Natronolimnobius baerhuensis

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales;

Average proteome isoelectric point is 4.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202E5W2|A0A202E5W2_9EURY DNA repair and recombination protein RadA OS=Natronolimnobius baerhuensis OX=253108 GN=radA PE=3 SV=1
MM1 pKa = 7.61TGTTLDD7 pKa = 3.69YY8 pKa = 11.24HH9 pKa = 6.98HH10 pKa = 7.97DD11 pKa = 4.02SPSLRR16 pKa = 11.84IVDD19 pKa = 4.01ALAAVTDD26 pKa = 4.18TDD28 pKa = 5.03PLDD31 pKa = 4.75LDD33 pKa = 3.67PLYY36 pKa = 11.33NVVDD40 pKa = 4.69PEE42 pKa = 4.42ALDD45 pKa = 3.9QIVNSDD51 pKa = 3.51GDD53 pKa = 4.06QVDD56 pKa = 3.75HH57 pKa = 7.2LSVQFVYY64 pKa = 10.49DD65 pKa = 3.41GHH67 pKa = 6.69DD68 pKa = 3.3VQVRR72 pKa = 11.84GDD74 pKa = 3.38GSIAVDD80 pKa = 3.75GSVYY84 pKa = 11.18AHH86 pKa = 7.21GDD88 pKa = 3.16NN89 pKa = 4.47

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202E8W8|A0A202E8W8_9EURY Transposase OS=Natronolimnobius baerhuensis OX=253108 GN=B2G88_09800 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 2.66SRR5 pKa = 11.84LWIRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84VRR13 pKa = 11.84IWADD17 pKa = 2.78ALTYY21 pKa = 10.93ACTLTILAGVASGVLAIATGGGSVRR46 pKa = 11.84AKK48 pKa = 10.23EE49 pKa = 3.94LLFVTGWILLAYY61 pKa = 8.65ATIKK65 pKa = 10.25LWPTSPEE72 pKa = 3.88DD73 pKa = 3.86LEE75 pKa = 4.17EE76 pKa = 4.64DD77 pKa = 3.37RR78 pKa = 11.84TGDD81 pKa = 3.78SLADD85 pKa = 3.62SQPTTRR91 pKa = 11.84FQRR94 pKa = 11.84LVRR97 pKa = 11.84ALPPNRR103 pKa = 11.84WVRR106 pKa = 11.84LPRR109 pKa = 11.84PEE111 pKa = 4.89RR112 pKa = 11.84RR113 pKa = 11.84MTTAGKK119 pKa = 10.2VLLASVLILLVSFLLEE135 pKa = 3.99TVFGVTT141 pKa = 4.35

Molecular weight: 15.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3662

0

3662

1089211

28

3487

297.4

32.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.318 ± 0.05

0.766 ± 0.014

8.813 ± 0.062

9.166 ± 0.051

3.222 ± 0.025

7.971 ± 0.042

2.145 ± 0.019

4.683 ± 0.035

1.741 ± 0.026

8.996 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.018

2.463 ± 0.022

4.481 ± 0.026

2.828 ± 0.025

5.907 ± 0.04

5.762 ± 0.033

6.823 ± 0.036

8.268 ± 0.038

1.16 ± 0.017

2.734 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski