 = JCM 14505 OX=1423742 GN=FC21_GL000376 PE=4 SV=1
MM1 pKa = 7.1 NQQLHH6 pKa = 6.04 PQAKK10 pKa = 8.75 VQQHH14 pKa = 5.5 PQANQQLRR22 pKa = 11.84 QQAKK26 pKa = 6.94 VQRR29 pKa = 11.84 HH30 pKa = 4.7 PQVNQQLRR38 pKa = 11.84 LRR40 pKa = 11.84 AKK42 pKa = 9.89 AQRR45 pKa = 11.84 HH46 pKa = 4.66 PQVNQQLHH54 pKa = 4.5 QQAKK58 pKa = 8.04 VQLRR62 pKa = 11.84 QQVNQLL68 pKa = 3.23
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.193
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1490
0
1490
462731
38
1465
310.6
34.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.745 ± 0.079
0.442 ± 0.013
5.753 ± 0.065
5.377 ± 0.071
3.879 ± 0.039
6.743 ± 0.058
2.276 ± 0.027
6.519 ± 0.077
5.417 ± 0.057
9.936 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.815 ± 0.032
4.613 ± 0.057
3.872 ± 0.04
5.526 ± 0.079
4.163 ± 0.045
5.203 ± 0.048
6.504 ± 0.048
7.449 ± 0.058
1.211 ± 0.032
3.532 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here