Candidatus Phytoplasma phoenicium
Average proteome isoelectric point is 8.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 327 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L0MJC7|A0A0L0MJC7_9MOLU Signal recognition particle receptor protein FtsY OS=Candidatus Phytoplasma phoenicium OX=198422 GN=ftsY PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.32 KK3 pKa = 9.56 MYY5 pKa = 9.83 IHH7 pKa = 6.65 RR8 pKa = 11.84 TTYY11 pKa = 10.76 YY12 pKa = 10.37 EE13 pKa = 3.99 NQNKK17 pKa = 7.46 ITYY20 pKa = 10.07 DD21 pKa = 3.69 NEE23 pKa = 4.02 FNDD26 pKa = 3.11 QGIIIKK32 pKa = 8.67 QIWYY36 pKa = 9.35 LPNSQDD42 pKa = 2.22 IDD44 pKa = 4.33 FIVEE48 pKa = 3.84 YY49 pKa = 10.82 DD50 pKa = 3.63 PLTGEE55 pKa = 4.48 EE56 pKa = 4.01 IDD58 pKa = 3.73 MEE60 pKa = 4.45 KK61 pKa = 10.77 LKK63 pKa = 9.58 EE64 pKa = 4.29 TYY66 pKa = 10.33 LVTVTNIDD74 pKa = 3.43 MGPSHH79 pKa = 6.88 EE80 pKa = 4.21 NN81 pKa = 2.98
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.289
IPC2_protein 4.469
IPC_protein 4.342
Toseland 4.19
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.19
Rodwell 4.19
Grimsley 4.101
Solomon 4.291
Lehninger 4.253
Nozaki 4.418
DTASelect 4.571
Thurlkill 4.202
EMBOSS 4.202
Sillero 4.457
Patrickios 3.032
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.395
Protein with the highest isoelectric point:
>tr|A0A0L0MK62|A0A0L0MK62_9MOLU Uncharacterized protein (Fragment) OS=Candidatus Phytoplasma phoenicium OX=198422 GN=AlmWB_01650 PE=4 SV=1
MM1 pKa = 7.3 ARR3 pKa = 11.84 IAGVDD8 pKa = 2.99 IPGNKK13 pKa = 8.43 RR14 pKa = 11.84 VVISLTYY21 pKa = 9.98 IYY23 pKa = 10.64 GIGLKK28 pKa = 8.47 TSRR31 pKa = 11.84 KK32 pKa = 9.17 LLCDD36 pKa = 3.17 LHH38 pKa = 8.15 IDD40 pKa = 3.54 EE41 pKa = 5.63 NIRR44 pKa = 11.84 VNKK47 pKa = 10.23 LSEE50 pKa = 4.17 EE51 pKa = 4.19 QLSHH55 pKa = 6.98 LRR57 pKa = 11.84 NEE59 pKa = 4.32 CTKK62 pKa = 10.91 LSVEE66 pKa = 3.91 GSLRR70 pKa = 11.84 RR71 pKa = 11.84 NITLNIKK78 pKa = 9.9 RR79 pKa = 11.84 LMEE82 pKa = 4.44 IGCDD86 pKa = 2.97 RR87 pKa = 11.84 GIHH90 pKa = 5.63 HH91 pKa = 7.25 RR92 pKa = 11.84 RR93 pKa = 11.84 GLPVRR98 pKa = 11.84 GQKK101 pKa = 8.12 TRR103 pKa = 11.84 NNAKK107 pKa = 7.94 TAKK110 pKa = 9.63 KK111 pKa = 9.72 RR112 pKa = 11.84 RR113 pKa = 11.84 KK114 pKa = 9.26 KK115 pKa = 10.61
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.765
IPC_protein 10.555
Toseland 11.052
ProMoST 10.833
Dawson 11.096
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.345
Grimsley 11.111
Solomon 11.286
Lehninger 11.242
Nozaki 11.023
DTASelect 10.804
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.038
Patrickios 11.082
IPC_peptide 11.286
IPC2_peptide 9.882
IPC2.peptide.svr19 8.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
327
0
327
94800
34
1364
289.9
33.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.121 ± 0.094
0.976 ± 0.041
4.261 ± 0.078
5.961 ± 0.133
5.896 ± 0.142
4.458 ± 0.121
2.223 ± 0.054
10.628 ± 0.132
10.481 ± 0.139
10.852 ± 0.124
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.999 ± 0.044
6.862 ± 0.11
2.991 ± 0.063
5.386 ± 0.133
2.917 ± 0.074
5.557 ± 0.095
5.052 ± 0.078
4.822 ± 0.086
0.617 ± 0.036
3.943 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here