Equus caballus papillomavirus 2
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0ITA7|C0ITA7_9PAPI Putative E1 OS=Equus caballus papillomavirus 2 OX=526413 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 GLHH5 pKa = 6.61 RR6 pKa = 11.84 LLPDD10 pKa = 3.28 VVLDD14 pKa = 3.92 ALSSITLAEE23 pKa = 4.16 RR24 pKa = 11.84 SPSVSSSDD32 pKa = 3.66 TEE34 pKa = 4.6 DD35 pKa = 5.42 EE36 pKa = 3.94 EE37 pKa = 4.45 DD38 pKa = 3.91 QPDD41 pKa = 3.62 GGAQRR46 pKa = 11.84 VAEE49 pKa = 4.27 EE50 pKa = 4.11 DD51 pKa = 3.86 LEE53 pKa = 4.79 VEE55 pKa = 4.62 VVPRR59 pKa = 11.84 EE60 pKa = 4.07 HH61 pKa = 6.19 PQFRR65 pKa = 11.84 VLTLCEE71 pKa = 3.68 RR72 pKa = 11.84 CNRR75 pKa = 11.84 GVRR78 pKa = 11.84 LVVVGPTGDD87 pKa = 3.54 VRR89 pKa = 11.84 ILQQLLLGSLGIVCADD105 pKa = 3.76 CASQEE110 pKa = 3.93 GHH112 pKa = 6.38 NGHH115 pKa = 6.12 GQQ117 pKa = 3.03
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.651
IPC2_protein 4.647
IPC_protein 4.546
Toseland 4.406
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.368
Rodwell 4.393
Grimsley 4.317
Solomon 4.495
Lehninger 4.444
Nozaki 4.609
DTASelect 4.762
Thurlkill 4.406
EMBOSS 4.393
Sillero 4.673
Patrickios 3.897
IPC_peptide 4.507
IPC2_peptide 4.66
IPC2.peptide.svr19 4.619
Protein with the highest isoelectric point:
>tr|C0ITA6|C0ITA6_9PAPI Protein E7 OS=Equus caballus papillomavirus 2 OX=526413 GN=E7 PE=3 SV=1
MM1 pKa = 7.98 PSRR4 pKa = 11.84 PVPAKK9 pKa = 10.79 SFGALFWLLWRR20 pKa = 11.84 CTQPICRR27 pKa = 11.84 LGLCEE32 pKa = 3.89 SANPLRR38 pKa = 11.84 CTEE41 pKa = 3.89 PFSRR45 pKa = 11.84 EE46 pKa = 4.17 DD47 pKa = 3.27 CCGPQSLRR55 pKa = 11.84 YY56 pKa = 9.88 AGGPCIGDD64 pKa = 3.31 VPRR67 pKa = 11.84 SSRR70 pKa = 11.84 GRR72 pKa = 11.84 VKK74 pKa = 9.99 TAGHH78 pKa = 5.6 MSIVGLLCIFCGKK91 pKa = 8.88 PCGYY95 pKa = 10.94 ADD97 pKa = 5.19 DD98 pKa = 4.54 IWWNTADD105 pKa = 4.37 LNLVMRR111 pKa = 11.84 DD112 pKa = 3.6 GKK114 pKa = 10.76 RR115 pKa = 11.84 KK116 pKa = 9.8 ASCQTCMRR124 pKa = 11.84 AACRR128 pKa = 11.84 LEE130 pKa = 3.56 RR131 pKa = 11.84 HH132 pKa = 6.6 LYY134 pKa = 8.19 QSSRR138 pKa = 11.84 NVYY141 pKa = 6.53 TARR144 pKa = 11.84 GVEE147 pKa = 4.29 RR148 pKa = 11.84 ACGQSVYY155 pKa = 10.04 RR156 pKa = 11.84 VSVRR160 pKa = 11.84 CTHH163 pKa = 6.09 CGKK166 pKa = 10.53 RR167 pKa = 11.84 LTRR170 pKa = 11.84 DD171 pKa = 3.06 EE172 pKa = 4.38 KK173 pKa = 10.7 RR174 pKa = 11.84 WIEE177 pKa = 3.65 IEE179 pKa = 3.98 GAGFYY184 pKa = 8.33 QVRR187 pKa = 11.84 QRR189 pKa = 11.84 WRR191 pKa = 11.84 ALCYY195 pKa = 10.07 DD196 pKa = 4.1 CRR198 pKa = 11.84 VCDD201 pKa = 3.88 EE202 pKa = 4.58 GSAA205 pKa = 3.53
Molecular weight: 23.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.456
IPC2_protein 8.244
IPC_protein 8.039
Toseland 7.454
ProMoST 8.639
Dawson 8.726
Bjellqvist 9.224
Wikipedia 8.639
Rodwell 8.756
Grimsley 7.38
Solomon 8.799
Lehninger 8.814
Nozaki 9.531
DTASelect 8.858
Thurlkill 8.902
EMBOSS 8.916
Sillero 9.282
Patrickios 4.062
IPC_peptide 8.785
IPC2_peptide 9.004
IPC2.peptide.svr19 9.253
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2200
117
519
366.7
40.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.045 ± 0.553
2.5 ± 0.909
5.591 ± 0.282
5.227 ± 0.406
4.591 ± 0.54
9.318 ± 1.718
2.5 ± 0.367
2.409 ± 0.409
4.091 ± 0.909
8.455 ± 0.719
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.548
3.227 ± 0.745
6.682 ± 0.604
4.182 ± 0.215
7.227 ± 0.68
7.636 ± 0.778
5.182 ± 0.262
7.955 ± 0.781
1.455 ± 0.454
2.5 ± 0.438
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here