Torque teno midi virus
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLX2|A7VLX2_9VIRU Capsid protein OS=Torque teno midi virus OX=432261 PE=3 SV=1
MM1 pKa = 7.38 QNICAKK7 pKa = 10.8 DD8 pKa = 3.5 FFTPTNFNCDD18 pKa = 3.24 TKK20 pKa = 10.8 NQLWMSIIADD30 pKa = 3.76 SHH32 pKa = 7.9 DD33 pKa = 4.39 SICNCWHH40 pKa = 7.17 PFAHH44 pKa = 6.23 MLASIFPPGHH54 pKa = 6.28 SDD56 pKa = 3.26 RR57 pKa = 11.84 NHH59 pKa = 6.24 TIEE62 pKa = 5.62 EE63 pKa = 4.0 ILQRR67 pKa = 11.84 DD68 pKa = 3.91 YY69 pKa = 11.59 KK70 pKa = 10.26 EE71 pKa = 4.2 RR72 pKa = 11.84 CHH74 pKa = 8.07 SGGHH78 pKa = 6.54 ADD80 pKa = 3.86 ASHH83 pKa = 6.24 GMEE86 pKa = 4.9 DD87 pKa = 3.22 SGAATGTDD95 pKa = 3.64 PIKK98 pKa = 10.72 RR99 pKa = 11.84 EE100 pKa = 3.9 EE101 pKa = 4.42 GEE103 pKa = 4.43 EE104 pKa = 3.83 EE105 pKa = 4.1 DD106 pKa = 4.94 LPEE109 pKa = 4.19 EE110 pKa = 4.58 EE111 pKa = 4.73 ISKK114 pKa = 10.53 LLAAAAEE121 pKa = 4.3 EE122 pKa = 4.83 GTRR125 pKa = 3.81
Molecular weight: 13.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.652
IPC2_protein 4.711
IPC_protein 4.635
Toseland 4.533
ProMoST 4.762
Dawson 4.609
Bjellqvist 4.749
Wikipedia 4.469
Rodwell 4.507
Grimsley 4.457
Solomon 4.609
Lehninger 4.558
Nozaki 4.724
DTASelect 4.863
Thurlkill 4.533
EMBOSS 4.507
Sillero 4.787
Patrickios 3.605
IPC_peptide 4.609
IPC2_peptide 4.787
IPC2.peptide.svr19 4.666
Protein with the highest isoelectric point:
>tr|A7VLX1|A7VLX1_9VIRU Uncharacterized protein OS=Torque teno midi virus OX=432261 PE=4 SV=1
MM1 pKa = 7.38 QNICAKK7 pKa = 10.8 DD8 pKa = 3.5 FFTPTNFNCDD18 pKa = 3.24 TKK20 pKa = 10.8 NQLWMSIIADD30 pKa = 3.76 SHH32 pKa = 7.9 DD33 pKa = 4.39 SICNCWHH40 pKa = 7.17 PFAHH44 pKa = 6.23 MLASIFPPGHH54 pKa = 6.28 SDD56 pKa = 3.26 RR57 pKa = 11.84 NHH59 pKa = 6.24 TIEE62 pKa = 5.62 EE63 pKa = 4.0 ILQRR67 pKa = 11.84 DD68 pKa = 3.91 YY69 pKa = 11.59 KK70 pKa = 10.26 EE71 pKa = 4.2 RR72 pKa = 11.84 CHH74 pKa = 8.07 SGGHH78 pKa = 6.54 ADD80 pKa = 3.86 ASHH83 pKa = 6.24 GMEE86 pKa = 4.9 DD87 pKa = 3.22 SGAATGTDD95 pKa = 3.64 PIKK98 pKa = 10.72 RR99 pKa = 11.84 EE100 pKa = 3.9 EE101 pKa = 4.42 GEE103 pKa = 4.43 EE104 pKa = 3.83 EE105 pKa = 4.1 DD106 pKa = 4.94 LPEE109 pKa = 4.19 EE110 pKa = 4.58 EE111 pKa = 4.73 ISKK114 pKa = 10.53 LLAAAAEE121 pKa = 4.33 EE122 pKa = 4.72 GTSGVAHH129 pKa = 7.18 KK130 pKa = 10.11 QQIHH134 pKa = 5.05 QLMTQNYY141 pKa = 7.29 NQHH144 pKa = 5.1 MMYY147 pKa = 9.85 PVCSKK152 pKa = 10.51 KK153 pKa = 10.2 QYY155 pKa = 10.8 KK156 pKa = 10.25 LRR158 pKa = 11.84 IQKK161 pKa = 9.45 NKK163 pKa = 8.3 PQNPFSTPGTSEE175 pKa = 3.63 GGLLHH180 pKa = 7.35 KK181 pKa = 10.32 EE182 pKa = 3.96 LLKK185 pKa = 10.75 EE186 pKa = 4.03 CQKK189 pKa = 10.98 ASKK192 pKa = 10.19 LIHH195 pKa = 6.65 LSNLMIQKK203 pKa = 9.17 HH204 pKa = 3.64 QRR206 pKa = 11.84 KK207 pKa = 8.99 RR208 pKa = 11.84 KK209 pKa = 9.06 RR210 pKa = 11.84 SPKK213 pKa = 9.82 HH214 pKa = 4.99 SQTTKK219 pKa = 10.22 SKK221 pKa = 9.67 RR222 pKa = 11.84 KK223 pKa = 8.85 RR224 pKa = 11.84 YY225 pKa = 9.79 RR226 pKa = 11.84 NVSSLSAKK234 pKa = 10.24 AISAKK239 pKa = 10.17 KK240 pKa = 10.06 KK241 pKa = 9.79 PPTSKK246 pKa = 9.24 STSSSSSSSSSSSNKK261 pKa = 9.11 TSTSSSSTSSS271 pKa = 2.93
Molecular weight: 30.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.897
IPC2_protein 8.58
IPC_protein 8.448
Toseland 9.399
ProMoST 9.063
Dawson 9.604
Bjellqvist 9.355
Wikipedia 9.692
Rodwell 10.101
Grimsley 9.604
Solomon 9.648
Lehninger 9.633
Nozaki 9.648
DTASelect 9.268
Thurlkill 9.502
EMBOSS 9.823
Sillero 9.619
Patrickios 7.629
IPC_peptide 9.648
IPC2_peptide 7.995
IPC2.peptide.svr19 7.868
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1319
125
665
329.8
37.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.217 ± 1.822
2.426 ± 0.26
4.625 ± 0.741
6.368 ± 1.137
4.397 ± 0.78
5.004 ± 0.292
3.26 ± 1.118
4.852 ± 0.353
8.567 ± 1.028
6.823 ± 0.825
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.971 ± 0.448
4.701 ± 0.22
6.672 ± 0.922
5.838 ± 0.938
5.914 ± 0.738
7.278 ± 2.794
6.596 ± 0.205
2.805 ± 1.099
1.82 ± 0.503
3.867 ± 1.461
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here