Kadipiro virus
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9YWP6|Q9YWP6_9REOV Vp8 OS=Kadipiro virus OX=104580 PE=4 SV=1
MM1 pKa = 7.57 SNIHH5 pKa = 6.96 CYY7 pKa = 10.71 GSDD10 pKa = 3.19 PSEE13 pKa = 3.75 GHH15 pKa = 5.19 FCIYY19 pKa = 10.3 RR20 pKa = 11.84 FDD22 pKa = 3.73 EE23 pKa = 4.74 GQPFTVKK30 pKa = 10.24 FRR32 pKa = 11.84 QKK34 pKa = 11.03 GKK36 pKa = 8.66 IQHH39 pKa = 6.51 IEE41 pKa = 3.92 LEE43 pKa = 4.09 NSIGHH48 pKa = 6.4 NGAINYY54 pKa = 9.5 GSIFDD59 pKa = 4.17 TIKK62 pKa = 10.46 YY63 pKa = 5.94 FTSIVGLTLINNNMTIDD80 pKa = 4.02 LNSVAVDD87 pKa = 3.06 NRR89 pKa = 11.84 FRR91 pKa = 11.84 GNATMVALKK100 pKa = 10.85 CNVLGNLFLKK110 pKa = 10.67 KK111 pKa = 9.87 IVSSDD116 pKa = 3.29 LQLTLKK122 pKa = 10.54 FYY124 pKa = 10.46 YY125 pKa = 10.14 NKK127 pKa = 10.16 QIRR130 pKa = 11.84 YY131 pKa = 9.22 DD132 pKa = 3.72 VFAGMYY138 pKa = 10.57 GLINLRR144 pKa = 11.84 NSKK147 pKa = 10.61 FNDD150 pKa = 2.77 NAYY153 pKa = 10.51 NYY155 pKa = 10.55 GIIIEE160 pKa = 4.1 QCYY163 pKa = 9.2 PIKK166 pKa = 10.29 DD167 pKa = 3.56 LPMNKK172 pKa = 8.87 ILAGLKK178 pKa = 10.33 ALMLFHH184 pKa = 7.03 EE185 pKa = 5.01 HH186 pKa = 6.16 TNCIHH191 pKa = 6.91 GDD193 pKa = 3.96 CNPSNIMCDD202 pKa = 2.74 KK203 pKa = 9.19 MGNVKK208 pKa = 9.99 IVDD211 pKa = 3.76 PASLVTRR218 pKa = 11.84 VVTYY222 pKa = 10.47 INEE225 pKa = 4.24 YY226 pKa = 10.8 YY227 pKa = 10.58 KK228 pKa = 11.0 DD229 pKa = 3.92 LTPKK233 pKa = 10.39 SEE235 pKa = 3.85 VGAYY239 pKa = 10.09 LLSCFEE245 pKa = 4.27 IVSDD249 pKa = 4.56 LRR251 pKa = 11.84 KK252 pKa = 10.11 LPLEE256 pKa = 4.35 KK257 pKa = 10.18 IFIKK261 pKa = 10.29 RR262 pKa = 11.84 GYY264 pKa = 10.22 LGLPEE269 pKa = 4.22 YY270 pKa = 11.08 ADD272 pKa = 4.27 DD273 pKa = 4.77 GGINAIDD280 pKa = 4.3 FLTSLQDD287 pKa = 3.45 GLTTHH292 pKa = 6.96 SDD294 pKa = 3.29 LMSAIAGVPFDD305 pKa = 4.99 GLLANNEE312 pKa = 4.37 YY313 pKa = 10.21 IDD315 pKa = 6.08 DD316 pKa = 4.48 DD317 pKa = 5.67 LSDD320 pKa = 3.89 NEE322 pKa = 4.82 CNVNLDD328 pKa = 3.56 AGIDD332 pKa = 3.48 NLEE335 pKa = 4.11 FNNLDD340 pKa = 3.39 VDD342 pKa = 4.73 DD343 pKa = 5.38 SDD345 pKa = 5.54 SEE347 pKa = 4.3
Molecular weight: 38.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.567
IPC2_protein 4.736
IPC_protein 4.711
Toseland 4.571
ProMoST 4.813
Dawson 4.698
Bjellqvist 4.863
Wikipedia 4.609
Rodwell 4.571
Grimsley 4.482
Solomon 4.698
Lehninger 4.66
Nozaki 4.813
DTASelect 5.041
Thurlkill 4.584
EMBOSS 4.635
Sillero 4.863
Patrickios 3.045
IPC_peptide 4.698
IPC2_peptide 4.851
IPC2.peptide.svr19 4.775
Protein with the highest isoelectric point:
>tr|Q9YYS1|Q9YYS1_9REOV Vp12 OS=Kadipiro virus OX=104580 PE=4 SV=1
MM1 pKa = 7.61 SNARR5 pKa = 11.84 YY6 pKa = 10.52 NMISAVSASGCKK18 pKa = 9.02 NDD20 pKa = 5.41 KK21 pKa = 9.72 PFCVHH26 pKa = 6.18 KK27 pKa = 10.23 VVAVIVIIVSSGITVVNQIGQRR49 pKa = 11.84 KK50 pKa = 9.27 LSTDD54 pKa = 3.39 DD55 pKa = 3.91 YY56 pKa = 11.18 FVLSLVCDD64 pKa = 5.21 AISNIIVVGLSVVGMTASSKK84 pKa = 9.13 TKK86 pKa = 10.33 QVLTDD91 pKa = 3.42 GGEE94 pKa = 3.95 NAIEE98 pKa = 4.13 LNHH101 pKa = 5.4 MPKK104 pKa = 10.3 RR105 pKa = 11.84 IVKK108 pKa = 10.16 KK109 pKa = 10.41 LLKK112 pKa = 10.32 EE113 pKa = 3.81 EE114 pKa = 4.39 AKK116 pKa = 10.99 NGVDD120 pKa = 4.03 FVKK123 pKa = 10.91 SIGKK127 pKa = 8.91 DD128 pKa = 3.12 LKK130 pKa = 11.61 SNDD133 pKa = 3.66 NKK135 pKa = 10.2 PDD137 pKa = 3.49 PSAPPKK143 pKa = 10.14 YY144 pKa = 10.19 EE145 pKa = 4.68 KK146 pKa = 9.66 IDD148 pKa = 3.41 EE149 pKa = 4.27 SFEE152 pKa = 4.34 LKK154 pKa = 10.62 SEE156 pKa = 4.19 FAGKK160 pKa = 10.16 SLITDD165 pKa = 3.07 NRR167 pKa = 11.84 TGQSFIVTSDD177 pKa = 3.23 LVKK180 pKa = 10.48 PLILEE185 pKa = 4.23 SRR187 pKa = 11.84 SQSS190 pKa = 3.15
Molecular weight: 20.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.056
IPC2_protein 8.053
IPC_protein 8.024
Toseland 8.639
ProMoST 8.521
Dawson 8.931
Bjellqvist 8.902
Wikipedia 8.946
Rodwell 9.165
Grimsley 8.478
Solomon 9.136
Lehninger 9.136
Nozaki 9.194
DTASelect 8.756
Thurlkill 8.931
EMBOSS 9.165
Sillero 9.136
Patrickios 5.194
IPC_peptide 9.136
IPC2_peptide 7.527
IPC2.peptide.svr19 7.638
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
6305
190
1223
525.4
58.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.376 ± 0.457
1.427 ± 0.199
6.693 ± 0.322
5.155 ± 0.27
3.759 ± 0.263
5.583 ± 0.203
2.094 ± 0.228
7.407 ± 0.298
6.233 ± 0.468
9.437 ± 0.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.223
6.503 ± 0.508
3.236 ± 0.248
2.205 ± 0.138
5.044 ± 0.46
8.374 ± 0.448
5.757 ± 0.293
7.423 ± 0.533
0.508 ± 0.078
4.171 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here