Clostridium phage phiCTC2A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7RUK5|A0A0A7RUK5_9CAUD Uncharacterized protein OS=Clostridium phage phiCTC2A OX=1567015 GN=phiCTC2A_18 PE=4 SV=1
MM1 pKa = 7.26NNIPEE6 pKa = 4.22CMYY9 pKa = 10.34DD10 pKa = 3.34YY11 pKa = 10.52RR12 pKa = 11.84YY13 pKa = 10.25EE14 pKa = 4.03FEE16 pKa = 4.52KK17 pKa = 10.36MQIIDD22 pKa = 3.56NCCNCDD28 pKa = 3.61CNICEE33 pKa = 4.19GEE35 pKa = 4.2EE36 pKa = 4.42YY37 pKa = 11.12YY38 pKa = 10.96DD39 pKa = 3.33IDD41 pKa = 3.76GTILCEE47 pKa = 3.83EE48 pKa = 5.17CIRR51 pKa = 11.84DD52 pKa = 3.83YY53 pKa = 11.64KK54 pKa = 9.91HH55 pKa = 5.51TAEE58 pKa = 4.36LL59 pKa = 3.79

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7RWT0|A0A0A7RWT0_9CAUD Terminase large subunit OS=Clostridium phage phiCTC2A OX=1567015 GN=phiCTC2A_31 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 9.93IEE4 pKa = 4.97KK5 pKa = 9.52IIKK8 pKa = 8.78AQQPDD13 pKa = 2.52IHH15 pKa = 7.01KK16 pKa = 10.39RR17 pKa = 11.84LKK19 pKa = 8.56QQNRR23 pKa = 11.84KK24 pKa = 9.2KK25 pKa = 9.82KK26 pKa = 8.65SRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.95GEE32 pKa = 4.37HH33 pKa = 6.75ISFSDD38 pKa = 3.19VMEE41 pKa = 4.24LMKK44 pKa = 10.41HH45 pKa = 6.35DD46 pKa = 3.34SYY48 pKa = 11.42KK49 pKa = 9.75RR50 pKa = 11.84HH51 pKa = 6.44RR52 pKa = 11.84GAIRR56 pKa = 11.84QRR58 pKa = 3.29

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13902

32

681

207.5

24.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.424 ± 0.403

1.108 ± 0.123

6.323 ± 0.259

8.517 ± 0.372

3.791 ± 0.155

5.424 ± 0.393

1.331 ± 0.118

9.071 ± 0.318

11.675 ± 0.405

8.394 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.148

7.323 ± 0.269

2.41 ± 0.161

3.007 ± 0.163

3.51 ± 0.234

5.193 ± 0.285

4.963 ± 0.238

4.999 ± 0.285

1.237 ± 0.105

4.719 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski