Staphylococcus phage IME1365_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockefellervirus; unclassified Rockefellervirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JQL1|A0A1W6JQL1_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1365_01 OX=1965494 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.02IVYY5 pKa = 8.78LWKK8 pKa = 10.34NGQPVIVTTNEE19 pKa = 3.62EE20 pKa = 4.04GEE22 pKa = 4.19YY23 pKa = 10.09EE24 pKa = 4.29YY25 pKa = 10.3PSEE28 pKa = 4.29EE29 pKa = 3.94WTEE32 pKa = 4.08TKK34 pKa = 10.25PDD36 pKa = 3.66DD37 pKa = 4.65GMYY40 pKa = 9.95TPIYY44 pKa = 10.4FDD46 pKa = 3.38GQKK49 pKa = 10.11WIGQSKK55 pKa = 9.81EE56 pKa = 3.95VFEE59 pKa = 5.14KK60 pKa = 10.51EE61 pKa = 4.48LPPEE65 pKa = 4.78PIDD68 pKa = 4.46DD69 pKa = 3.96KK70 pKa = 11.76DD71 pKa = 4.07VIIANLSEE79 pKa = 4.13QLLNTQLEE87 pKa = 4.38IEE89 pKa = 4.27NVKK92 pKa = 10.76KK93 pKa = 10.97DD94 pKa = 3.35MATVLKK100 pKa = 10.59LLLEE104 pKa = 4.57NGSVDD109 pKa = 4.6DD110 pKa = 4.3VQNSS114 pKa = 3.17

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JQJ3|A0A1W6JQJ3_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1365_01 OX=1965494 PE=4 SV=1
MM1 pKa = 7.43ILSDD5 pKa = 4.94TINQRR10 pKa = 11.84YY11 pKa = 9.1RR12 pKa = 11.84YY13 pKa = 7.45ATQGKK18 pKa = 7.42TPTQIQQEE26 pKa = 4.18LRR28 pKa = 11.84KK29 pKa = 10.09LGVNGFVVKK38 pKa = 10.52VAGSRR43 pKa = 11.84VTMLVNEE50 pKa = 4.21NDD52 pKa = 2.94IKK54 pKa = 10.99RR55 pKa = 11.84NRR57 pKa = 11.84EE58 pKa = 3.67CVRR61 pKa = 11.84NGNRR65 pKa = 3.44

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13921

46

1070

221.0

25.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.919 ± 0.418

0.596 ± 0.104

6.451 ± 0.344

6.616 ± 0.518

3.994 ± 0.219

6.02 ± 0.368

1.731 ± 0.158

7.205 ± 0.243

8.951 ± 0.471

7.27 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.144

6.817 ± 0.339

3.103 ± 0.202

4.095 ± 0.284

4.339 ± 0.269

5.984 ± 0.273

6.487 ± 0.295

6.178 ± 0.345

1.358 ± 0.156

4.468 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski