Vibrio phage douglas 12A4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4M9L2|M4M9L2_9CAUD Uncharacterized protein OS=Vibrio phage douglas 12A4 OX=573171 GN=VPAG_00052 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 10.5YY3 pKa = 10.69LIISFLLISGCTINLQIDD21 pKa = 3.5TDD23 pKa = 4.14YY24 pKa = 11.56AAQRR28 pKa = 11.84NMDD31 pKa = 3.56GTILEE36 pKa = 4.79TIDD39 pKa = 3.69TEE41 pKa = 4.67TTSSITPTTDD51 pKa = 3.2LEE53 pKa = 4.42VDD55 pKa = 3.46SGALL59 pKa = 3.33

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4MCK2|M4MCK2_9CAUD Uncharacterized protein OS=Vibrio phage douglas 12A4 OX=573171 GN=VPAG_00030 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84EE3 pKa = 3.6QRR5 pKa = 11.84IIRR8 pKa = 11.84LTSNQIEE15 pKa = 4.53ILNQVKK21 pKa = 8.27TFYY24 pKa = 10.2IEE26 pKa = 4.01HH27 pKa = 6.57SKK29 pKa = 11.24LPTCKK34 pKa = 9.99EE35 pKa = 3.91LAKK38 pKa = 10.74HH39 pKa = 6.0SGRR42 pKa = 11.84TMSSAARR49 pKa = 11.84GLEE52 pKa = 3.77ALIKK56 pKa = 10.55AGIIINITQIKK67 pKa = 9.36KK68 pKa = 8.05RR69 pKa = 11.84WTRR72 pKa = 11.84IPYY75 pKa = 8.7TVEE78 pKa = 3.58DD79 pKa = 3.27TGRR82 pKa = 11.84WINEE86 pKa = 4.06RR87 pKa = 11.84IVKK90 pKa = 10.39AKK92 pKa = 10.44

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

18251

41

3026

243.3

27.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.164 ± 0.419

0.975 ± 0.184

6.728 ± 0.305

7.232 ± 0.387

3.463 ± 0.183

6.712 ± 0.273

1.583 ± 0.162

6.591 ± 0.307

7.764 ± 0.256

7.572 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.185

5.271 ± 0.234

3.529 ± 0.154

4.076 ± 0.273

4.076 ± 0.338

6.843 ± 0.289

6.087 ± 0.312

6.18 ± 0.25

1.205 ± 0.091

3.282 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski