Capybara microvirus Cap1_SP_142
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4D1|A0A4P8W4D1_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_142 OX=2585388 PE=3 SV=1
MM1 pKa = 7.43 TNQEE5 pKa = 3.78 KK6 pKa = 10.65 ALTFEE11 pKa = 4.54 YY12 pKa = 10.44 DD13 pKa = 3.17 GKK15 pKa = 8.84 TIKK18 pKa = 11.03 YY19 pKa = 9.86 GDD21 pKa = 4.18 PLYY24 pKa = 10.76 MFDD27 pKa = 5.76 LKK29 pKa = 10.66 WHH31 pKa = 6.89 DD32 pKa = 4.39 EE33 pKa = 4.12 KK34 pKa = 11.61 EE35 pKa = 3.97 MDD37 pKa = 3.5 YY38 pKa = 11.77 LMIVGVKK45 pKa = 8.82 PYY47 pKa = 10.65 CEE49 pKa = 4.7 AIEE52 pKa = 4.13 ARR54 pKa = 11.84 AKK56 pKa = 10.75 GVTPYY61 pKa = 11.02 EE62 pKa = 4.71 LIDD65 pKa = 3.59 TYY67 pKa = 11.68 GGIDD71 pKa = 3.35 NVEE74 pKa = 3.95 TVLPVKK80 pKa = 10.41 GQYY83 pKa = 10.69 LDD85 pKa = 3.46 VSEE88 pKa = 4.43 MPEE91 pKa = 3.69 IGNDD95 pKa = 3.43 LAYY98 pKa = 10.08 EE99 pKa = 4.01 QALNTLQAKK108 pKa = 10.18 LDD110 pKa = 3.87 EE111 pKa = 4.61 LKK113 pKa = 9.43 KK114 pKa = 9.29 TKK116 pKa = 10.42 LIQEE120 pKa = 4.48 EE121 pKa = 4.61 KK122 pKa = 10.5 PKK124 pKa = 11.15 EE125 pKa = 3.77 EE126 pKa = 4.61 DD127 pKa = 3.19 KK128 pKa = 11.57
Molecular weight: 14.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.529
IPC2_protein 4.546
IPC_protein 4.444
Toseland 4.279
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.24
Rodwell 4.279
Grimsley 4.19
Solomon 4.38
Lehninger 4.329
Nozaki 4.495
DTASelect 4.635
Thurlkill 4.291
EMBOSS 4.266
Sillero 4.546
Patrickios 3.719
IPC_peptide 4.38
IPC2_peptide 4.546
IPC2.peptide.svr19 4.485
Protein with the highest isoelectric point:
>tr|A0A4P8W7X3|A0A4P8W7X3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_142 OX=2585388 PE=4 SV=1
MM1 pKa = 6.68 KK2 pKa = 9.43 TQYY5 pKa = 10.86 FKK7 pKa = 11.28 INMLLAMSINRR18 pKa = 11.84 GIKK21 pKa = 9.67 AKK23 pKa = 10.01 RR24 pKa = 11.84 LCRR27 pKa = 11.84 EE28 pKa = 3.77 SYY30 pKa = 10.01 LYY32 pKa = 10.46 RR33 pKa = 11.84 VFVSMEE39 pKa = 3.87 NPRR42 pKa = 11.84 CPSVPLAGTLSTRR55 pKa = 11.84 ACPYY59 pKa = 10.8 ACAKK63 pKa = 9.96 LKK65 pKa = 9.17 KK66 pKa = 8.69 TRR68 pKa = 11.84 AEE70 pKa = 3.95 TSNASILL77 pKa = 3.77
Molecular weight: 8.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.575
IPC_protein 9.75
Toseland 10.233
ProMoST 9.911
Dawson 10.409
Bjellqvist 10.116
Wikipedia 10.584
Rodwell 10.847
Grimsley 10.482
Solomon 10.452
Lehninger 10.423
Nozaki 10.292
DTASelect 10.087
Thurlkill 10.277
EMBOSS 10.643
Sillero 10.35
Patrickios 10.599
IPC_peptide 10.452
IPC2_peptide 9.209
IPC2.peptide.svr19 8.378
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1522
77
551
190.3
21.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.819 ± 1.566
1.577 ± 0.526
5.256 ± 0.608
6.702 ± 1.077
4.599 ± 0.513
5.256 ± 0.599
2.037 ± 0.421
6.439 ± 0.601
7.819 ± 1.422
8.936 ± 1.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.825 ± 0.559
5.519 ± 0.702
4.796 ± 0.527
2.825 ± 0.218
4.008 ± 0.589
7.03 ± 1.068
6.176 ± 0.822
4.205 ± 0.582
0.92 ± 0.176
5.256 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here