Amino acid dipepetide frequency for Hymenobacter sp. PAMC 26554

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.691AlaAla: 16.691 ± 0.186
0.826AlaCys: 0.826 ± 0.028
6.291AlaAsp: 6.291 ± 0.076
6.12AlaGlu: 6.12 ± 0.087
3.711AlaPhe: 3.711 ± 0.055
10.067AlaGly: 10.067 ± 0.093
2.442AlaHis: 2.442 ± 0.048
4.114AlaIle: 4.114 ± 0.059
3.496AlaLys: 3.496 ± 0.06
12.793AlaLeu: 12.793 ± 0.124
1.795AlaMet: 1.795 ± 0.043
3.481AlaAsn: 3.481 ± 0.063
5.994AlaPro: 5.994 ± 0.078
5.634AlaGln: 5.634 ± 0.071
6.854AlaArg: 6.854 ± 0.091
5.658AlaSer: 5.658 ± 0.072
7.3AlaThr: 7.3 ± 0.1
7.593AlaVal: 7.593 ± 0.08
1.43AlaTrp: 1.43 ± 0.036
3.428AlaTyr: 3.428 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.667CysAla: 0.667 ± 0.026
0.1CysCys: 0.1 ± 0.01
0.347CysAsp: 0.347 ± 0.015
0.299CysGlu: 0.299 ± 0.015
0.306CysPhe: 0.306 ± 0.014
0.632CysGly: 0.632 ± 0.023
0.205CysHis: 0.205 ± 0.013
0.284CysIle: 0.284 ± 0.015
0.176CysLys: 0.176 ± 0.012
0.806CysLeu: 0.806 ± 0.026
0.095CysMet: 0.095 ± 0.007
0.231CysAsn: 0.231 ± 0.013
0.431CysPro: 0.431 ± 0.02
0.355CysGln: 0.355 ± 0.016
0.447CysArg: 0.447 ± 0.017
0.358CysSer: 0.358 ± 0.016
0.369CysThr: 0.369 ± 0.017
0.442CysVal: 0.442 ± 0.022
0.1CysTrp: 0.1 ± 0.01
0.272CysTyr: 0.272 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
5.078AspAla: 5.078 ± 0.059
0.32AspCys: 0.32 ± 0.017
2.228AspAsp: 2.228 ± 0.046
2.897AspGlu: 2.897 ± 0.051
2.545AspPhe: 2.545 ± 0.04
3.603AspGly: 3.603 ± 0.056
0.959AspHis: 0.959 ± 0.027
2.263AspIle: 2.263 ± 0.051
2.19AspLys: 2.19 ± 0.045
5.182AspLeu: 5.182 ± 0.069
0.881AspMet: 0.881 ± 0.028
1.802AspAsn: 1.802 ± 0.042
2.57AspPro: 2.57 ± 0.046
2.071AspGln: 2.071 ± 0.045
2.356AspArg: 2.356 ± 0.046
2.469AspSer: 2.469 ± 0.048
2.49AspThr: 2.49 ± 0.045
3.478AspVal: 3.478 ± 0.054
0.748AspTrp: 0.748 ± 0.025
2.24AspTyr: 2.24 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
5.883GluAla: 5.883 ± 0.09
0.26GluCys: 0.26 ± 0.014
1.857GluAsp: 1.857 ± 0.043
2.683GluGlu: 2.683 ± 0.055
2.009GluPhe: 2.009 ± 0.041
3.074GluGly: 3.074 ± 0.049
1.083GluHis: 1.083 ± 0.029
2.412GluIle: 2.412 ± 0.05
2.353GluLys: 2.353 ± 0.051
6.258GluLeu: 6.258 ± 0.087
1.147GluMet: 1.147 ± 0.031
1.772GluAsn: 1.772 ± 0.035
2.17GluPro: 2.17 ± 0.042
2.525GluGln: 2.525 ± 0.051
3.075GluArg: 3.075 ± 0.055
1.958GluSer: 1.958 ± 0.036
2.597GluThr: 2.597 ± 0.049
3.648GluVal: 3.648 ± 0.05
0.618GluTrp: 0.618 ± 0.023
1.474GluTyr: 1.474 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.933PheAla: 3.933 ± 0.06
0.336PheCys: 0.336 ± 0.015
2.414PheAsp: 2.414 ± 0.046
2.003PheGlu: 2.003 ± 0.038
1.862PhePhe: 1.862 ± 0.046
3.458PheGly: 3.458 ± 0.058
0.731PheHis: 0.731 ± 0.022
1.77PheIle: 1.77 ± 0.039
1.335PheLys: 1.335 ± 0.032
3.755PheLeu: 3.755 ± 0.057
0.758PheMet: 0.758 ± 0.019
1.611PheAsn: 1.611 ± 0.04
1.682PhePro: 1.682 ± 0.034
1.477PheGln: 1.477 ± 0.037
2.379PheArg: 2.379 ± 0.042
2.638PheSer: 2.638 ± 0.044
2.69PheThr: 2.69 ± 0.05
2.78PheVal: 2.78 ± 0.05
0.552PheTrp: 0.552 ± 0.02
1.518PheTyr: 1.518 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
7.109GlyAla: 7.109 ± 0.081
0.655GlyCys: 0.655 ± 0.022
3.19GlyAsp: 3.19 ± 0.055
3.5GlyGlu: 3.5 ± 0.052
3.177GlyPhe: 3.177 ± 0.053
6.574GlyGly: 6.574 ± 0.093
1.873GlyHis: 1.873 ± 0.036
3.668GlyIle: 3.668 ± 0.066
3.22GlyLys: 3.22 ± 0.054
8.945GlyLeu: 8.945 ± 0.099
1.563GlyMet: 1.563 ± 0.032
2.564GlyAsn: 2.564 ± 0.053
3.494GlyPro: 3.494 ± 0.058
4.37GlyGln: 4.37 ± 0.06
5.136GlyArg: 5.136 ± 0.065
4.553GlySer: 4.553 ± 0.076
5.185GlyThr: 5.185 ± 0.101
5.408GlyVal: 5.408 ± 0.07
1.173GlyTrp: 1.173 ± 0.033
3.059GlyTyr: 3.059 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.04HisAla: 2.04 ± 0.041
0.207HisCys: 0.207 ± 0.011
1.152HisAsp: 1.152 ± 0.033
1.142HisGlu: 1.142 ± 0.028
1.117HisPhe: 1.117 ± 0.028
1.649HisGly: 1.649 ± 0.035
0.645HisHis: 0.645 ± 0.025
0.856HisIle: 0.856 ± 0.026
0.646HisLys: 0.646 ± 0.028
2.677HisLeu: 2.677 ± 0.051
0.315HisMet: 0.315 ± 0.015
0.687HisAsn: 0.687 ± 0.021
1.352HisPro: 1.352 ± 0.035
1.044HisGln: 1.044 ± 0.026
1.298HisArg: 1.298 ± 0.035
1.054HisSer: 1.054 ± 0.027
1.125HisThr: 1.125 ± 0.029
1.233HisVal: 1.233 ± 0.03
0.356HisTrp: 0.356 ± 0.017
0.987HisTyr: 0.987 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
4.119IleAla: 4.119 ± 0.059
0.376IleCys: 0.376 ± 0.019
2.335IleAsp: 2.335 ± 0.044
2.303IleGlu: 2.303 ± 0.044
1.697IlePhe: 1.697 ± 0.039
3.5IleGly: 3.5 ± 0.059
0.757IleHis: 0.757 ± 0.021
2.208IleIle: 2.208 ± 0.051
1.795IleLys: 1.795 ± 0.038
3.711IleLeu: 3.711 ± 0.055
0.755IleMet: 0.755 ± 0.022
1.697IleAsn: 1.697 ± 0.041
1.961IlePro: 1.961 ± 0.043
1.573IleGln: 1.573 ± 0.036
2.379IleArg: 2.379 ± 0.045
2.644IleSer: 2.644 ± 0.051
2.85IleThr: 2.85 ± 0.049
2.765IleVal: 2.765 ± 0.053
0.452IleTrp: 0.452 ± 0.02
1.372IleTyr: 1.372 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.712LysAla: 3.712 ± 0.056
0.17LysCys: 0.17 ± 0.011
1.785LysAsp: 1.785 ± 0.046
1.895LysGlu: 1.895 ± 0.043
1.348LysPhe: 1.348 ± 0.031
2.576LysGly: 2.576 ± 0.051
0.687LysHis: 0.687 ± 0.023
1.851LysIle: 1.851 ± 0.043
1.985LysLys: 1.985 ± 0.051
3.949LysLeu: 3.949 ± 0.055
1.04LysMet: 1.04 ± 0.035
1.522LysAsn: 1.522 ± 0.037
1.973LysPro: 1.973 ± 0.044
1.645LysGln: 1.645 ± 0.038
1.86LysArg: 1.86 ± 0.043
1.874LysSer: 1.874 ± 0.036
2.283LysThr: 2.283 ± 0.048
2.46LysVal: 2.46 ± 0.05
0.374LysTrp: 0.374 ± 0.017
1.305LysTyr: 1.305 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
15.002LeuAla: 15.002 ± 0.149
0.737LeuCys: 0.737 ± 0.026
5.571LeuAsp: 5.571 ± 0.061
4.729LeuGlu: 4.729 ± 0.08
3.999LeuPhe: 3.999 ± 0.055
8.694LeuGly: 8.694 ± 0.099
2.618LeuHis: 2.618 ± 0.045
3.977LeuIle: 3.977 ± 0.06
3.854LeuLys: 3.854 ± 0.063
14.636LeuLeu: 14.636 ± 0.184
1.768LeuMet: 1.768 ± 0.036
4.011LeuAsn: 4.011 ± 0.062
7.414LeuPro: 7.414 ± 0.083
3.856LeuGln: 3.856 ± 0.059
8.314LeuArg: 8.314 ± 0.092
6.296LeuSer: 6.296 ± 0.07
7.516LeuThr: 7.516 ± 0.081
7.92LeuVal: 7.92 ± 0.093
1.09LeuTrp: 1.09 ± 0.036
3.118LeuTyr: 3.118 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
1.864MetAla: 1.864 ± 0.039
0.095MetCys: 0.095 ± 0.009
0.722MetAsp: 0.722 ± 0.024
0.783MetGlu: 0.783 ± 0.025
0.509MetPhe: 0.509 ± 0.018
1.282MetGly: 1.282 ± 0.035
0.392MetHis: 0.392 ± 0.015
0.571MetIle: 0.571 ± 0.019
0.989MetLys: 0.989 ± 0.031
2.109MetLeu: 2.109 ± 0.035
0.399MetMet: 0.399 ± 0.022
0.712MetAsn: 0.712 ± 0.021
1.188MetPro: 1.188 ± 0.031
0.901MetGln: 0.901 ± 0.028
1.21MetArg: 1.21 ± 0.027
1.105MetSer: 1.105 ± 0.027
1.065MetThr: 1.065 ± 0.026
1.082MetVal: 1.082 ± 0.029
0.148MetTrp: 0.148 ± 0.011
0.405MetTyr: 0.405 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.347AsnAla: 3.347 ± 0.058
0.257AsnCys: 0.257 ± 0.014
1.629AsnAsp: 1.629 ± 0.037
1.59AsnGlu: 1.59 ± 0.036
1.632AsnPhe: 1.632 ± 0.034
2.787AsnGly: 2.787 ± 0.058
0.707AsnHis: 0.707 ± 0.023
1.726AsnIle: 1.726 ± 0.04
1.296AsnLys: 1.296 ± 0.029
3.701AsnLeu: 3.701 ± 0.06
0.659AsnMet: 0.659 ± 0.02
1.444AsnAsn: 1.444 ± 0.048
2.36AsnPro: 2.36 ± 0.045
1.626AsnGln: 1.626 ± 0.037
1.895AsnArg: 1.895 ± 0.041
1.996AsnSer: 1.996 ± 0.05
2.074AsnThr: 2.074 ± 0.052
2.339AsnVal: 2.339 ± 0.044
0.474AsnTrp: 0.474 ± 0.019
1.472AsnTyr: 1.472 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
8.819ProAla: 8.819 ± 0.106
0.26ProCys: 0.26 ± 0.015
3.406ProAsp: 3.406 ± 0.051
3.044ProGlu: 3.044 ± 0.052
1.855ProPhe: 1.855 ± 0.037
4.787ProGly: 4.787 ± 0.054
1.136ProHis: 1.136 ± 0.03
1.92ProIle: 1.92 ± 0.038
1.625ProLys: 1.625 ± 0.034
5.854ProLeu: 5.854 ± 0.066
0.763ProMet: 0.763 ± 0.022
1.917ProAsn: 1.917 ± 0.041
3.221ProPro: 3.221 ± 0.062
1.954ProGln: 1.954 ± 0.04
2.737ProArg: 2.737 ± 0.048
2.426ProSer: 2.426 ± 0.044
4.256ProThr: 4.256 ± 0.062
4.2ProVal: 4.2 ± 0.06
0.623ProTrp: 0.623 ± 0.024
1.678ProTyr: 1.678 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
5.627GlnAla: 5.627 ± 0.073
0.22GlnCys: 0.22 ± 0.012
1.829GlnAsp: 1.829 ± 0.041
2.085GlnGlu: 2.085 ± 0.04
1.645GlnPhe: 1.645 ± 0.032
3.03GlnGly: 3.03 ± 0.049
1.221GlnHis: 1.221 ± 0.03
1.559GlnIle: 1.559 ± 0.034
1.57GlnLys: 1.57 ± 0.04
5.924GlnLeu: 5.924 ± 0.069
0.809GlnMet: 0.809 ± 0.023
1.513GlnAsn: 1.513 ± 0.037
3.071GlnPro: 3.071 ± 0.056
3.005GlnGln: 3.005 ± 0.053
3.229GlnArg: 3.229 ± 0.051
1.913GlnSer: 1.913 ± 0.036
2.513GlnThr: 2.513 ± 0.042
3.549GlnVal: 3.549 ± 0.056
0.548GlnTrp: 0.548 ± 0.023
1.417GlnTyr: 1.417 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
6.416ArgAla: 6.416 ± 0.075
0.385ArgCys: 0.385 ± 0.016
2.782ArgAsp: 2.782 ± 0.051
3.206ArgGlu: 3.206 ± 0.053
2.53ArgPhe: 2.53 ± 0.039
3.859ArgGly: 3.859 ± 0.057
1.579ArgHis: 1.579 ± 0.036
2.592ArgIle: 2.592 ± 0.046
1.938ArgLys: 1.938 ± 0.04
7.873ArgLeu: 7.873 ± 0.086
1.082ArgMet: 1.082 ± 0.028
1.91ArgAsn: 1.91 ± 0.036
3.766ArgPro: 3.766 ± 0.054
3.779ArgGln: 3.779 ± 0.066
4.722ArgArg: 4.722 ± 0.071
2.46ArgSer: 2.46 ± 0.038
3.341ArgThr: 3.341 ± 0.046
4.291ArgVal: 4.291 ± 0.048
0.902ArgTrp: 0.902 ± 0.026
2.423ArgTyr: 2.423 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
5.376SerAla: 5.376 ± 0.066
0.427SerCys: 0.427 ± 0.018
2.272SerAsp: 2.272 ± 0.043
2.25SerGlu: 2.25 ± 0.039
2.499SerPhe: 2.499 ± 0.051
4.485SerGly: 4.485 ± 0.075
1.035SerHis: 1.035 ± 0.028
2.311SerIle: 2.311 ± 0.046
1.767SerLys: 1.767 ± 0.038
6.026SerLeu: 6.026 ± 0.071
0.94SerMet: 0.94 ± 0.026
1.786SerAsn: 1.786 ± 0.044
3.05SerPro: 3.05 ± 0.049
2.269SerGln: 2.269 ± 0.041
2.937SerArg: 2.937 ± 0.05
3.069SerSer: 3.069 ± 0.054
3.166SerThr: 3.166 ± 0.062
3.574SerVal: 3.574 ± 0.046
0.755SerTrp: 0.755 ± 0.022
2.068SerTyr: 2.068 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
6.815ThrAla: 6.815 ± 0.078
0.369ThrCys: 0.369 ± 0.016
3.159ThrAsp: 3.159 ± 0.048
2.769ThrGlu: 2.769 ± 0.048
2.393ThrPhe: 2.393 ± 0.047
5.261ThrGly: 5.261 ± 0.081
1.198ThrHis: 1.198 ± 0.03
2.76ThrIle: 2.76 ± 0.057
2.087ThrLys: 2.087 ± 0.04
6.885ThrLeu: 6.885 ± 0.087
0.882ThrMet: 0.882 ± 0.027
2.185ThrAsn: 2.185 ± 0.055
4.347ThrPro: 4.347 ± 0.072
2.395ThrGln: 2.395 ± 0.046
3.006ThrArg: 3.006 ± 0.053
3.309ThrSer: 3.309 ± 0.067
4.355ThrThr: 4.355 ± 0.097
4.5ThrVal: 4.5 ± 0.073
0.745ThrTrp: 0.745 ± 0.021
2.228ThrTyr: 2.228 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
8.594ValAla: 8.594 ± 0.085
0.512ValCys: 0.512 ± 0.018
2.973ValAsp: 2.973 ± 0.045
3.35ValGlu: 3.35 ± 0.063
2.729ValPhe: 2.729 ± 0.046
5.564ValGly: 5.564 ± 0.066
1.144ValHis: 1.144 ± 0.029
2.896ValIle: 2.896 ± 0.047
2.438ValLys: 2.438 ± 0.048
8.211ValLeu: 8.211 ± 0.089
1.204ValMet: 1.204 ± 0.03
2.412ValAsn: 2.412 ± 0.049
4.005ValPro: 4.005 ± 0.052
2.921ValGln: 2.921 ± 0.049
4.88ValArg: 4.88 ± 0.067
3.854ValSer: 3.854 ± 0.057
3.904ValThr: 3.904 ± 0.065
5.741ValVal: 5.741 ± 0.071
0.76ValTrp: 0.76 ± 0.024
2.0ValTyr: 2.0 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.209TrpAla: 1.209 ± 0.036
0.095TrpCys: 0.095 ± 0.008
0.507TrpAsp: 0.507 ± 0.017
0.56TrpGlu: 0.56 ± 0.019
0.499TrpPhe: 0.499 ± 0.018
0.851TrpGly: 0.851 ± 0.029
0.369TrpHis: 0.369 ± 0.017
0.369TrpIle: 0.369 ± 0.016
0.389TrpLys: 0.389 ± 0.017
1.87TrpLeu: 1.87 ± 0.048
0.237TrpMet: 0.237 ± 0.015
0.43TrpAsn: 0.43 ± 0.019
0.626TrpPro: 0.626 ± 0.022
0.956TrpGln: 0.956 ± 0.027
0.911TrpArg: 0.911 ± 0.03
0.571TrpSer: 0.571 ± 0.019
0.655TrpThr: 0.655 ± 0.026
0.84TrpVal: 0.84 ± 0.028
0.216TrpTrp: 0.216 ± 0.014
0.367TrpTyr: 0.367 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.417TyrAla: 3.417 ± 0.045
0.312TyrCys: 0.312 ± 0.014
1.923TyrAsp: 1.923 ± 0.036
1.649TyrGlu: 1.649 ± 0.037
1.623TyrPhe: 1.623 ± 0.038
2.684TyrGly: 2.684 ± 0.046
0.802TyrHis: 0.802 ± 0.025
1.144TyrIle: 1.144 ± 0.029
1.143TyrLys: 1.143 ± 0.029
3.955TyrLeu: 3.955 ± 0.056
0.44TyrMet: 0.44 ± 0.017
1.325TyrAsn: 1.325 ± 0.036
1.806TyrPro: 1.806 ± 0.038
1.919TyrGln: 1.919 ± 0.037
2.212TyrArg: 2.212 ± 0.041
1.855TyrSer: 1.855 ± 0.043
1.91TyrThr: 1.91 ± 0.044
2.251TyrVal: 2.251 ± 0.042
0.459TyrTrp: 0.459 ± 0.018
1.492TyrTyr: 1.492 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4436 proteins (1456297 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski