Clostridium sp. CAG:524

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6C344|R6C344_9CLOT HolB OS=Clostridium sp. CAG:524 OX=1262817 GN=BN694_00238 PE=4 SV=1
MM1 pKa = 7.67NINGKK6 pKa = 9.78DD7 pKa = 3.34INIDD11 pKa = 3.5DD12 pKa = 5.45LFDD15 pKa = 3.26QKK17 pKa = 11.71YY18 pKa = 6.02MHH20 pKa = 7.0KK21 pKa = 9.63EE22 pKa = 3.3IKK24 pKa = 10.21KK25 pKa = 10.7GIFLSQYY32 pKa = 8.22QCEE35 pKa = 4.25VLQRR39 pKa = 11.84YY40 pKa = 8.25NINPYY45 pKa = 8.88EE46 pKa = 4.23CSSINEE52 pKa = 4.7LIFMIDD58 pKa = 3.16EE59 pKa = 4.22VLEE62 pKa = 4.66DD63 pKa = 4.43EE64 pKa = 5.59SDD66 pKa = 3.81ADD68 pKa = 4.12DD69 pKa = 4.83LDD71 pKa = 5.09GISRR75 pKa = 11.84EE76 pKa = 3.75ISEE79 pKa = 4.3FNYY82 pKa = 10.36YY83 pKa = 11.29ANTNKK88 pKa = 10.44

Molecular weight: 10.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6BGH7|R6BGH7_9CLOT Methyltransferase small domain protein OS=Clostridium sp. CAG:524 OX=1262817 GN=BN694_00240 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.45KK12 pKa = 10.75SKK14 pKa = 9.74TSGFLARR21 pKa = 11.84RR22 pKa = 11.84GTTVLKK28 pKa = 10.59ARR30 pKa = 11.84RR31 pKa = 11.84AKK33 pKa = 10.02GRR35 pKa = 11.84KK36 pKa = 8.37EE37 pKa = 3.5LAKK40 pKa = 10.74

Molecular weight: 4.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1265

0

1265

381930

34

2442

301.9

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.927 ± 0.06

1.142 ± 0.034

6.219 ± 0.058

7.105 ± 0.08

4.31 ± 0.045

5.206 ± 0.069

1.263 ± 0.027

10.279 ± 0.105

10.137 ± 0.077

8.802 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.032

7.851 ± 0.084

2.326 ± 0.037

1.645 ± 0.031

3.06 ± 0.05

6.662 ± 0.074

5.683 ± 0.079

5.961 ± 0.057

0.5 ± 0.02

5.49 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski