Bergeyella zoohelcum ATCC 43767
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K1MLF8|K1MLF8_9FLAO Uncharacterized protein OS=Bergeyella zoohelcum ATCC 43767 OX=883096 GN=HMPREF9699_01227 PE=4 SV=1
MM1 pKa = 7.73 AIIITDD7 pKa = 3.35 EE8 pKa = 4.75 CINCGACEE16 pKa = 4.03 PEE18 pKa = 4.51 CPNNAIYY25 pKa = 10.45 EE26 pKa = 4.24 GAIDD30 pKa = 3.9 WKK32 pKa = 11.13 ASDD35 pKa = 3.99 GTSLSGTVTFDD46 pKa = 3.45 SGLTVDD52 pKa = 5.19 ADD54 pKa = 3.87 APQEE58 pKa = 4.51 PISDD62 pKa = 3.77 DD63 pKa = 3.97 YY64 pKa = 11.95 YY65 pKa = 11.27 FIVPDD70 pKa = 3.79 KK71 pKa = 10.54 CTEE74 pKa = 3.89 CKK76 pKa = 10.46 GFHH79 pKa = 6.8 EE80 pKa = 5.19 EE81 pKa = 4.05 PQCAAVCPVDD91 pKa = 4.07 CCVPDD96 pKa = 3.7 EE97 pKa = 4.37 NRR99 pKa = 11.84 VEE101 pKa = 4.24 SEE103 pKa = 4.23 DD104 pKa = 3.55 VLLSRR109 pKa = 11.84 KK110 pKa = 9.79 AFLHH114 pKa = 6.77 AEE116 pKa = 4.08
Molecular weight: 12.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.617
ProMoST 3.935
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.935
Patrickios 0.172
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|K1M666|K1M666_9FLAO Uncharacterized protein OS=Bergeyella zoohelcum ATCC 43767 OX=883096 GN=HMPREF9699_00798 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.58 RR10 pKa = 11.84 KK11 pKa = 9.37 KK12 pKa = 10.2 RR13 pKa = 11.84 NKK15 pKa = 9.71 HH16 pKa = 4.09 GFRR19 pKa = 11.84 EE20 pKa = 4.33 RR21 pKa = 11.84 MSTPNGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.98 GRR39 pKa = 11.84 KK40 pKa = 8.81 SLTVSSARR48 pKa = 11.84 AKK50 pKa = 10.4 RR51 pKa = 3.45
Molecular weight: 5.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2120
0
2120
671449
20
2343
316.7
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.543 ± 0.047
0.73 ± 0.015
4.999 ± 0.035
7.014 ± 0.059
5.244 ± 0.045
6.38 ± 0.049
2.063 ± 0.026
7.932 ± 0.056
8.224 ± 0.059
8.957 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.51 ± 0.025
5.844 ± 0.054
3.389 ± 0.031
3.867 ± 0.035
3.578 ± 0.036
6.054 ± 0.043
5.495 ± 0.043
6.059 ± 0.044
1.017 ± 0.019
4.101 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here