Ctenophore-associated circular genome 4
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJB6|A0A141MJB6_9VIRU ATP-dependent helicase Rep OS=Ctenophore-associated circular genome 4 OX=1778573 PE=3 SV=1
MM1 pKa = 7.4 VRR3 pKa = 11.84 EE4 pKa = 4.09 SAAVGWCFTLNNPTDD19 pKa = 3.79 EE20 pKa = 4.61 EE21 pKa = 4.42 VDD23 pKa = 3.44 NLNRR27 pKa = 11.84 ILDD30 pKa = 4.04 GDD32 pKa = 3.9 QIKK35 pKa = 10.32 YY36 pKa = 10.59 AIYY39 pKa = 10.06 QKK41 pKa = 9.61 EE42 pKa = 4.18 TGTPHH47 pKa = 5.6 LQGYY51 pKa = 9.44 LEE53 pKa = 4.22 VSRR56 pKa = 11.84 KK57 pKa = 7.75 QRR59 pKa = 11.84 LVGVKK64 pKa = 10.1 RR65 pKa = 11.84 LLQTEE70 pKa = 4.27 RR71 pKa = 11.84 VHH73 pKa = 7.75 LEE75 pKa = 3.5 KK76 pKa = 10.81 RR77 pKa = 11.84 KK78 pKa = 8.48 GTRR81 pKa = 11.84 IQARR85 pKa = 11.84 DD86 pKa = 3.54 YY87 pKa = 10.96 CRR89 pKa = 11.84 KK90 pKa = 8.4 TEE92 pKa = 4.01 GRR94 pKa = 11.84 LADD97 pKa = 3.54 PVVFGVWRR105 pKa = 11.84 GDD107 pKa = 3.54 SQTTGMSRR115 pKa = 11.84 VIARR119 pKa = 11.84 LEE121 pKa = 4.39 GGCTLSEE128 pKa = 4.36 VAAEE132 pKa = 4.19 EE133 pKa = 4.22 PQTFIQYY140 pKa = 10.77 HH141 pKa = 5.66 NGLAKK146 pKa = 9.92 WYY148 pKa = 10.59 ALVCTFARR156 pKa = 11.84 EE157 pKa = 3.94 TDD159 pKa = 3.42 SPRR162 pKa = 11.84 GVWIYY167 pKa = 10.6 GPPGIGKK174 pKa = 5.65 THH176 pKa = 7.62 KK177 pKa = 10.51 ARR179 pKa = 11.84 ALASEE184 pKa = 4.07 SLYY187 pKa = 11.13 LKK189 pKa = 10.32 SQNKK193 pKa = 7.34 WWDD196 pKa = 3.47 GYY198 pKa = 11.17 AGQKK202 pKa = 10.02 FVVLDD207 pKa = 3.81 DD208 pKa = 4.44 FDD210 pKa = 4.23 RR211 pKa = 11.84 QGICLGHH218 pKa = 5.82 YY219 pKa = 10.12 LKK221 pKa = 10.23 IWSDD225 pKa = 2.95 KK226 pKa = 9.4 WSCTAEE232 pKa = 4.01 IKK234 pKa = 10.9 GGTVNLQHH242 pKa = 6.92 EE243 pKa = 4.76 KK244 pKa = 10.82 FIVTSNYY251 pKa = 9.94 HH252 pKa = 6.82 PDD254 pKa = 3.88 EE255 pKa = 4.77 LWTDD259 pKa = 3.62 DD260 pKa = 4.19 PQLVDD265 pKa = 4.73 AISRR269 pKa = 11.84 RR270 pKa = 11.84 FEE272 pKa = 4.34 IIRR275 pKa = 11.84 GEE277 pKa = 4.01 SDD279 pKa = 3.41 TNVTNWIVEE288 pKa = 4.11 NN289 pKa = 4.0
Molecular weight: 33.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.482
IPC2_protein 6.504
IPC_protein 6.664
Toseland 6.664
ProMoST 7.044
Dawson 7.146
Bjellqvist 7.044
Wikipedia 7.117
Rodwell 7.146
Grimsley 6.751
Solomon 7.19
Lehninger 7.205
Nozaki 7.38
DTASelect 7.439
Thurlkill 7.468
EMBOSS 7.497
Sillero 7.571
Patrickios 4.317
IPC_peptide 7.205
IPC2_peptide 7.059
IPC2.peptide.svr19 6.963
Protein with the highest isoelectric point:
>tr|A0A141MJB6|A0A141MJB6_9VIRU ATP-dependent helicase Rep OS=Ctenophore-associated circular genome 4 OX=1778573 PE=3 SV=1
MM1 pKa = 7.4 VRR3 pKa = 11.84 EE4 pKa = 4.09 SAAVGWCFTLNNPTDD19 pKa = 3.79 EE20 pKa = 4.61 EE21 pKa = 4.42 VDD23 pKa = 3.44 NLNRR27 pKa = 11.84 ILDD30 pKa = 4.04 GDD32 pKa = 3.9 QIKK35 pKa = 10.32 YY36 pKa = 10.59 AIYY39 pKa = 10.06 QKK41 pKa = 9.61 EE42 pKa = 4.18 TGTPHH47 pKa = 5.6 LQGYY51 pKa = 9.44 LEE53 pKa = 4.22 VSRR56 pKa = 11.84 KK57 pKa = 7.75 QRR59 pKa = 11.84 LVGVKK64 pKa = 10.1 RR65 pKa = 11.84 LLQTEE70 pKa = 4.27 RR71 pKa = 11.84 VHH73 pKa = 7.75 LEE75 pKa = 3.5 KK76 pKa = 10.81 RR77 pKa = 11.84 KK78 pKa = 8.48 GTRR81 pKa = 11.84 IQARR85 pKa = 11.84 DD86 pKa = 3.54 YY87 pKa = 10.96 CRR89 pKa = 11.84 KK90 pKa = 8.4 TEE92 pKa = 4.01 GRR94 pKa = 11.84 LADD97 pKa = 3.54 PVVFGVWRR105 pKa = 11.84 GDD107 pKa = 3.54 SQTTGMSRR115 pKa = 11.84 VIARR119 pKa = 11.84 LEE121 pKa = 4.39 GGCTLSEE128 pKa = 4.36 VAAEE132 pKa = 4.19 EE133 pKa = 4.22 PQTFIQYY140 pKa = 10.77 HH141 pKa = 5.66 NGLAKK146 pKa = 9.92 WYY148 pKa = 10.59 ALVCTFARR156 pKa = 11.84 EE157 pKa = 3.94 TDD159 pKa = 3.42 SPRR162 pKa = 11.84 GVWIYY167 pKa = 10.6 GPPGIGKK174 pKa = 5.65 THH176 pKa = 7.62 KK177 pKa = 10.51 ARR179 pKa = 11.84 ALASEE184 pKa = 4.07 SLYY187 pKa = 11.13 LKK189 pKa = 10.32 SQNKK193 pKa = 7.34 WWDD196 pKa = 3.47 GYY198 pKa = 11.17 AGQKK202 pKa = 10.02 FVVLDD207 pKa = 3.81 DD208 pKa = 4.44 FDD210 pKa = 4.23 RR211 pKa = 11.84 QGICLGHH218 pKa = 5.82 YY219 pKa = 10.12 LKK221 pKa = 10.23 IWSDD225 pKa = 2.95 KK226 pKa = 9.4 WSCTAEE232 pKa = 4.01 IKK234 pKa = 10.9 GGTVNLQHH242 pKa = 6.92 EE243 pKa = 4.76 KK244 pKa = 10.82 FIVTSNYY251 pKa = 9.94 HH252 pKa = 6.82 PDD254 pKa = 3.88 EE255 pKa = 4.77 LWTDD259 pKa = 3.62 DD260 pKa = 4.19 PQLVDD265 pKa = 4.73 AISRR269 pKa = 11.84 RR270 pKa = 11.84 FEE272 pKa = 4.34 IIRR275 pKa = 11.84 GEE277 pKa = 4.01 SDD279 pKa = 3.41 TNVTNWIVEE288 pKa = 4.11 NN289 pKa = 4.0
Molecular weight: 33.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.482
IPC2_protein 6.504
IPC_protein 6.664
Toseland 6.664
ProMoST 7.044
Dawson 7.146
Bjellqvist 7.044
Wikipedia 7.117
Rodwell 7.146
Grimsley 6.751
Solomon 7.19
Lehninger 7.205
Nozaki 7.38
DTASelect 7.439
Thurlkill 7.468
EMBOSS 7.497
Sillero 7.571
Patrickios 4.317
IPC_peptide 7.205
IPC2_peptide 7.059
IPC2.peptide.svr19 6.963
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
289
289
289
289.0
33.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.882 ± 0.0
2.076 ± 0.0
6.228 ± 0.0
6.92 ± 0.0
2.768 ± 0.0
8.304 ± 0.0
2.422 ± 0.0
5.536 ± 0.0
5.882 ± 0.0
7.958 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.692 ± 0.0
3.806 ± 0.0
3.114 ± 0.0
4.844 ± 0.0
7.266 ± 0.0
4.844 ± 0.0
6.92 ± 0.0
7.266 ± 0.0
3.46 ± 0.0
3.806 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here