Prunus dulcis (Almond) (Amygdalus dulcis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E4E4M8|A0A5E4E4M8_PRUDU Uncharacterized protein OS=Prunus dulcis OX=3755 GN=ALMOND_2B026615 PE=4 SV=1
MM1 pKa = 7.36FPLSKK6 pKa = 10.63LIISASWYY14 pKa = 8.29EE15 pKa = 3.88VHH17 pKa = 7.34GDD19 pKa = 4.04GVDD22 pKa = 3.92DD23 pKa = 5.12FNGDD27 pKa = 4.5DD28 pKa = 3.66IFALAACIEE37 pKa = 4.22GDD39 pKa = 4.59GDD41 pKa = 6.02DD42 pKa = 5.53DD43 pKa = 6.4DD44 pKa = 6.51SDD46 pKa = 4.13YY47 pKa = 11.41DD48 pKa = 3.78YY49 pKa = 11.73APAAMEE55 pKa = 5.19GDD57 pKa = 5.03DD58 pKa = 6.13DD59 pKa = 6.8DD60 pKa = 7.45DD61 pKa = 6.72DD62 pKa = 6.51DD63 pKa = 6.51GEE65 pKa = 4.41IVLPINSVRR74 pKa = 11.84LEE76 pKa = 4.11EE77 pKa = 4.11SSMWKK82 pKa = 10.27DD83 pKa = 3.07KK84 pKa = 11.14YY85 pKa = 11.05SPKK88 pKa = 10.03FDD90 pKa = 3.23GKK92 pKa = 10.18VNRR95 pKa = 11.84VDD97 pKa = 4.37RR98 pKa = 11.84KK99 pKa = 9.6NEE101 pKa = 3.8LYY103 pKa = 10.6ASRR106 pKa = 11.84VHH108 pKa = 5.38QNYY111 pKa = 9.0YY112 pKa = 10.79FMAPVFKK119 pKa = 10.81LIGLSCYY126 pKa = 9.74SNHH129 pKa = 5.34MHH131 pKa = 7.59GDD133 pKa = 3.22GDD135 pKa = 4.93DD136 pKa = 3.57IYY138 pKa = 11.54GPALNGTAMTTTFPTMTTLQAPAACTEE165 pKa = 4.16WEE167 pKa = 4.05GDD169 pKa = 4.15DD170 pKa = 3.9YY171 pKa = 12.09DD172 pKa = 4.13EE173 pKa = 4.59STTILTMILHH183 pKa = 5.29QQHH186 pKa = 7.26DD187 pKa = 4.27LMLIIVVHH195 pKa = 6.08GCC197 pKa = 3.36

Molecular weight: 21.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E4EKL8|A0A5E4EKL8_PRUDU Protein kinase domain-containing protein OS=Prunus dulcis OX=3755 GN=ALMOND_2B006539 PE=4 SV=1
MM1 pKa = 7.54SPPPKK6 pKa = 9.88HH7 pKa = 6.6KK8 pKa = 10.29PPPPQKK14 pKa = 10.22HH15 pKa = 5.77KK16 pKa = 10.48PPPPQKK22 pKa = 10.08HH23 pKa = 5.81KK24 pKa = 10.27PPPPPTPKK32 pKa = 10.31LNPPPPPNLSPPPPPPNLRR51 pKa = 11.84PPPPNLNPPPPPKK64 pKa = 10.41SNPRR68 pKa = 11.84PPPKK72 pKa = 9.97NRR74 pKa = 11.84PPPPPQNLNPPPPPQNLNPPPPPPQKK100 pKa = 9.46RR101 pKa = 11.84TPPPPPQRR109 pKa = 11.84IPPPNLIPPPPPPQNLNPPPQKK131 pKa = 9.48RR132 pKa = 11.84TPPPPPPRR140 pKa = 11.84RR141 pKa = 11.84PPPNLIPPPPPPQNLNPPPPPPKK164 pKa = 10.16KK165 pKa = 9.5LTPPPPQPRR174 pKa = 11.84RR175 pKa = 11.84PPPNLIPPPPPRR187 pKa = 11.84RR188 pKa = 11.84PPPNLIPPPPPPQNLNPPPPPPRR211 pKa = 11.84KK212 pKa = 8.75RR213 pKa = 11.84TPPPPPARR221 pKa = 11.84RR222 pKa = 11.84PPPNLIPPPPPAPKK236 pKa = 9.93QSPGTTATTPKK247 pKa = 9.72HH248 pKa = 5.72NPPVTPHH255 pKa = 6.47LMKK258 pKa = 10.87LHH260 pKa = 6.3

Molecular weight: 28.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27410

4524

31934

13217611

42

5365

413.9

46.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.686 ± 0.011

1.871 ± 0.007

5.295 ± 0.01

6.478 ± 0.016

4.184 ± 0.009

6.575 ± 0.013

2.456 ± 0.006

5.176 ± 0.01

6.15 ± 0.012

9.819 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.005

4.463 ± 0.007

4.959 ± 0.013

3.795 ± 0.01

5.257 ± 0.012

8.941 ± 0.015

4.865 ± 0.008

6.554 ± 0.009

1.303 ± 0.005

2.773 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski