Butcherbird polyomavirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Gammapolyomavirus; Cracticus torquatus polyomavirus 1

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5K7E5|V5K7E5_9POLY Capsid protein VP1 OS=Butcherbird polyomavirus OX=1394033 GN=VP1 PE=3 SV=1
MM1 pKa = 7.66DD2 pKa = 4.26TDD4 pKa = 3.93GTAVPAAAGTEE15 pKa = 4.18QPEE18 pKa = 4.39NRR20 pKa = 11.84TPRR23 pKa = 11.84PPTPGPSAAGEE34 pKa = 4.3TPKK37 pKa = 11.0ASGPSRR43 pKa = 11.84DD44 pKa = 3.51RR45 pKa = 11.84TDD47 pKa = 3.4GNTGSNSTGSTSHH60 pKa = 5.93TKK62 pKa = 10.36EE63 pKa = 3.94SADD66 pKa = 3.6TGVSPAGKK74 pKa = 9.22PPGSPPLTQPDD85 pKa = 4.36NLTPPASPHH94 pKa = 6.93DD95 pKa = 4.1GMDD98 pKa = 3.05VDD100 pKa = 5.12EE101 pKa = 5.09NQPSPAYY108 pKa = 9.74EE109 pKa = 4.46GSCTCTFTLKK119 pKa = 10.47VGPKK123 pKa = 8.46ICEE126 pKa = 4.12EE127 pKa = 3.96CRR129 pKa = 11.84HH130 pKa = 5.42WLMSDD135 pKa = 3.15TDD137 pKa = 3.52

Molecular weight: 14.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5K6X6|V5K6X6_9POLY Large T antigen OS=Butcherbird polyomavirus OX=1394033 PE=4 SV=1
MM1 pKa = 7.43ALEE4 pKa = 4.46VWQPPAIDD12 pKa = 3.45ILFPGATEE20 pKa = 3.88IANGIYY26 pKa = 10.22SLNPLEE32 pKa = 4.61WGPSLFNRR40 pKa = 11.84IAQYY44 pKa = 11.04LWDD47 pKa = 4.61SIITTGRR54 pKa = 11.84RR55 pKa = 11.84QIGMATQAATQQAASTLYY73 pKa = 10.9DD74 pKa = 3.88LAARR78 pKa = 11.84AAEE81 pKa = 3.88NARR84 pKa = 11.84WYY86 pKa = 10.72VIEE89 pKa = 4.43TPSRR93 pKa = 11.84TYY95 pKa = 10.48QALKK99 pKa = 10.35DD100 pKa = 3.89YY101 pKa = 9.87YY102 pKa = 10.13SQVSVLPPNARR113 pKa = 11.84RR114 pKa = 11.84GRR116 pKa = 11.84ALFLADD122 pKa = 3.97EE123 pKa = 4.66EE124 pKa = 4.57EE125 pKa = 4.97EE126 pKa = 4.03ILKK129 pKa = 9.63PQDD132 pKa = 3.41PDD134 pKa = 2.99ISGEE138 pKa = 4.09SVYY141 pKa = 10.75RR142 pKa = 11.84EE143 pKa = 4.08EE144 pKa = 5.3APGGAQQRR152 pKa = 11.84TCPDD156 pKa = 2.4WMLPLILGLYY166 pKa = 7.05GTVYY170 pKa = 10.12PGWRR174 pKa = 11.84AEE176 pKa = 4.18VNLLEE181 pKa = 4.33QNGTQSSQKK190 pKa = 7.27VQRR193 pKa = 11.84RR194 pKa = 11.84RR195 pKa = 11.84SRR197 pKa = 11.84PRR199 pKa = 11.84TSAQAPYY206 pKa = 10.31QRR208 pKa = 11.84RR209 pKa = 11.84NRR211 pKa = 11.84SSRR214 pKa = 11.84SKK216 pKa = 10.59NRR218 pKa = 11.84SRR220 pKa = 4.26

Molecular weight: 24.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1855

137

640

309.2

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.38 ± 1.296

1.941 ± 0.593

5.175 ± 0.621

6.523 ± 0.332

2.911 ± 0.564

7.17 ± 0.811

1.509 ± 0.392

4.259 ± 0.571

4.636 ± 0.998

10.189 ± 0.846

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.941 ± 0.197

3.881 ± 0.413

6.954 ± 1.154

4.906 ± 0.822

5.768 ± 0.777

7.763 ± 0.543

5.876 ± 0.796

4.744 ± 0.748

1.024 ± 0.311

3.45 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski