Ovis aries papillomavirus 3
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5FL27|D5FL27_9PAPI Replication protein E1 OS=Ovis aries papillomavirus 3 OX=634772 GN=E1 PE=3 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 GQQPTLPDD10 pKa = 3.28 IVLHH14 pKa = 5.93 EE15 pKa = 4.31 VPEE18 pKa = 4.3 PVSLYY23 pKa = 10.5 CDD25 pKa = 3.35 EE26 pKa = 4.14 QLEE29 pKa = 4.4 EE30 pKa = 4.63 EE31 pKa = 4.56 EE32 pKa = 4.58 EE33 pKa = 4.24 QPALYY38 pKa = 9.49 TLYY41 pKa = 9.52 TLCGRR46 pKa = 11.84 CCQRR50 pKa = 11.84 LKK52 pKa = 10.18 LTFRR56 pKa = 11.84 AFTQTKK62 pKa = 8.05 ITLEE66 pKa = 4.0 TLLLGDD72 pKa = 5.39 LDD74 pKa = 5.14 LFCPPCAAA82 pKa = 4.79
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.257
IPC2_protein 4.495
IPC_protein 4.317
Toseland 4.177
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.113
Rodwell 4.164
Grimsley 4.088
Solomon 4.253
Lehninger 4.202
Nozaki 4.38
DTASelect 4.469
Thurlkill 4.19
EMBOSS 4.139
Sillero 4.431
Patrickios 0.604
IPC_peptide 4.253
IPC2_peptide 4.418
IPC2.peptide.svr19 4.346
Protein with the highest isoelectric point:
>tr|D8L564|D8L564_9PAPI E4 OS=Ovis aries papillomavirus 3 OX=634772 PE=4 SV=1
MM1 pKa = 7.28 AVWVPNGKK9 pKa = 9.27 SFYY12 pKa = 10.04 LPQSAVTRR20 pKa = 11.84 ILSTNEE26 pKa = 3.52 YY27 pKa = 9.16 VQGTGLVFHH36 pKa = 6.96 GSSNRR41 pKa = 11.84 LLCVGHH47 pKa = 6.9 PFYY50 pKa = 10.17 EE51 pKa = 4.37 TQRR54 pKa = 11.84 PDD56 pKa = 3.18 GSVKK60 pKa = 9.91 VPKK63 pKa = 10.3 VSSSQYY69 pKa = 10.37 RR70 pKa = 11.84 VFKK73 pKa = 11.09 VLLPDD78 pKa = 3.88 PNKK81 pKa = 10.18 FVFSEE86 pKa = 4.19 PNLYY90 pKa = 10.63 DD91 pKa = 3.87 PEE93 pKa = 4.28 SQRR96 pKa = 11.84 LVWKK100 pKa = 10.51 LRR102 pKa = 11.84 GLQVDD107 pKa = 3.96 RR108 pKa = 11.84 GQPHH112 pKa = 5.92 GVWVTGHH119 pKa = 7.18 LLMNKK124 pKa = 9.87 LDD126 pKa = 3.72 DD127 pKa = 3.97 TEE129 pKa = 4.15 NLGRR133 pKa = 11.84 QGSDD137 pKa = 2.75 PAGRR141 pKa = 11.84 DD142 pKa = 2.73 KK143 pKa = 11.18 DD144 pKa = 3.74 SRR146 pKa = 11.84 VNMGLEE152 pKa = 4.18 PKK154 pKa = 10.2 QMQVLIVGCRR164 pKa = 11.84 PPWGEE169 pKa = 3.46 HH170 pKa = 3.89 WGVAKK175 pKa = 10.5 KK176 pKa = 10.25 CASDD180 pKa = 3.51 NADD183 pKa = 3.67 PDD185 pKa = 3.52 KK186 pKa = 11.44 CPAIEE191 pKa = 4.26 LKK193 pKa = 9.63 STIIEE198 pKa = 4.84 DD199 pKa = 3.88 GNMMDD204 pKa = 3.83 TGFGNLDD211 pKa = 3.34 FRR213 pKa = 11.84 SLQEE217 pKa = 4.11 NKK219 pKa = 10.11 ADD221 pKa = 3.87 APIDD225 pKa = 3.45 ICQSICKK232 pKa = 9.64 YY233 pKa = 10.18 PDD235 pKa = 4.81 FIRR238 pKa = 11.84 MSQEE242 pKa = 3.74 TYY244 pKa = 9.06 GDD246 pKa = 3.5 HH247 pKa = 6.82 MFFCAKK253 pKa = 10.17 HH254 pKa = 4.23 EE255 pKa = 4.23 QIYY258 pKa = 9.73 LRR260 pKa = 11.84 HH261 pKa = 5.69 YY262 pKa = 9.56 FSKK265 pKa = 10.5 AGKK268 pKa = 9.71 IGEE271 pKa = 4.39 EE272 pKa = 4.35 VPKK275 pKa = 9.84 TLYY278 pKa = 10.62 VPPQPDD284 pKa = 3.68 TVNGTVNFWGSPSGSMVSSNNQLFNKK310 pKa = 9.08 PYY312 pKa = 9.77 WVRR315 pKa = 11.84 QAQGHH320 pKa = 5.67 NNGVLWNNLAFITVGDD336 pKa = 3.98 TTRR339 pKa = 11.84 GTNFNISVLDD349 pKa = 4.12 NGAQPYY355 pKa = 9.88 KK356 pKa = 10.24 DD357 pKa = 3.14 SSYY360 pKa = 11.78 AEE362 pKa = 4.21 FLRR365 pKa = 11.84 HH366 pKa = 5.81 VEE368 pKa = 4.1 EE369 pKa = 4.82 FDD371 pKa = 3.12 IQIIVEE377 pKa = 4.1 ACIVDD382 pKa = 4.23 LTPEE386 pKa = 3.88 IVSFIHH392 pKa = 6.7 QMDD395 pKa = 3.82 PTILDD400 pKa = 3.32 NWNLGIQAAPDD411 pKa = 3.31 SSLWEE416 pKa = 4.06 TYY418 pKa = 10.12 RR419 pKa = 11.84 YY420 pKa = 9.62 ISSFATKK427 pKa = 10.52 CPDD430 pKa = 3.29 QVPKK434 pKa = 10.78 PEE436 pKa = 4.33 APKK439 pKa = 10.74 DD440 pKa = 3.7 PYY442 pKa = 10.98 EE443 pKa = 4.64 KK444 pKa = 10.74 LSFWTVDD451 pKa = 3.58 LNEE454 pKa = 5.17 KK455 pKa = 10.28 LSQDD459 pKa = 3.43 LTHH462 pKa = 6.81 FPLGRR467 pKa = 11.84 RR468 pKa = 11.84 YY469 pKa = 10.06 LFQYY473 pKa = 7.15 TVRR476 pKa = 11.84 PPKK479 pKa = 10.48 SAVKK483 pKa = 10.32 RR484 pKa = 11.84 KK485 pKa = 9.1 AANNSGLNSSKK496 pKa = 10.35 RR497 pKa = 11.84 RR498 pKa = 11.84 RR499 pKa = 11.84 KK500 pKa = 10.11 LNNKK504 pKa = 8.86
Molecular weight: 57.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.815
IPC2_protein 7.102
IPC_protein 7.059
Toseland 6.898
ProMoST 7.702
Dawson 7.717
Bjellqvist 7.966
Wikipedia 7.644
Rodwell 7.732
Grimsley 6.985
Solomon 7.775
Lehninger 7.79
Nozaki 8.2
DTASelect 7.878
Thurlkill 7.922
EMBOSS 7.966
Sillero 8.2
Patrickios 4.291
IPC_peptide 7.775
IPC2_peptide 7.234
IPC2.peptide.svr19 7.234
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2362
82
607
337.4
37.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.588 ± 0.507
2.456 ± 0.618
5.8 ± 0.567
6.732 ± 0.84
3.683 ± 0.508
6.647 ± 0.938
2.244 ± 0.184
3.895 ± 0.361
5.207 ± 0.745
8.552 ± 0.591
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.651 ± 0.269
4.149 ± 0.617
6.774 ± 0.829
4.742 ± 0.552
5.97 ± 0.59
7.917 ± 0.763
6.181 ± 0.742
6.901 ± 0.664
1.82 ± 0.345
3.091 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here